[Biochemical markers of apoptosis in different brain regions after training].

Activity of caspase-3 and content of DNA fragments with sizes 0.2-0.6 kbp and more than 4.0 kbp in the worm of the cerebellum, hippocampus and prefrontal cortex of the adult rat brain were estimated in 4 and 24 hours after the procedure of acoustic startle habituation and fear conditioning. Heterochronic changes in apoptotic markers in the examined brain structures were observed after training. Caspase-3 activity was decreased in the worm of the cerebellum and hippocampus, and DNA fragmentation was suppressed in the hippocampus and brain cortex. At the same time, both caspase activity and DNA fragmentation were increased in the hypothalamus. These results provide evidence for the involvement of apoptosis in the mechanisms of learning and memory in adult brain.

[Protein HLDF and antibodies to it as molecular pathogenetic factors and new markers of acute cerebral blood circulation disturbances]. / Belok HLDF i antitela k nemu kak molekuliarnye patogeneticheskie faktory i novye markery ostrykh narushenii mozgovogo krovoobrashcheniia.

Clinic-experimental complex investigation and ELISA determination of protein HLDF as also primary antibodies (Abs) to HLDF values in blood and cerebro-spinal fluid (CSF) of patients with hypertensive crises and acute aterotrombotic ischemic stroke were performed. Statistically improved difference in serum HLDF and Abs content in examined patients in comparison with age-matched controls as also significant intergroup's differences in protein and Abs dynamic content were determined. Correlation between content and dynamics of investigated factors in CSF and blood serum with determined clinical and instrumental parameters was revealed. Pathogenetic and prognostic significance of revealed changes in molecular factors was viewed.

[Effect of laser exposure on hemostatic parameters in the preagonal and post-resuscitation period]. / Deistvie lazernogo izlucheniia na parmetry gemostaza v preagonal'nom i postreanimatsionnom periode.

Effects of intravascular low-intensive laser exposure of the blood on the hemostasis during acute blood loss and the early postresuscitation period after 4-min clinical death were studied on narcotized dogs (8-17 kg) of both sexes with different initial levels of heparin. During the preagonal period laser exposure caused hypercoagulation in animals with initial heparin content below 60 micrograms/ml. This acceleration of blood clotting prevented a drop in the activity of antithrombin III and hypercoagulation by the third hour of postresuscitation period. In animals with initial heparin content more than 60 micrograms/ml laser exposure caused hypocoagulation in the presence of reduced activity of antithrombin III by the third hour of the resuscitation period.

[Hemocoagulation while dying of blood loss and restoration to life after clinical death]. / Gemokoaguliatsionnye parametry pri umiranii ot korovopoteri i ozhivlenii posle klinicheskio smerti.

Hemostatic system function was studied in dogs dying of acute blood loss and restoring to life after 4-min clinical death. Phasic changes in hemostatic system of two and three types occurred in the blood loss and reanimation, respectively. Dogs with favorable postresuscitation period exhibited hypercoagulation when dying, hypocoagulation 1 hour after reanimation and normal coagulation 3-6 hours after clinical death.

[The effect of low-intensity laser radiation on the parameters of the blood anticoagulation system in the early postresuscitation period]. / Vliianie nizkointensivnogo lazernogo izlucheniia na parametry protivosvertyvaiushchei sistemy krovi v rannem postreanimatsionnom periode.

The effect of low-intensive laser exposure at a wavelength of 634 nm and power 1 mWt at the tip of the light-guide on the blood anticoagulative system was studied in mongrel narcotized dogs of both sexes weighing 8 to 16 kg during the postresuscitation period after 4-min clinical death from massive blood loss. The anticoagulative system in the blood plasma was depleted during the reanimation period (the activities of plasmin and fibrinogen-heparin complexes and the level of heparin dropped). Intravascular laser exposure of the blood (for 30 min during blood loss after drop of the mean arterial pressure to 40 mm Hg and at the beginning of the second hour of the postreanimation period) boosted the activities of plasmin and fibrinogen-heparin complexes but failed to increase the level of heparin in the postreanimation period.

[The effect of thyroliberin on the structural characteristics of rat erythrocytes]. / Vliianie tiroliberina na strukturnye osobennosti éritrotsitov krys.

