Pattern and value of Sperm DNA Fragmentation Index and its correlation with spermiogram in infertile South West Nigerian Men.

Semen analysis has been used for a long time to assess male fertility due to its limitations sperm DNA fragmentation index (DFI), which describes the sperm DNA's condition, is an appropriate criterion for assessing male fertility. This study evaluated the pattern and value of DFI of infertile men in the South West of Nigeria. This is a cross-sectional and descriptive study that recruited two hundred and eighty-seven (287) patients from two fertility centers in Lagos, Nigeria. The Sperm DFI was determined using the Sperm chromatin structure assay (SCSA) test. The descriptive and inferential statistics of the study were carried out using R packages (R version 4.2.0) with the help of R functions using compiled code. The result showed that the mean age sperm concentration, total motility morphology, and DFI were as follows 42.96±7.09years, 40.18±4.19×106 per ml, 49%±19%, 56±17%, and 15.78±8.52 respectively. There is a significant negative correlation between sperm concentration and DFI at a P-value of 0.0018 with a regression model of Coefficient of determination is 0.305. The DFI value of infertile men negatively correlates with sperm concentration, thus increase sperm production may improve sperm quality.

The spermiogram and correlation of seminal fluid parameters in patient attending fertility centre in Lagos, South West Nigeria.

BACKGROUND: The determinant of the spermiogram of semen varies in different populations based on several factors ranging, from age to the pathological state of an individual to environmental factors. The aim of the study is to determine the spermiogram of patients that attend fertility clinics in southwest Nigeria and the relationship between the parameters. METHODOLOGY: This is a cross-sectional study that recruited two hundred and ninety seven (297) patients from two fertility centers in Lagos, Nigeria for the period of January 2021 to November 2022. The sperm samples were collected following WHO standards. The spermiogram was analyzed using an automated sperm analyzer and the descriptive and inference statistics of the study were carried out using R packages (R version 4.2.0). RESULTS: The result showed the mean age of 43.12±6.95years with median age of 42years. The mean of sperm count and concentration were 114×106 sperm cells and 42×106 per mL with the mean volume of the semen produced by the patients was 2.69mL and average motility (progressive and non-progressive) of the sperm is 47%±19%, 42%±17% has normal morphology. The distributions of the observed variables (seminal fluid parameters) were different from normal distributions in the studied population, such that almost all of them are skewed to the right. The degree of relationship between the sperm parameter were very weak. Nevertheless, specifically, there is a negative correlation between age and sperm count, age and motility, age and volume, and a positive correlation between age and abnormal morphology. The results showed that sperm morphology has a significant effect on motility while sperm morphology significantly depend on sperm count. CONCLUSION: An increase in sperm volume and concentration improves the sperm morphology and boost the sperm motility, this may increasing the chance of fertility.

Relationship between Renal Doppler Indices and Biochemical indices of Renal Function in Type 2 Diabetes Mellitus.

OBJECTIVES: Diabetic nephropathy is a common complication of diabetes mellitus due to microangiopathy leading to end stage renal disease. This study determined the relationship between renal resistivity index and pulsatility index with biochemical indices of renal function in patients with type 2 diabetes mellitus methods: This study involved 80 adults with type 2 diabetes mellitus. Urinary albumin excretion rate (UAER) and serum creatinine levels were measured, and the estimated glomerular filtration rate (eGFR) was calculated. Right renal resistivity index (RI) and pulsatility index (PI) values were determined. RESULTS: Mean renal resistivity index was 0.72±0.06 while the pulsatility index was 1.36 ± 0.24. Resistivity index was positively correlated with albuminuria (r = 0.426; p <0.001) and serum creatinine (r = 0.458; p <0.001), but negatively correlated with eGFR (r = -0.399; p <0.001). There was positive correlation between pulsatility index and albuminuria (r = 0.341; p = 0.002), and serum creatinine (r = 0.478; p = <0.001); and negative correlation between PI and eGFR (r = - 0.359; p = 0.001). CONCLUSIONS: Renal resistivity index and pulsatility index may provide valuable non-invasive estimate of predicting the presence and severity of renal dysfunction in patients with type 2 diabetes.

Variations of renal vascular anatomy in a nigerian population: A computerized tomography studys.

