Effects of ivermectin on development of Calliphora vicina, Robineau-Desvoidy 1830 (Diptera, Calliphoridae).

Ivermectin is one of the most widely used drugs for parasite control. Previous studies have shown a reduction in the abundance and diversity of "non-target" coprophilous organisms due to the presence of ivermectin (IVM) in bovine faecal matter (FM). Due to its breadth of behavioural habits, Calliphora vicina is a suitable dipteran species to evaluate the effects of IVM in FM. The aim of this work was to evaluate the effect of five concentrations of IVM in FM (3000, 300, 100, 30, and 3 ng/g) on the development of C. vicina. The following endpoints were evaluated: survival (between the first larval stage and emergence of new adults), larval development times to pupation and pupation times to adult, and adult emergence (% sex) and LC50. Sampling was performed from larval hatching at 60 and 120 min and at 3, 4, 5, and 12 h, and every 24 h specimens were weighed until pupae were observed. Data were analysed by ANOVA using a non-parametric Kruskal-Wallis test and as a function of elapsed development time and accumulated degree hours (ADH). Mortality at 3000 and 300 ng/g was 100% and 97%, respectively. There were statistically significant delays in adult emergence time (p = 0.0216) and in the ADH (p = 0.0431) between the control group (C) and 100 ng/g. The LC50 was determined at 5.6 ng/g. These results demonstrate the lethal and sub-lethal effects of IVM on C. vicina, while highlighting the usefulness of this species as a bioindicator for ecotoxicological studies.

Toward Reliable Synthesis of Superconducting Infinite Layer Nickelate Thin Films by Topochemical Reduction.

Infinite layer (IL) nickelates provide a new route beyond copper oxides to address outstanding questions in the field of unconventional superconductivity. However, their synthesis poses considerable challenges, largely hindering experimental research on this new class of oxide superconductors. That synthesis is achieved in a two-step process that yields the most thermodynamically stable perovskite phase first, then the IL phase by topotactic reduction, the quality of the starting phase playing a crucial role. Here, a reliable synthesis of superconducting IL  nickelate films is reported after successive topochemical reductions of a parent perovskite phase with nearly optimal stoichiometry. Careful analysis of the transport properties of the incompletely reduced films reveals an improvement in the strange metal behavior of their normal state resistivity over subsequent topochemical reductions, offering insight into the reduction process.

[Gastrocolic fistula as infrequent cause of chronic diarrhea in a patient with percutaneous radiological gastrostomy]. / Fístula gastrocólica como causa infrecuente de diarrea persistente en un paciente portador de gastrostomía radiológica percutánea.

Introduction: Introduction: gastrocolic fistula is an infrequent but severe complication of percutaneous gastrostomy. Clinical suspicion in the presence of chronic diarrhea of unknown etiology manifesting after percutaneous radiological gastrostomy (PRG) tube replacement is key to early detection and treatment. Case report: we report the case of a patient with PRG that began with chronic diarrhea after tube replacement and developed severe malnutrition. Initial treatment was not effective, studies were extended with the finding of this complication in a CT image. The use of this tube was discontinued with resolution of diarrhea and a favorable nutritional outcome. Discussion: this case report shows the importance of considering gastrocolic fistula in the differential diagnosis of persistent diarrhea in a patient with a gastrostomy tube.

Introducción: Introducción: la fístula gastrocólica supone una complicación infrecuente pero potencialmente grave de las sondas de gastrostomía. La sospecha clínica ante una diarrea de origen incierto que comienza tras el recambio de la sonda es clave para la detección y el tratamiento precoces. Caso clínico: se presenta el caso de un paciente portador de gastrostomía radiológica percutánea (PRG) que comienza con diarrea persistente tras el primer recambio de la sonda y desnutrición grave secundaria. Tras el fracaso de las medidas terapéuticas iniciales se amplían los estudios, con hallazgo de esta complicación en la imagen de TC. Se suspende el uso de esta sonda con resolución de la diarrea y evolución nutricional favorable. Discusión: este caso pone de manifiesto la importancia de incluir la fístula gastrocólica en el diagnóstico diferencial de la diarrea persistente en un paciente portador de sonda de gastrostomía.

Trapping Behaviour of Duddingtonia flagrans against Gastrointestinal Nematodes of Cattle under Year-Round Grazing Conditions.

