Assuntos
Linfoma de Células T/diagnóstico , Idoso , Edema/etiologia , Feminino , Humanos , Invasividade Neoplásica , NarizRESUMO
No disponible
Assuntos
Humanos , Feminino , Idoso , Linfoma Cutâneo de Células T/patologia , Neoplasias Cutâneas/patologia , Granuloma Letal da Linha Média/patologia , Linfócitos do Interstício Tumoral/patologia , Músculo Esquelético/patologiaRESUMO
Detection of genetic variability in Cercospora kikuchii isolates from a single soybean field. Current knowledge about epidemiology and population structure of Cercospora kikuchii is little developed and no studies regarding this subject have been reported in Argentina. The aim of this work was to select primers to study genetic variability in C. kikuchii isolated from the same soybean field using RAPD (Random Amplified Polymorphism DNA). RAPD was applied to the DNA of 5 C. kikuchii, isolated from diseased tissue of the soybean in the same field, another isolate, from a strain collection. Out of seven primers, five of them proved to be useful to study the population of C. kikuchii isolates.
Assuntos
Ascomicetos/genética , Variação Genética , Glycine max/microbiologia , Ascomicetos/isolamento & purificaçãoRESUMO
El conocimiento de la epidemiología y la estructura poblacional de Cercospora kikuchii está poco desarrollado y no se han comunicado estudios al respecto en la Argentina. El objetivo de este trabajo fue seleccionar oligonucleótidos que permitan detectar variabilidad genética en aislamientos de C. kikuchii obtenidos a partir de soja proveniente de un mismo sembradío, mediante la aplicación de RAPD. Se trabajó con 6 aislamientos de C. kikuchii, 5 de ellos se obtuvieron a partir de trozos de tejido enfermo y el restante provenía de una colección de cultivos. De los 7 oligonucleótidos empleados, 5 resultaron útiles para el estudio poblacional de los aislamientos de C. kikuchii.
Current knowledge about epidemiology and population structure of Cercospora kikuchii is little developed and no studies regarding this subject have been reported in Argentina. The aim of this work was to select primers to study genetic variability in C. kikuchii isolated from the same soybean field using RAPD (Random Amplified Polymorphism DNA). RAPD was applied to the DNA of 5 C. kikuchii, isolated from diseased tissue of the soybean in the same field, another isolate, from a strain collection. Out of seven primers, five of them proved to be useful to study the population of C. kikuchii isolates.
Assuntos
Ascomicetos/genética , Variação Genética , Glycine max/microbiologia , Ascomicetos/isolamento & purificaçãoRESUMO
Strictosidine beta-D-glucosidase (SGD) is an enzyme involved in the biosynthesis of terpenoid indole alkaloids (TIAs) by converting strictosidine to cathenamine. The biosynthetic pathway toward strictosidine is thought to be similar in all TIA-producing plants. Somewhere downstream of strictosidine formation, however, the biosynthesis diverges to give rise to the different TIAs found. SGD may play a role in creating this biosynthetic diversity. We have studied SGD at both the molecular and enzymatic levels. Based on the homology between different plant beta-glucosidases, degenerate polymerase chain reaction primers were designed and used to isolate a cDNA clone from a Catharanthus roseus cDNA library. A full-length clone gave rise to SGD activity when expressed in Saccharomyces cerevisiae. SGD shows approximately 60% homology at the amino acid level to other beta-glucosidases from plants and is encoded by a single-copy gene. Sgd expression is induced by methyl jasmonate with kinetics similar to those of two other genes acting prior to Sgd in TIA biosynthesis. These results show that coordinate induction of the biosynthetic genes forms at least part of the mechanism for the methyl jasmonate-induced increase in TIA production. Using a novel in vivo staining method, subcellular localization studies of SGD were performed. This showed that SGD is most likely associated with the endoplasmic reticulum, which is in accordance with the presence of a putative signal sequence, but in contrast to previous localization studies. This new insight in SGD localization has significant implications for our understanding of the complex intracellular trafficking of metabolic intermediates during TIA biosynthesis.
