[The effect of desferal on the cellular proliferative activity of the mouse spleen, thymus and bone marrow]. / Vliianie desferala na proliferativnuiu aktivnost' kletok selezenki, timusa i kostnogo mozga myshei.

CBA mice were given a single Desferal dose (50 mg/kg). The mice received 3H-Thymidine 1 hour before they were killed. They were killed 1, 2, 6, or 9 hours after Desferal. Radiometry, and autoradiography were used to determine the level of DNA synthesis. Desferal was found to stimulate splenic 3H-incorporation 3 hours after drug injection and to increase the DNA-synthesizing cell fraction. In contrast, the agent inhibited 3H-incorporation in the bone marrow and thymus 9 hours after injection. This suppressive affect was accompanied by a decrease in the DNA-synthesizing cell fraction in these organs. In the thymus, there was a significant increase in the DNA-synthesizing cell fraction among lymphoblasts 1 and 3 hours following the administration of Desferal.

[Effects of high and low molecular soluble factors of the bone marrow on antibody formation and pain sensitivity in animals]. / Vliianie vysoko- i nizkomolekuliarnykh rastvorimykh faktorov kostnogo mozga na antiteloobrazovanie i bolevuiu chuvstvitel'nost' u zhivotnykh.

In addition to the immunostimulating activity, bone marrow mediators, myelopeptides (MP) show the dose-dependent effect on the development of pain sensitivity in mice. When injected in nanogram amounts, MP induce hyperalgesia and 3-9 fold higher production of antibodies against SREC. When injected in milligram amounts, they exhibit hypoalgesic effect and no influence on antibody production. Immunostimulating effect in MP (mol, mass less than 1 KD, fraction 3) is accompanied with hypoalgesia. Bone marrow factors of mol. masses 40-150 KD (fraction 1) eluted at Sephadex G-25 gel-filtration before MP enhance the pain sensitivity tHreshold and show a potent immunodepressive effect. Thus the bone marrow factors are capable of exhibiting the opposite effects on the immune system in the pain control system that evidences the tight interrelation between these systems.

[Effect of vinblastine on the proliferative and immune responses of the lymphoid cells of the draining lymph node and spleen in mice undergoing regional immunization with ram erythrocytes]. / Vliianie vinblastina na proliferativnyi i immunnyi otvet limfoidnykh kletok dreniruiushchego limfaticheskogo uzla i selezenki myshei pri regionarnoi immunizatsii éritrotsitami barana.

It was found that vinblastine administered 48 hours after immunization of mice by sheep red cells produced a long-term (for up to 3 days) suppression of 3H-thymidine inclusion into lymphoid cells following a 8-12-hour latent period. This was accompanied with a complete elimination of humoral response of the draining lymph node and a five-fold decrease of the number of antibody-forming cells in the spleen.