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1.
Ageing Res Rev ; 86: 101880, 2023 04.
Artigo em Inglês | MEDLINE | ID: mdl-36773760

RESUMO

Numerous pesticides are inhibitors of the oxidative phosphorylation system. Oxidative phosphorylation dysfunction adversely affects neurogenesis and often accompanies Parkinson disease. Since brain development occurs mainly in the prenatal period, early exposure to pesticides could alter the development of the nervous system and increase the risk of Parkinson disease. Different rodent models have been used to confirm this hypothesis. However, more precise considerations of the selected strain, the xenobiotic, its mode of administration, and the timing of animal analysis, are necessary to resemble the model to the human clinical condition and obtain more reliable results.


Assuntos
Doença de Parkinson , Praguicidas , Animais , Gravidez , Feminino , Humanos , Doença de Parkinson/etiologia , Praguicidas/toxicidade , Neurogênese/fisiologia
2.
Redox Biol ; 41: 101871, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33540295

RESUMO

Down syndrome is the most common genomic disorder of intellectual disability and is caused by trisomy of chromosome 21. Several genes in this chromosome repress mitochondrial biogenesis. The goal of this study was to evaluate whether early overexpression of these genes may cause a prenatal impairment of oxidative phosphorylation negatively affecting neurogenesis. Reduction in the mitochondrial energy production and a lower mitochondrial function have been reported in diverse tissues or cell types, and also at any age, including early fetuses, suggesting that a defect in oxidative phosphorylation is an early and general event in Down syndrome individuals. Moreover, many of the medical conditions associated with Down syndrome are also frequently found in patients with oxidative phosphorylation disease. Several drugs that enhance mitochondrial biogenesis are nowadays available and some of them have been already tested in mouse models of Down syndrome restoring neurogenesis and cognitive defects. Because neurogenesis relies on a correct mitochondrial function and critical periods of brain development occur mainly in the prenatal and early neonatal stages, therapeutic approaches intended to improve oxidative phosphorylation should be provided in these periods.


Assuntos
Síndrome de Down , Animais , Modelos Animais de Doenças , Síndrome de Down/metabolismo , Humanos , Recém-Nascido , Camundongos , Mitocôndrias/metabolismo , Neurogênese , Fosforilação Oxidativa
3.
Mutat Res Rev Mutat Res ; 786: 108334, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33339579

RESUMO

Dilated cardiomyopathy is a frequent and extremely heterogeneous medical condition. Deficits in the oxidative phosphorylation system have been described in patients suffering from dilated cardiomyopathy. Hence, mutations in proteins related to this biochemical pathway could be etiological factors for some of these patients. Here, we review the clinical phenotypes of patients harboring pathological mutations in genes related to the oxidative phosphorylation system, either encoded in the mitochondrial or in the nuclear genome, presenting with dilated cardiomyopathy. In addition to the clinical heterogeneity of these patients, the large genetic heterogeneity has contributed to an improper allocation of pathogenicity for many candidate mutations. We suggest criteria to avoid incorrect assignment of pathogenicity to newly found mutations and discuss possible therapies targeting the oxidative phosphorylation function.


Assuntos
Cardiomiopatia Dilatada/genética , Cardiomiopatia Dilatada/patologia , DNA Mitocondrial/genética , Humanos , Mitocôndrias/genética , Mitocôndrias/patologia , Mutação , Fosforilação Oxidativa , Fenótipo
4.
Int J Mol Sci ; 21(9)2020 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-32397676

RESUMO

Mitochondrial oxidative phosphorylation disorders are extremely heterogeneous conditions. Their clinical and genetic variability makes the identification of reliable and specific biomarkers very challenging. Until now, only a few studies have focused on the effect of a defective oxidative phosphorylation functioning on the cell's secretome, although it could be a promising approach for the identification and pre-selection of potential circulating biomarkers for mitochondrial diseases. Here, we review the insights obtained from secretome studies with regard to oxidative phosphorylation dysfunction, and the biomarkers that appear, so far, to be promising to identify mitochondrial diseases. We propose two new biomarkers to be taken into account in future diagnostic trials.