The purpose of using nematophagous fungi as biological control agents of gastrointestinal nematodes of livestock is to reduce the build-up of infective larvae on pasture and thus avoid clinical and subclinical disease. As the interaction of fungus-larval stages takes place in the environment, it is crucial to know how useful the fungal agents are throughout the seasons in areas where livestock graze all year-round. This study was designed to determine the predatory ability of the nematophagous fungus Duddingtonia flagrans against gastrointestinal nematodes of cattle during four experiments set up in different seasons. In each experiment, faeces containing eggs of gastrointestinal nematodes were mixed with 11,000 chlamydospores/g and deposited on pasture plots. A comparison between fungal-added faeces and control faeces without fungus were made with regard to pasture infectivity, larval presence in faecal pats, faecal cultures, faecal pat weight, and temperature inside the faecal mass. In three of the four experiments, Duddingtonia flagrans significantly reduced the population of infective larvae in cultures (68 to 97%), on herbage (80 to 100%), and inside the faecal pats (70 to 95%). The study demonstrated the possibility of counting on a biological control tool throughout most of the year in cattle regions with extensive grazing seasons.

Breakfast Size and Prevalence of Metabolic Syndrome in the European Prospective Investigation into Cancer and Nutrition (EPIC) Spanish Cohort.

BACKGROUND: Recent evidence suggest that energy distribution during the daytimecould be a potential determinant for the development of metabolic syndrome (MetS). OBJECTIVE: To cross-sectionally assess the association between breakfast size and the prevalence of MetS in Spanish adults. METHODS: Our study included a subset of 3644 participants from the European Prospective Investigation into Cancer and Nutrition Spain study recontacted between 2017-2018. Information on diet, sociodemographic, lifestyle, sleep quality, and chronotype was collected using standardized questionnaires, while anthropometric and blood pressure data were measured in a face-to-face personal interview by a nurse. MetS was defined according to the Adult Treatment Panel III (ATPIII) definition by measuring serum levels of total cholesterol, tryglycerides and glucose. Breakfast size was calculated as: (energy from breakfast/total energy intake) * 2000 kcal. To evaluate the association between breakfast size and MetS prevalence, a multivariable logistic regression model adjusted by potential confounders was used to estimate OR and 95% CI. RESULTS: Prevalence of MetS in our study was 40.7%. The mean breakfast size was 306.6 * 2000 kcal (15% of the total daily energy intake), with 14 (0.4%) participants skipping breakfast. Participants in the highest quartile of breakfast size had a lower MetS prevalence compared to participants in the lowest quartile (ORQ4vsQ1 = 0.62; 95% CI = 0.51-0.76; p-trend < 0.001). No modification of the estimated ORs by sex, breakfast time, and number of eating occasions per day were observed. CONCLUSION: Our results suggest that higher breakfast size is associated with lower prevalence of MetS in Spanish adults, supporting the importance of a high energy breakfast. Further prospective studies are necessary to confirm these findings.

A review of the use of Duddingtonia flagrans as a biological controller of strongylid nematodes in horses.

In horses, the nematodes of the Strongylidae family are the most important due to their prevalence and pathogenicity. Sanitary plans include parasite control based on chemical anthelmintics. Among these, the benzimidazole compounds have been used since the 1960s to control the nematode Strongylus vulgaris. Its inappropriate use resulted in the development of resistance in parasites with a shorter biological cycle, such as the small strongyles. Currently, the genera that make up this group show widespread resistance to all chemical treatments available in veterinary medicine, except for macrocyclic lactones, where less effective action has been detected. The need to find alternative routes for its control is recognized. International organizations and markets are increasingly restrictive in the allowed levels of drugs in products of animal origin, so one of the drawbacks is the permanence of chemical compounds in tissues. Therefore, other tools not chemically based are proposed, such as the biological control of gastrointestinal nematodes. Various research groups around the world have carried out tests on the control capacity of the nematophagous fungus Duddingtonia flagrans against this group of parasites. The objective of this review is to compile the different tests that are available on biological control in this species, in in vivo and in vitro tests, and the possible incorporation of this tool as an alternative method of antiparasitic control in an integrated control scheme of parasites.

Supplementation of Infant Formula and Neurodevelopmental Outcomes: a Systematic Review.