BACKGROUND: A broad spectrum of renal vascular variations has been reported by anatomists and radiologists. The prevalence of these variations is extremely divergent in different populations. Therefore, radiologists and surgeons in different climes must be knowledgeable about the type and prevalence of the variants in their area of practice to avoid diagnostic pitfalls and for optimization of surgical techniques. OBJECTIVE: The objective of this study is to describe the types and prevalence of renal vascular variations among patients undergoing contrast-enhanced computerized tomography (CECT) of the abdomen in a Nigerian population, as well as provide a concise review of literature on the embryological basis and clinical significance of the identified variations. MATERIALS AND METHODS: This study was a retrospective review of 200 CECT of the abdomen to identify variations of arterial (accessory, early branching, and precaval) and venous (multiple, retroaortic, and circumaortic) anatomy of the kidneys. RESULTS: We studied 200 patients, 102 (51%) females and 98 (49%) males. Age range is 18-90 years (mean = 53.08 ± 17.01). Prevalence of any renal vascular variations was 50%, arterial variations were 37%, and venous variations were 13%. Variations were significantly more common in males, P = 0.000075. The most common arterial variant was the accessory renal artery (23%) seen in 10% (right) and 13.0% (left); early branching was seen in 4.0% (right) and 0.5% (left) as well as precaval right renal artery seen in 4.5%. Venous variants were late confluence 3.0% (right) and 2.5% (left); multiple veins was seen in 2.5% (right) and 2.5% (left) as well as retroaortic left renal vein seen in 2.0%. The inferior polar accessory artery was the most prevalent accessory artery. Early arterial bifurcation was significantly more common on the right (P = 0.016) while other vascular variants showed no statistically significant association with laterality. CONCLUSION: Variation of renal vascular anatomy is a frequent finding among Nigerians. Radiologists and surgeons must be aware of these variants for optimization of surgical techniques.

Radiation dose and radiation protection principle awareness: a survey among Nigerian paediatricians.

AIMS AND OBJECTIVES: This study is aimed at determining the knowledge of Paediatricians in Nigeria about the basic principle of radiation protection ALARA (As Low As Reasonably Achievable) and their knowledge of the radiation doses that children receive during some common radiological procedures. MATERIALS AND METHODS: Two hundred and fifty questionnaires were circulated among paediatricians at the 2012 annual Paediatricians' Association of Nigeria Conference. The questionnaires contain 10 questions designed to asses the pediatricians' general knowledge on : ionising radiation and the risks, doses children receive during some common radiological procedures and awareness of the radiation protection principle, ALARA ( As Low As Reasonably Achievable). RESULTS: Of the 162 Paediatricians that participated, 69% named at least one non medical source of ionising radiation, 54.9% would not recommend CXR to screen an apparently healthy child for tuberculosis and 87% believe that children are at greater risk of adverse effects of ionising radiation. For dose estimation, 51.9% and 51.2% of the paediatricians underestimated doses received during Cranial and abdominal computerised tomography respectively while 13.6% and 37% respectively erroneously believed that abdominal ultrasound and brain magnetic resonance imaging utilise ionising radiation. 13.6% gave the correct meaning of the Acronym ALARA. CONCLUSIONS: The Paediatricians' knowledge about the basic principle of radiation protection ALARA and the doses that children receive during some common radiological procedures is poor. There is need to ensure adequate training on radiation hazards and protection at all levels of medical education.

Neglected massive intracerebral abscess: an unusual cause of bilateral visual loss.

BACKGROUND: Bilateral visual loss following chronically raised intracranial pressure is not uncommon especially in developing countries. However, this calls for concern when the cause of the raised intracranial pressure is neglected pyogenic cerebral abscess. CASE SUMMARY: A sixteen year old male student presented to our unit with eight months history of recurrent headache associated with early morning vomiting. He developed bilateral visual loss a month prior to presentation. He had sought treatment at several herbal homes and orthodox hospitals before presentation. His cranial computed tomographic scan (CT) showed a massive peripheral contrast enhancing lesion in the frontal lobes with gross midline shift and other evidence of mass effect. He subsequently had craniotomy and excision of the mass. Intraoperative and laboratory findings confirmed the lesions to be abscess. He had ciprofloxacin and metronidazole for four weeks. He had uneventful postoperative period though his visual loss has persisted. He is being followed up in the clinic. CONCLUSION: This patient illustrates that untreated frontal lobe abscess could cause bilateral visual loss from chronically elevated intracranial pressure (ICP). Even though this should be rare in contemporary neurosurgical practice, ignorance and poverty which are rife in developing countries will be important contributory factors. Physicians working in developing countries should be aware of the symptomatology of cerebral abscess and raised ICP. They should encourage patients to do cranial CT when the features are suggestive of space occupying lesions.

The appearances of benign breast diseases on ultrasound.

BACKGROUND: Benign breast diseases are common and Ultrasound is a very useful tool in examining benign breast diseases especially in distinguishing solid from cystic masses. This study aims to determine the sonographic pattern of benign breast diseases in a Nigerian population and to compare this pattern with that which has been previously reported in other environments. METHODS: This a prospective study of Ninety-four patients with breast disease scanned between 1997 and 2001 on request from the breast clinic of the Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife. A total of forty-four patients with histologicaly confirmed benign breast diseases were selected for this study. Their ultrasonographic features were evaluated and compared with those previously described. RESULT: Fibroadenoma was the commonest disease, occurring in 62.2% of the patients, with a mean age of 29.1 years, an age higher than that previously reported. Other diseases which were expected to occur commonly (breast abscess, cyst) were rarely seen. CONCLUSION: While it was discovered that the sonographic pattern of most of the diseases in this study conformed to that already described in literature despite late presentation, only one sonographic appearance of galactocele, out of the three reported, was noted in this study. In addition, despite the nonspecificity of ultrasound appearances in fibrocystic disease, an attempt has been made to match these appearances with the different histological types already described in literature.