[Tn5-mutagenesis of the styrene-degrading strain Pseudomonas sp. Y2. Analysis of transformation products and DNA-scopy of the mutants obtained]. / Tn5-mutagenez stiroldegradiruiushchego shtamma Pseudomonas sp. Y2. Analiz produktov transformatsii i DNK-skopiia poluchennykh mutantov.

The bacterium Pseudomonas sp. Y2 using styrene as a sole source of carbon and energy was subjected to transposon Tn5(Kmr) mutagenesis. The mutants were divided into three classes by the ability to grow on styrene, 2-phenylethanol, and phenylacetate. It is shown that 2-phenylethanol is not an obligate metabolite of styrene transformation. This allows the conclusion that in Pseudomonas sp. Y2 styrene can be degraded via consistent oxidation of the side chain by two pathways. For the comparison of strains/mutants and determination of genetic markers, DNA-fingerprinting of total DNA was carried out using electrophoresis of restriction fragments and blot-hybridization with different 32P-DNA-probes.

[Degradation of polycyclic aromatic hydrocarbons by a strain of Pseudomonas fluorescens 16N2]. / Degradatsiia politsiklicheskikh aromaticheskikh uglevodorodov shtammom Pseudomonas fluorescens 16N2.

The growth of Pseudomonas fluorescens 16N2 on naphthalene was accompanied with accumulation of salicylate in the culture medium and induction of gentisate 1,2-dioxygenase and catechol 1,2-dioxygenase. The transformation of anthracene by the cells growing on hexadecane led to the formation of 3-hydroxy-2-naphthoate and salicylate. Pathways for naphthalene and anthracene degradation are proposed.

[Biological methods of air purification (review of the literature)]. / Biologicheskie metody ochistki vozdukha (obzor).

Biological methods of air purification are reviewed, which can be applied to deep air purification of complex multicomponent mixtures from harmful, toxic and odorous substances at room temperature and atmospheric pressure. Microbiological and technological aspects of the problem are discussed. Operation characteristics of biofilters, bioscrubbers and trickle bed bioreactors are compared. Prospects of air biopurification are considered.