RESUMO
Some types of actinomycetes produce many different secondary metabolites of fatty acids, hydrocarbons, or other compounds. Many of these products play an important role in various medical fields. This study aims to extract natural compounds from actinomycetes after their isolation from the soil and their identification as antimicrobials. Soil samples were collected from different regions after being treated by known methods. Colonies that indicate actinomycetes were replanted and provided with suitable conditions for growth, and then tested against a number of pathogenic microbes. Isolate 3-D is more effective than others. D-3 was exposed to ultraviolet rays for greater production of antimicrobials. The compounds obtained from the isolates were extracted by the column chromatography technique. To identify the compounds resulting from the extract, the Gas Chromatography-Mass Spectrometry (GC-MS) technique was used. Ten compounds have been identified by GC-MS. Some of the compounds are of fatty acid nature, and some are hydrocarbons. These compound includes Hexadecane, 2,6,11,15- Tetramethyl - Octacosane - Dodecanoic Acid, 1,2,3- Propane-triyl ester - Hexatriacontane - Heptacosane - Eicosyl Acetate - Tritetracontane - Tetracosane, 2,6,10,15,19,23-Hexamethyl - Myristic Acid vinyl ester - Tetratetracontane. All of these extracts are of medical importance. Some of them are anti-bacterial, some are anti-allergic, anti-inflammatory, anti-cancer, anti-fungal, antioxidants, and some of them are essential ingredients in cosmetics. The current study showed that isolated D-3 actinomycetes from soil have the ability to produce antimicrobials against a variety of gram-negative and gram-positive bacteria, which are important in the medical field.
RESUMO
Actinomycetes have been identified as an origin of many secondary metabolites, antibiotics and active components that impact microbial growth. Mediated mutations using UV in practice for the breeding of organisms. The objective of this study is to analyses the impact of UV radiation on the (O-7) Actinomycete isolate. This was a prospective analytical study of a several of actinomycetes. The isolates were screened for antimicrobial efficacy against multiple Gram-positive, Gram-negative bacteria, yeast, and fungi. Various factors such as UV, temperature, pH, light, agitation, fermentation durations and aeration have also been boosted for optimal antimicrobial production. The isolate (O-7) Actinomycete has been recognized as a highly bioactive producing organism. The isolate was exposed to various wavelengths, times under numerous growth conditions. It was found that 4% concentration of glucose as a carbon source is significantly optimal for the production of antibiotic for (O-7) UV exposed strain, however, concentration of 1% of lactose is significantly optimal for the production of antibiotic for (O-7) UV exposed strain. Yeast extract at a concentration of 1% was found to be the best source of nitrogen for (O-7) UV exposed, while pH 7.0 was found to be the most suitable for the same isolate. From the temperature optimization study, it was observed that (O-7) exposed strain showed good growth and maximum antibiotic production at 28 °C. The soil-isolated biological compounds (O-7) were effective against certain types of bacteria and fungi, and the research also demonstrated that exposure to UV radiation enhanced the production of these compounds.