Assuntos
DNA Mitocondrial/genética , Fatores de Crescimento de Fibroblastos/metabolismo , Fator 15 de Diferenciação de Crescimento/metabolismo , Interleucina-6/metabolismo , Doenças Mitocondriais/metabolismo , Fosforilação Oxidativa , Fator A de Crescimento do Endotélio Vascular/metabolismo , Biomarcadores/metabolismo , Fatores de Crescimento de Fibroblastos/genética , Fator 15 de Diferenciação de Crescimento/genética , Humanos , Doenças Mitocondriais/genética , Via Secretória/efeitos dos fármacos , Via Secretória/genética , Fator A de Crescimento do Endotélio Vascular/genética
5.
Cells ; 8(11)2019 11 08.
Artigo em Inglês | MEDLINE | ID: mdl-31717322

RESUMO

Neuronal differentiation appears to be dependent on oxidative phosphorylation capacity. Several drugs inhibit oxidative phosphorylation and might be detrimental for neuronal differentiation. Some pregnant women take these medications during their first weeks of gestation when fetal nervous system is being developed. These treatments might have later negative consequences on the offspring's health. To analyze a potential negative effect of three widely used medications, we studied in vitro dopaminergic neuronal differentiation of cells exposed to pharmacologic concentrations of azidothymidine for acquired immune deficiency syndrome; linezolid for multidrug-resistant tuberculosis; and atovaquone for malaria. We also analyzed the dopaminergic neuronal differentiation in brains of fetuses from pregnant mice exposed to linezolid. The drugs reduced the in vitro oxidative phosphorylation capacity and dopaminergic neuronal differentiation. This differentiation process does not appear to be affected in the prenatally exposed fetus brain. Nevertheless, the global DNA methylation in fetal brain was significantly altered, perhaps linking an early exposure to a negative effect in older life. Uridine was able to prevent the negative effects on in vitro dopaminergic neuronal differentiation and on in vivo global DNA methylation. Uridine could be used as a protective agent against oxidative phosphorylation-inhibiting pharmaceuticals provided during pregnancy when dopaminergic neuronal differentiation is taking place.


Assuntos
Neurônios Dopaminérgicos/citologia , Neurônios Dopaminérgicos/efeitos dos fármacos , Neurônios Dopaminérgicos/metabolismo , Fármacos Neuroprotetores/farmacologia , Fosforilação Oxidativa/efeitos dos fármacos , Uridina/farmacologia , Xenobióticos/farmacologia , Animais , Biomarcadores , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/genética , Linhagem Celular , Células Cultivadas , Metilação de DNA , Glucose/farmacologia , Humanos , Imuno-Histoquímica , Camundongos , Mitocôndrias/genética , Mitocôndrias/imunologia
6.
Aging (Albany NY) ; 11(19): 8433-8462, 2019 09 27.
Artigo em Inglês | MEDLINE | ID: mdl-31560653

RESUMO

Many patients suffering late-onset Alzheimer disease show a deficit in respiratory complex IV activity. The de novo pyrimidine biosynthesis pathway connects with the mitochondrial respiratory chain upstream from respiratory complex IV. We hypothesized that these patients would have decreased pyrimidine nucleotide levels. Then, different cell processes for which these compounds are essential, such as neuronal membrane generation and maintenance and synapses production, would be compromised. Using a cell model, we show that inhibiting oxidative phosphorylation function reduces neuronal differentiation. Linking these processes to pyrimidine nucleotides, uridine treatment recovers neuronal differentiation. To unmask the importance of these pathways in Alzheimer disease, we firstly confirm the existence of the de novo pyrimidine biosynthesis pathway in adult human brain. Then, we report altered mRNA levels for genes from both de novo pyrimidine biosynthesis and pyrimidine salvage pathways in brain from patients with Alzheimer disease. Thus, uridine supplementation might be used as a therapy for those Alzheimer disease patients with low respiratory complex IV activity.


