[Directed biosynthesis of prostaglandin F2 alpha multiple labeled with tritium]. / Napravlennyi biosintez kratnomechennogo tritiem prostaglandina F2 alpha.

By selecting specific activators of PGF reductase and determining their optimal ratio, we have performed the directed biosynthesis of PGF2 alpha with a 45-50% yield. With the use of [5,6,8,9,11,12,14,15-3H8]arachidonic acid, multiple-labelled PGF2 alpha with molar radioactivity 6.1 TBe/mmol has been synthesised.

[Isolation and characteristics of immobilized preparations of prostaglandin synthetase]. / Poluchenie i kharakteristika immobilizovannykh preparatov prostaglandinsintetazy.

Enzymatic preparations of prostaglandin synthetase were immobilized by various methods, i.e., covalent binding to the carrier, incorporation into the matrix of polyacrylamide gel and adsorption on ion-exchangers. The most representative results were obtained with the use of the absorption technique. In this case, the enzyme activity either decreased very insignificantly or remained unaffected. The stability of immobilized enzyme preparations was studied. Some kinetic characteristics of immobilized prostaglandin synthetase were obtained.