PURPOSE OF THE REVIEW: The aim is to examine data from clinical trials and prospective longitudinal studies that evaluate the effect of infant formula supplements on the cognitive function of children. RECENT FINDINGS: A total of 300 articles from 2000 to 2021 were selected. The most researched IF supplements were initially long-chain polyunsaturated fatty acids (LC-PUFA), some proteins and, recently, milk fat globule membrane (MFGM). Supplementation of IF with LC-PUFA led to some positive effects on specific cognitive functions or no effect; however, there was no consistent benefit for cognitive function. Modifying the amount of proteins did not affect the children's neuropsychological tests. Supplementation of IF with MFGM and its components had beneficial effects on child cognitive development in the short term, but no effect was observed in the long term. Further studies are needed to confirm the safety of supplementation on the development of cognitive function in children fed with infant formula.

In vitro efficacy of different concentrations of Duddingtonia flagrans on varying egg densities of gastrointestinal nematodes of cattle.

The nematophagous fungus Duddingtonia flagrans, used for the biological control of gastrointestinal nematodes in livestock, is fed to infected animals so its chlamydospores and the parasite eggs are voided together with faeces where the fungus preys on nematode larvae, thus reducing pasture infectivity. The number of chlamydospores needed for the fungus to be efficient in the presence of a wide range in numbers of parasitic eggs is largely unknown and a matter of discussion. The aim of this study was to determine the fungal efficacy of four different chlamydospore concentrations against three different levels of cattle faecal egg counts. Fungal concentrations of 11000, 6250, 3000 and 1000 chlamydospores/gram of faeces (cpg) were added to cultures containing 840, 480 or 100 eggs/gram of faeces (epg). After 14 days of incubation, the efficacy of D. flagrans, in decreasing order of chlamydospore concentrations, ranged from 100% (P < 0.0001) to 77% (P > 0.0999) in the 100 epg groups; 100% (P < 0.0001) to 92% (P = 0.4625) in the 480 epg groups and 100% (P < 0.0001) to 96% (P = 0.7081) in the 840 epg groups. The results indicate that the numbers of eggs in cattle faeces were not a determining factor on the fungal efficacy against gastrointestinal nematodes.

The role of cellular senescence in tissue repair and regeneration.

The capacity to regenerate damaged or lost tissue varies widely along the animal kingdom and generally declines with aging of the organism. The gradual accumulation of senescent cells in tissues during aging has been causally involved in their reduced function at old age, and to be at the basis of age-related diseases. Recently, however, cellular senescence has been shown to play a positive role as a morphogenetic force modelling and promoting tissue development during embryogenesis, and to be responsible for tissue wound healing and repair. Work done on organismal models ranging from fish and amphibians, with extraordinary regenerative capacities, to mammals, with a more restricted regenerative potential, is shedding light on a novel and unexpected function of cellular senescence. In this review, we will analyze the senescence phenotype and how could it be contributing or restricting tissue regeneration.

Vitamin D status during pregnancy and offspring outcomes: a systematic review and meta-analysis of observational studies.

BACKGROUND/OBJECTIVES: Vitamin D deficiency during pregnancy may influence adverse outcomes in offspring. The aim of this systematic review and meta-analysis of observational studies was to assess the association between low prenatal concentrations of 25(OH)D (by using three different cut-off levels), preterm birth (PTB) and anthropometric and neurodevelopmental outcomes in offspring. SUBJECTS/METHODS: Studies reporting data on the association between maternal vitamin D concentrations and offspring outcomes identified through a systematic review of scientific literature published in PubMed/MEDLINE, Scopus and the Cochrane Library databases up to April 2017. RESULTS: We included 54 eligible studies. Vitamin D-deficient mothers (<30 nmol/L) had offspring with lower birthweight (MD -87.82 g; 95% CI -119.73, -55.91 g), head circumference (MD -0.19 cm; 95% CI -0.32, -0.06 cm) and a higher risk of small for gestational age (SGA) infants and PTB (OR 1.59; 95% CI 1.24, 2.03) compared to mothers with concentrations ≥30 nmol/L. Vitamin D insufficiency (<50 nmol/L) was associated with a higher risk of SGA and PTB (OR 1.43; 95% CI 1.08, 1.91 and OR 1.28; 95% CI 1.08, 1.52, respectively). Concentrations of 25(OH)D ≥75 nmol/L were not found to be associated with birthweight, SGA or PTB. Offspring of vitamin D-insufficient mothers had lower scores in mental (MD -1.12 points; 95% CI -1.82, -0.42 cm) and language developmental tests (MD -0.35 points; 95% CI -1.00, 0.31 cm). CONCLUSION: Maternal vitamin D deficiency is associated with offspring adverse anthropometric outcomes and PTB; insufficiency with a higher risk of SGA, PTB and adverse neurodevelopmental outcomes.

