RESUMO
OBJECT: We previously reported that erythromycin (EM) affected the adhesion molecules that interact with neutrophils and inhibited neutrophil infiltration in a rat experimental otitis media model. PURPOSE: The purpose of the present study was to examine whether EM affects the adhesion of rat neutrophils to endothelial cells. METHODS: The adhesion of neutrophils to WK-5 endothelial cells was evaluated. One group of rats was given EM for 2 weeks, while the other group received no treatment. The adhesion of peripheral-blood neutrophils and neutrophils accumulated in the middle ear from these two groups were compared. RESULTS: Two-week EM pretreatment inhibited adhesion of neutrophils to WK-5 cells. The adhesion of neutrophils accumulated in the middle ear cavity to WK-5 cells was significantly higher than that of peripheral-blood neutrophils from control rats. CONCLUSION: The results suggest that EM affects the adhesion of neutrophils to endothelial cells.
Assuntos
Antibacterianos/farmacologia , Células Endoteliais/efeitos dos fármacos , Endotélio Vascular/efeitos dos fármacos , Eritromicina/farmacologia , Neutrófilos/efeitos dos fármacos , Otite Média/tratamento farmacológico , Animais , Antibacterianos/uso terapêutico , Adesão Celular/efeitos dos fármacos , Modelos Animais de Doenças , Endotélio Vascular/citologia , Eritromicina/uso terapêutico , Selectina L/análise , Selectina L/efeitos dos fármacos , Antígeno de Macrófago 1/análise , Antígeno de Macrófago 1/efeitos dos fármacos , Distribuição Aleatória , Ratos , Ratos Sprague-DawleyRESUMO
To elucidate the mechanisms involved in the action of new macrolides on chronic sinusitis, we examined the effects of clarithromycin (CAM) and roxiythromycin (RXM) on the expression of adhesion molecules (L-selectin and Mac-1) on peripheral blood neutrophils of individuals with chronic sinusitis. The administration of CAM and RXM slightly inhibited the down-regulation of L-selectin expression on neutrophils induced by interleukin (IL)-8 stimulation. Furthermore, the administration of CAM strongly inhibited the IL-8-induced up-regulation of Mac-1 expression on neutrophils. These observations suggest that the new macrolides such as CAM and RXM may affect the functions of neutrophils in chronic sinusitis by modulating the expression of L-selectin and Mac-1 molecules on neutrophils, thereby attenuating the adhesion of neutrophils.
Assuntos
Claritromicina/uso terapêutico , Selectina L/análise , Antígeno de Macrófago 1/análise , Neutrófilos/efeitos dos fármacos , Roxitromicina/uso terapêutico , Sinusite/tratamento farmacológico , Adolescente , Adulto , Idoso , Doença Crônica , Claritromicina/efeitos adversos , Regulação para Baixo/efeitos dos fármacos , Feminino , Humanos , Interleucina-8/farmacologia , Masculino , Pessoa de Meia-Idade , Neutrófilos/imunologia , Roxitromicina/efeitos adversos , Sinusite/imunologia , Regulação para Cima/efeitos dos fármacosRESUMO
Immunohistochemistry is useful to establish the pathological diagnosis of nasal glioma for which surgical excision is the unique treatment. Here we report a case of intranasal glioma found in a 5-month-old male patient. The tumor was successfully removed by endoscopic surgery. In the absence of a connection between the tumor and endocranial contents, we suggest to remove intranasal glioma by endoscopic surgery.