Assuntos
Doença de Alzheimer , Complexo IV da Cadeia de Transporte de Elétrons/fisiologia , Neurônios/fisiologia , Fosforilação Oxidativa/efeitos dos fármacos , Pirimidinas/biossíntese , Uridina , Doença de Alzheimer/tratamento farmacológico , Doença de Alzheimer/metabolismo , Encéfalo/metabolismo , Diferenciação Celular/efeitos dos fármacos , Desenho de Fármacos , Humanos , Mitocôndrias/metabolismo , Fármacos Neuroprotetores/farmacologia , Transdução de Sinais/efeitos dos fármacos , Uridina/metabolismo , Uridina/farmacologia
7.
Ageing Res Rev ; 45: 24-32, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29689408

RESUMO

Late-onset Parkinson disease is a multifactorial and multietiological disorder, age being one of the factors implicated. Genetic and/or environmental factors, such as pesticides, can also be involved. Up to 80% of dopaminergic neurons of the substantia nigra are lost before motor features of the disorder begin to appear. In humans, these neurons are only formed a few weeks after fertilization. Therefore, prenatal exposure to pesticides or industrial chemicals during crucial steps of brain development might also alter their proliferation and differentiation. Oxidative phosphorylation is one of the metabolic pathways sensitive to environmental toxicants and it is crucial for neuronal differentiation. Many inhibitors of this biochemical pathway, frequently found as persistent organic pollutants, affect dopaminergic neurogenesis, promote the degeneration of these neurons and increase the risk of suffering late-onset Parkinson disease. Here, we discuss how an early, prenatal, exposure to these oxidative phosphorylation xenobiotics might trigger a late-onset, old age, Parkinson disease.


Assuntos
Fosforilação Oxidativa/efeitos dos fármacos , Doença de Parkinson Secundária/metabolismo , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente , Efeitos Tardios da Exposição Pré-Natal/metabolismo , Xenobióticos/efeitos adversos , Idade de Início , Dopamina/metabolismo , Neurônios Dopaminérgicos/efeitos dos fármacos , Neurônios Dopaminérgicos/metabolismo , Neurônios Dopaminérgicos/patologia , Feminino , Humanos , Neurogênese/efeitos dos fármacos , Neurogênese/fisiologia , Doença de Parkinson Secundária/epidemiologia , Gravidez , Efeitos Tardios da Exposição Pré-Natal/epidemiologia , Substância Negra/efeitos dos fármacos , Substância Negra/metabolismo , Substância Negra/patologia
8.
J Alzheimers Dis ; 42(1): 87-96, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25024340

RESUMO

We present a new hypothesis on the contribution of a dysfunction of the oxidative phosphorylation system, through a decrease in the de novo synthesis of pyrimidine nucleotides, to the pathogenesis of late onset Alzheimer's disease (AD). In the light of this proposition, different treatments for AD patients, such as enhancing the electron flow downstream the coenzyme Q10 of the mitochondrial respiratory chain or increasing mitochondrial biogenesis or directly providing pyrimidines, would be possible. AD is a multifactorial disorder and not all patients would benefit from these treatments. Those healthy individuals harboring mtDNA haplotypes related to a coupled OXPHOS function would probably be the better candidates for these preventive therapies.


Assuntos
Doença de Alzheimer/tratamento farmacológico , Doença de Alzheimer/genética , Fosforilação Oxidativa/efeitos dos fármacos , Nucleotídeos de Pirimidina , Doença de Alzheimer/metabolismo , Animais , DNA Mitocondrial/metabolismo , Humanos , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Nootrópicos/farmacologia , Nootrópicos/uso terapêutico , Farmacogenética/métodos , Nucleotídeos de Pirimidina/biossíntese
9.
Metab Brain Dis ; 28(4): 639-45, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23612782

RESUMO

Multiple sclerosis is likely caused by a complex interaction of multiple genes and environmental factors. The contribution of mitochondrial DNA genetic backgrounds has been frequently reported. To evaluate the effect of mitochondrial DNA haplogroups in the same genetic and environmental circumstances, we have built human transmitochondrial cell lines and simulated the effect of axon demyelination, one of the hallmarks of multiple sclerosis pathology, by altering the ionic gradients through the plasmalemma and increasing ATP consumption. In this model, mitochondrial biogenesis is observed. This process is larger in Uk cybrids, which mirrors their lower oxidative phosphorylation capacity in basal conditions. This model replicates a process occurring in both patients suffering from multiple sclerosis and several animal models of axon demyelination. Therefore, it can be used to analyze the contribution of various mitochondrial DNA genotypes to multiple sclerosis. In this sense, a longer or stronger energy stress, such as that associated with demyelinated axons in multiple sclerosis, could make Uk individuals more susceptible to this pathology. Finally, pharmacologic compounds targeted to mitochondrial biogenesis could be a potential therapy for multiple sclerosis.