The development of cell senescence.

Cellular senescence was traditionally considered a stress response that protected the organism by limiting the proliferation of damaged and unwanted cells. However, the recent identification of developmentally-programmed cellular senescence during embryo development has changed our view of the process. There are now a number of examples of developmental senescence in evolutionary distant organisms ranging from mammals to fish, showing senescence at various sites during specific time windows of development. Developmental senescence shares many features with stress-induced senescence but also present some specific characteristics. The different examples of developmental senescence provide evidence of the diverse functions contributed by senescence and represent an opportunity to learn more about this process. Also, the existence of senescence during embryogenesis opens the possibility of identifying human developmental syndromes caused by alterations in this response. Studying in more detail this process will expand our understanding of cellular senescence and could offer new insights into the cause of human pathologies.

Tuning Oxygen Vacancy Diffusion through Strain in SrTiO3 Thin Films.

Understanding diffusion of oxygen vacancies in oxides under different external stimuli is crucial for the design of ion-based electronic devices, improvement of catalytic performance, and so forth. In this manuscript, using an external electric field produced by an atomic force microscopy tip, we obtain the room-temperature diffusion coefficient of oxygen-vacancies in thin films of SrTiO3 under compressive/tensile epitaxial strain. Tensile strain produces a substantial increase of the diffusion coefficient, facilitating the mobility of vacancies through the film. Additionally, the effect of tip bias, pulse time, and temperature on the local concentration of vacancies is investigated. These are important parameters of control in the production and stabilization of nonvolatile states in ion-based devices. Our findings show the key role played by strain for the control of oxygen vacancy migration in thin-film oxides.

Predatory effect of Duddingtonia flagrans on infective larvae of gastro-intestinal parasites under sunny and shaded conditions.

Duddingtonia flagrans is a natural strain of Nematophagous-Fungi isolated around the world. It has demonstrated efficacy and ease of use in laboratory as well as in field conditions. The fungus contributes to the prophylactic control of the worms by reducing the number of L3 on pasture. The aims of this study were to test and analyze the predatory effect of D. flagrans under sunny and shaded conditions on the L3 in the faeces, and to verify the reduction of translation to pasture during summer and winter seasons. Faecal Mass Units (FMUs) were assigned to two treated groups (groups treated with D. flagrans chlamydospores, TG) and two untreated groups (without D. flagrans chlamydospores, UG), in summer and winter, under sunny and shaded conditions. FMUs and herbage samples were taken for parasitological workup. Predatory activity of D. flagrans was evident under both conditions for the summer experiment but was not manifest for the winter experiment. In summer, an interaction between sunny and shaded conditions and predatory activity of D. flagrans was found. Environmental conditions on predatory activity should be considered when designing strategies for the implementation of D. flagrans in grazing systems to smooth the infectivity curve of L3.

Ivermectin dissipation and movement from feces to soil under field conditions.

The aim of this work was to evaluate the fate of ivermectin (IVM) at two concentrations in cattle feces and its movement to the nearby soil and plants. Feces were spiked with IVM at two levels: 3000 ng g-1 (high group, HG) and 300 ng g-1 (low group, LG). Artificial dung pats were prepared and deposited in an experimental field area. Feces and underlying soil were sampled up to 60 days post-deposition (dpd). As an additional analysis, grasses growing around the pats were sampled at 30 and 60 dpd. Ivermectin concentrations in all matrices were determined by HPLC. Mean IVM fecal concentrations were in the range between 3901.9 ng g-1 and 2419.2 ng g-1 (high group) and 375.3 ng g-1 and 177.49 ng g-1 (low group). Mean times for 50% and 90% dissipation were 88.23 and 293.03 days (HG) and 39.1 and 129.9 days (LG). Soil concentrations ranged from 26.1 ng g-1 to 71.1 ng g-1 (HG) and 3.4 to 5.9 ng g-1 (LG); in plants, concentrations were between 71.4 and 380.8 ng g-1 and 5.40 and 51.8 ng g-1 in HG and LG, respectively. These results confirm that IVM moves from feces to the underlying soil as well as to nearby plants. The potential risk of detrimental effects on soil organisms and the impact on herbivorous animals should be further evaluated.

