[Periodic changes in the morphophysiological properties of chemostat culture of Candida utilis yeasts under alternating temperatures]. / Periodicheskie izmeneniia morfofiziologicheskikh svoistv khemostatnoi kul'tury drozhzhei Candida utilis v usloviiakh peremennykh temperatur.

The object of this work was to find out whether it was possible to cultivate yeasts in chemostat in varying regime: at multiple changes to temperature from the optimal one (31 degrees C) to the supraoptimal one (37 degrees C) and back with a frequency comparable to the generation time; this regime was alternated during 32 generations. The cultures were compared with chemostat cultures grown in steady-state regimes at 31 or 37 degrees C. The value of Y, the composition of cells and morphometric characteristics were determined by the optical-structural computer analysis. The size and shape of cells and the optical properties of the protoplasm were found to be in the oscillatory regime correlating with changes in the growth temperature and periodically tending to the normal characteristics. The value of Y, the content of RNA and protein in the biomass gradually stabilized. A possibility, in principal, to continuously cultivate yeasts is discussed when the conditions of the environment are rhythmically changed and the process is controlled in the morpho-physiological characteristics of the cells.

[Study of lymphoid tissue cells by the method of optico-structural machine analysis]. / Izuchenie kletok limfoidnoi tkani metodom optiko-strukturnogo mashinnogo analiza.

The possibility to use the method of optically-structural machine analysis for automatical classification of lymphoid cells of tumour-bearing rats and lymphoid cells of malignant tumour was studied. The preparations were fixed by methanol, stained by azureosin and scanned on the automatical microscope-analysor "Protva-3". The isolated cells of 6 different groups were scanned. Histogramms of optical density distribution amplitudes were received for every cell and the phase analysis was carried out. The statistical characterization for every cell was based on the results of this analysis. The average values were calculated for 1) the area of the nucleus and cytoplasm: 2) the average density of nucleus and cytoplasm; 3) dispersion of densities of nucleus and cytoplasm; 4) nucleus-cytoplasm relation for every six groups. It was shown that every group of cells may be separated from each other, at least, on the basis of one of the measured parameters. The results are consistent with the morphological data.