RESUMO
Porphyromonas gingivalis gingipains is suspected to be one of the most important causative agents of periodontitis. We postulated that the inhibition of gingipains may reduce the pathogenic nature of P. gingivalis. Anti-P. gingivalis egg yolk antibody (IgY-GP) was isolated from the yolks of hens immunized with purified gingipains. We applied IgY-GP gel subgingivally in periodontitis patients who harbored P. gingivalis in their subgingival flora. Five pairs of contralateral anterior single-rooted teeth were selected. One tooth in each contralateral pair was randomly treated with IgY-GP and subgingival scaling and root planing, whereas the other tooth was treated with SRP alone. The number of P. gingivalis bacteria was assessed by real-time PCR. Bacterial levels were expressed as the percentage of total bacteria. The IgY-GP group had a significant reduction in probing depth. BOP significantly decreased in the IgY-GP group compared to the control group at week 4. The levels of P. gingivalis significantly increased in the control group at week 4, whereas the reduction in the levels of P. gingivalis was sustained in the IgY-GP group. Within the limitations of the present study, IgY-GP was shown to be an effective immunotherapeutic agent in the treatment of periodontitis.
Assuntos
Adesinas Bacterianas/efeitos dos fármacos , Cisteína Endopeptidases/efeitos dos fármacos , Gema de Ovo/imunologia , Imunoglobulinas/farmacologia , Periodontite/microbiologia , Periodontite/terapia , Porphyromonas gingivalis/enzimologia , Inibidores de Proteases/farmacologia , Idoso , DNA Bacteriano/análise , Raspagem Dentária , Cisteína Endopeptidases Gingipaínas , Humanos , Imunização Passiva , Imunoglobulinas/uso terapêutico , Pessoa de Meia-Idade , Inibidores de Proteases/uso terapêutico , Estatísticas não ParamétricasRESUMO
The pathogenicities of RacL11 and Kentucky D strains of equine herpesvirus 1 in the hamster infection model are different from those of Ab4p and the Japanese isolates. Virus genome restriction fragment length polymorphism analysis and sequence comparison of an intergenic region, glycoproteins and tegument genes showed higher conservation but with some strain-specific differences. These results indicate that point nucleotide differences in RacL11 and Kentucky D might be responsible for their pathogenicity in rodent models.
Assuntos
Genes Virais/genética , Variação Genética , Herpesvirus Equídeo 1/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Sequência Conservada/genética , Cricetinae , Herpesvirus Equídeo 1/patogenicidade , Dados de Sequência Molecular , Polimorfismo de Fragmento de Restrição , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
Interspecies trasmission of equine herpesvirus 1 (EHV-1) from horse to cattle was shown by Crandell et al. (1988). Specific mutations related to the transmission were studied by comparison of five EHV-1 isolates in cattle (BH1247, 3M20-3, G118, G1753, and 9BSV4) using polymerase chain reaction and restriction fragment length polymorphism analysis with added sequencing. G118 and 3M20-3 were the genome type EHV-1 P, while G1753 was the genome type EHV-1 B. BH1247 and 9BSV4 might be other genome types. We could not identify specific mutations related to the interspecies transmission.
Assuntos
Doenças dos Bovinos/virologia , Infecções por Herpesviridae/virologia , Herpesvirus Equídeo 1/genética , Mutação , Animais , Sequência de Bases , Bovinos , Doenças dos Bovinos/transmissão , Cricetinae , Impressões Digitais de DNA , Transferência Genética Horizontal , Infecções por Herpesviridae/transmissão , Herpesvirus Equídeo 1/isolamento & purificação , Herpesvirus Equídeo 1/patogenicidade , Cavalos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/métodos , Polimorfismo de Fragmento de Restrição , VirulênciaRESUMO
Herpesviral DNA was detected in 24/261 DNA samples that were extracted from whole blood of 13 wild animal species in South Africa. Herpesviral DNA was shown in 22 impalas (Aepyceros melampus) and 2 springboks (Antidorcas marsupialis). All of DNA sequences detected in impalas were identical, whereas two DNA sequences detected in springboks were different each other. These three sequences showed 44 to 72% homology to the corresponding gene of the Gammaherpesvirinae, indicating that these three viruses should be unrecognized novel gammaherpesviruses. The putative novel herpesviruses were tentatively designated as Aepyceros melampus (impala) herpesvirus 1 (ImHV-1), Antidorcas marsupialis (springbok) herpesviruses 1 (SpHV-1) and 2 (SpHV-2). ImHV-1 seems to be a relatively independent virus. SpHV-1 belongs to a group of ruminant lymphotropic herpesviruses and SpHV-2 is closer to a malignant catarrhal fever virus group. Potential threat of these herpesviruses to domestic animals should be considered.