The effect of regulatory peptide thyrotropin-releasing hormone (TRH) on the structure of the plasma membrane and morphology of rat erythrocytes was studied using a spin probe and scanning electron microscopy. EPR spectra of the spin probe introduced in the intramembrane space demonstrate that TRH at 10(-7) and 10(-3) M induces structural changes in the erythrocyte membrane. Scanning electron microscopy showed that introduction of TRH at 10(-11), 10(-7), and 10(-3) M in the erythrocyte suspension increased the proportion of discocytes as compared to the control. This effect is due to TRH-induced cell transformation in discocytes. The obtained data suggest that TRH affects the membrane structure and the morphology of erythrocytes, changes the functional activity of these cells and, thus, indirectly influences the rate of the oxygen supply to tissue.

[Changes in the oxygen balance of the body in the acute period of hemorrhagic shock and after resuscitation under the effect of intravascular helium-neon laser irradiation of the blood]. / Izmeneniia kislorodnogo balansa organizma v ostrom periode gemorragicheskogo shoka i posle reanimatsii pod vliianiem vnutrisosudistogo gelii-neonovogo lazernogo oblucheniia krovi.

The effect of intravascular laser irradiation of the blood (ILIB) on the oxygen budget of the body and blood acid-base balance in the acute period of hemorrhagic shock and after resuscitation was studied in experiments on dogs. Duration of hypotension was 2 h. Laser therapy was started from the 5th-8th min of hypotension and lasted for 45 min; it was performed with a He-Ne laser (ALOK-1, 1 mWt power at the tip of the light guide, wavelength 633 nm) introduced in the right jugular vein. Exposure to ILIB in the acute period of hemorrhagic shock was conducive to creation of favorable conditions for realization of the compensatory mechanisms aimed at improvement of circulation and gas exchange in the lungs, at provision of delivery of oxygen to tissues and its utilization, and, hence, promoted the survival of animals by triggering the regulatory mechanisms of redistribution of the regional bloodflow, improvement of tissue perfusion, and possibly, of oxyhemoglobin dissociation processes in red cells.

[Experience with using anisotropic gradient filtration of tomographic images in the roentgenological diagnosis of diseases of the paranasal sinuses]. / Opyt ispol'zovaniia anizotropnoi gradientnoi fil'tratsii tomogramm v rentgenodiagnostike zabolevanii okolonosovykh pauz.

Radio- and tomogrammes of 335 patients with inflammatory and tumoral pathology of accessory nasal sinus were investigated. Anisotropic gradient filtration of the image, carried out with "Magiskan-2" apparatus was used. The procedure permitted to distinguish small unobstrusive changes in sinal bony tissues, routinely unnoticeable, and characteristic features of shadow structure, facilitating a differential diagnosis of accessory nasal sinus diseases.

[Characteristics of erythrocyte diagnostic agents studied using optical methods]. / Kharakteristika éritrotsitarnykh diagnostikumov opticheskimi metodami.

The quantitative characterization of erythrocyte diagnosticums (ED) has been made by optical methods (light microscopy with the use of an image analyzer, model Magiscan 2, and the opacity spectrum technique). The following parameters of ED have been determined: the average of the major axis (5.25 +/- 0.57 micron for ED from Shigella sonnei and 5.53 +/- 0.50 micron for ED from Shigella flexneri), the ratio of semiaxes (p approximately equal to 3), the major axis length distribution, the refractive index (1.076 +/- 0.002). For controlling the concentration of ED the use of the opacity spectrum technique is recommended.

[Study of the statistical characteristics of latex suspensions using the Magiscan-2 image analyser]. / Issledovanie statisticheskikh kharakteristik suspenzii lateksov s pomoshch'iu analizatora izobrazheniia "Magiskan-2".

Characteristics of latex suspensions are studied in order to estimate their potential metrologic application. The study is carried out on the set "Magiscan 2" with the standard programs. Histograms for distribution of perimeters and particle diameters are determined, and the correlation between them is specified. Also presented are the histograms for distributing optical particle density. The correlation between optical parameters and the geometry of particles is found as well as the orientation and correlation between the optical density of particles and their location on the microscopic stage.