Assuntos
Axônios/patologia , DNA Mitocondrial , Mitocôndrias/patologia , Esclerose Múltipla/patologia , Bainha de Mielina/patologia , Axônios/metabolismo , Linhagem Celular , Sobrevivência Celular/fisiologia , Haplótipos , Humanos , Mitocôndrias/genética , Mitocôndrias/metabolismo , Esclerose Múltipla/genética , Esclerose Múltipla/metabolismo , Bainha de Mielina/genética , Bainha de Mielina/metabolismo , Consumo de Oxigênio/fisiologia
10.
Hum Mol Genet ; 22(6): 1132-9, 2013 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-23223015

RESUMO

Some ribosomal antibiotics used in clinical practice to fight pathogenic bacteria can provoke serious adverse drug reactions in patients. Sensitivity to the antibiotics is a multifactorial trait but the genetic variation of sensitive individuals to off-target effects of the drugs might be one of the factors contributing to this condition. Thus, the protein synthesis apparatus of mitochondria is similar to that of bacteria because of its endosymbiotic origin and, therefore, mitochondrial ribosomes are frequently unintended off-targets of these antibiotics. Because of the limitations of epidemiologic studies of pharmacogenomics, we constructed 25 transmitochondrial cell lines using platelets from individuals belonging to high-frequency European mitochondrial DNA (mtDNA) haplogroups and grew them in the absence or presence of commonly used ribosomal antibiotics. Next, we analyzed the mitochondrial synthesis of proteins and the mitochondrial oxygen consumption to ascertain whether some side effects of ribosomal drugs are due to their interaction with particular mtDNA haplogroup-defining polymorphisms. The amount of mitochondrial translation products, the p.MT-CO1/succinate dehydrogenase subunit A ratio and the ratio of respiratory complex IV quantity to citrate synthase (CS)-specific activity were significantly lower, after the treatment with linezolid, in cybrids harboring the highly frequent m.3010A allele. These results suggest that mitochondrial antibiograms should be implemented for at least the most frequent mitochondrial ribosomal RNA (rRNA) polymorphisms and combinations of polymorphisms and the most frequently used ribosomal antibiotics. In this way, we would obtain individualized barcodes for antibiotic therapy, avoid the side effects of the antibiotics and enable appropriate personalized medicine.


Assuntos
Antibacterianos/uso terapêutico , Infecções Bacterianas/tratamento farmacológico , Testes de Sensibilidade Microbiana , Mitocôndrias/efeitos dos fármacos , Medicina de Precisão , Infecções Bacterianas/genética , Infecções Bacterianas/metabolismo , Linhagem Celular , Humanos , Mitocôndrias/genética , Mitocôndrias/metabolismo , Dados de Sequência Molecular , Biossíntese de Proteínas/efeitos dos fármacos
11.
Mutat Res ; 728(3): 98-106, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21763451

RESUMO

Activities and quantities of several oxidative phosphorylation (OXPHOS) system complexes are decreased in many Parkinson's disease (PD) patients. Mutations in PD-associated nuclear genes affect OXPHOS function. Moreover, the inactivation of other nuclear genes related to mitochondrial DNA (mtDNA) replication and expression also leads to Parkinsonism. MtDNA only encodes OXPHOS subunits and the RNAs required for their expression. Mutations in mtDNA genes have also been associated with PD. Furthermore, many xenobiotics that inhibit different OXPHOS complexes provoke Parkinsonism. The binding sites for these venoms are usually mtDNA-encoded subunits. However, and despite the existence of mutations or toxicants that can cause Parkinsonism, PD only rarely results from isolated genetic or environmental factors. Combinations of nuclear and mitochondrial genetic and environmental factors have additive effects and increase the risk of PD. It is also possible that population polymorphisms in mtDNA genes, affecting interactions with different xenobiotics, may behave as susceptibility factors for developing PD only in the presence of that particular xenobiotic. Therefore, a deeper analysis of the OXPHOS function in PD is required if we want to unravel the complexities of this disorder.


Assuntos
Interação Gene-Ambiente , Fosforilação Oxidativa , Doença de Parkinson/etiologia , DNA Mitocondrial/fisiologia , Humanos , Mutação , Doença de Parkinson/genética , Doença de Parkinson/metabolismo , Toxicogenética , Xenobióticos/efeitos adversos
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