Assuntos
Intoxicação por Arsênico , Bactérias/efeitos dos fármacos , Resistência a Múltiplos Medicamentos/genética , Bactérias/genética , Bactérias/isolamento & purificação , Resistência Microbiana a Medicamentos/genética , Enterobacter/efeitos dos fármacos , Klebsiella pneumoniae/efeitos dos fármacos , Peso Molecular , PlasmídeosRESUMO
The recombinant plasmid pKC47M has been constructed on the base of a mini-Mu-phage and the replicon of plasmid pBR328. The plasmid was constructed for in vivo insertional mutagenesis in bacteria. Use of the plasmid in vivo alleviates the possibility of secondary transposition of the inserted sequences and enrichment of multiple-site mutations. Efficient mutagenesis caused by plasmid pKC47M was tested by induction auxotrophic mutations in Escherichia coli, Yersinia enterocolitica, Yersinia pseudotuberculosis.
Assuntos
DNA Recombinante/genética , Mutagênese Insercional , Bacteriófago mu/genética , Escherichia coli/genética , Plasmídeos , Transformação Genética , Yersinia enterocolitica/genética , Yersinia pseudotuberculosis/genéticaRESUMO
A set of isogenic derivatives of Yersinia pestis EV strain was obtained including the variants harbouring the different compositions of Yersinia own plasmids. The protein profiles of outer membranes of the set of strains were defined. The polyacrylamide gel electrophoresis has shown the small 6.1 Md plasmid to code an outer membrane protein with mol mass 29 kDa, different from pesticin I, while the heavy 60.0 Md plasmid encodes the 15-16 kDa polypeptide different from monomers of F1 and T-antigens of plague microbe.
