[HPLC separation and characterization of tick-borne encephalitis and equine Venezuela encephalomyelitis viral proteins]. / Razdelenie s pomoshch'iu VEZhKh belkov virusov kleshchevogo éntsefalita i venesuél'skogo éntsefalomielita loshadeí i ikh stroenie.

Homogeneous (according to PAGE) capsid and surface viral proteins were isolated from concentrated purified suspensions of tick-borne encephalitis and Venezuelan equine encephalomyelitis viruses by one-stage reversed-phase HPLC. The amino acid composition and the sequences of their N-terminal parts were determined.

[High performance liquid chromatography and characterization of structural proteins of the influenza virus]. / Vysokoéffektivnaia zhidkostnaia khromatografiia i kharakterizatsiia strukturnykh belkov virusa grippa.

Constituent proteins of human influenza virus A have been separated by reverse-phase HPLC on Polysil ODS-500. Their homogeneity is confirmed by the data of amino acid composition, Edman analysis and gel electrophoresis.

[Rapid automated synthesis on paper disks of oligodeoxyribonucleotides constituting the promoter fragment of the vaccinia virus genome]. / Bystryi avtomatizirovannyi sintez na bumazhnykh diskakh oligodeoksiribonukleotidov, sostavliaiushchikh promotornyi fragment genoma virusa ospovaktsiny.

For automation of segmental solid-phase synthesis a simple approach leading to the optimal scheme of synthesis of a large numbers of oligonucleotides in one reaction vessel has been proposed. An advantage of the scheme as compared with synthesis in four reaction vessels is a lower number of condensation steps and increased economy of the process. Sixteen oligodeoxyribonucleotides constituting promoter fragment of the viral genome have been synthesised by the modified segmental method on "Victoriya-2" synthesizer according to the optimal scheme.

[A simplified variant of the Maxam-Gilbert method for determining the primary structure of oligonucleotides and DNA fragments]. / Uproshchennyi variant metoda Maksama-Gilberta dlia opredeleniia pervichnoi struktury oligonukleotidov i fragmentov DNK.

A modification to the Maxam-Gilbert method is proposed that involves precipitation of the nucleotide material with the acetone solution of lithium perchlorate after the completion of chemical reactions to remove the reagents. Modification of cytosine residues is carried out in the presence of lithium chloride. The new mode of precipitation simplifies and speeds up the analysis of oligonucleotides and DNA fragments.