Effects of prenatal iron status on child neurodevelopment and behavior: A systematic review.

Iron deficiency and iron-deficiency anemia are the main worldwide nutritional disorders. A good level of prenatal iron is essential for the correct child neurodevelopment but this association has been poorly investigated. To gather the scientific evidence on the relation between prenatal iron status and child neurodevelopment. To emphasize the importance of personalize the dose and type of supplementation. Wide search strategy was performed in electronic databases for English language articles with no limitations as regards the language or date of publication. Additional studies were selected by hand search. The inclusion criteria were pregnant women without high-risk pregnancy and their children as study population and neurodevelopment as the main outcome. Six RCTs and 13 observational studies were included. The majority concluded that deficit or excess iron during pregnancy injures the mental and psychomotor development of child. Other authors found no association of low iron level with troubles in neurodevelopment, recommended multi-micronutrients instead of iron alone and/or showed inconsistent results. Both iron deficiency as its excess are harmful for the child neurodevelopment. The prenatal iron supplementation should be adjusted for each woman, taking into account the iron stores, some genetic mutation and other health habits.

Nematophagous fungi from decomposing cattle faeces in Argentina / Hongos nematófagos en heces bovinas en descomposición en Argentina

Background. Biological control of gastrointestinal nematodes of ruminants by use of nematophagous fungi would become part of any livestock parasite integral control system. Identifying autochthonous species that could then be selected for mass production is an important phase in the practical use of biological control. Aims. To search for nematophagous fungi with potential use as biological control agents against gastrointestinal nematodes in Argentina. Methods. Decomposing cattle faeces sampled in different locations were incubated in water agar 2% with Panagrellus sp. The developed nematophagous fungi were transferred to new water agar 2% plates and then to corn meal agar plates in order to carry out their identification. Fungal diversity and richness were also assessed. Results. Seventeen species from nine genera of nematophagous fungi were found. Twelve species were nematode-trapping fungi and three species plus two fungi identified to genus level corresponded to endoparasitic fungi. Arthrobotrys conoides, Arthrobotrys oligospora, Duddingtonia flagrans, Monacrosporium doedycoides, Arthrobotrys robusta and Drechmeria coniospora were the most frequently isolated species overall in the whole study (6.6%, 5.7%, 5.7%, 5.7%, 4.7% and 4.7%, respectively) although other species were more frequently recorded at local levels such as Arthrobotrys pyriformis (18.8%). Only A. conoides has been previously isolated from ruminant faecal samples in Argentina. Five nematode-trapping fungal species are mentioned for the first time in the Americas. Conclusions. D. flagrans and A. conoides, both identified in the present study, are among the most promising ones as biological control agents against gastrointestinal nematodes of ruminants (AU)

Antecedentes. El control biológico de los nematodos gastrointestinales de los rumiantes mediante hongos nematófagos es una herramienta a considerar en los sistemas integrados de control parasitario del ganado. La identificación de las especies autóctonas de estos hongos que puedan ser seleccionadas para producción masiva es de gran importancia en el uso práctico del control biológico. Objetivos. Llevar a cabo una búsqueda de hongos nematófagos de uso potencial como agentes de control biológico contra nematodos gastrointestinales en Argentina. Métodos. Se trabajó con muestras de heces bovinas en descomposición obtenidas en diferentes lugares. Las heces se incubaron en agar agua 2% con Panagrellus sp. Los hongos nematófagos desarrollados se transfirieron a nuevas placas con agar agua 2% y luego a placas con agar harina de maíz para su identificación. También se estableció la diversidad y riqueza fúngicas. Resultados. Se aislaron diecisiete especies de hongos nematófagos, comprendidas en nueve géneros. Doce resultaron ser hongos depredadores, mientras que otras tres especies y dos hongos identificados hasta género eran hongos endoparásitos. Arthrobotrys conoides, Arthrobotrys oligospora, Duddingtonia flagrans, Monacrosporium doedycoides, Arthrobotrys robusta y Drechmeria coniospora fueron las especies más aisladas más en todo el estudio (6.6%, 5.7%, 5.7%, 5.7%, 4.7% y 4.7%, respectivamente), aunque otras especies aparecieron más frecuentemente de manera local, como Arthrobotrys pyriformis (18.8%). Solamente Arthrobotrys conoides se había aislado previamente en Argentina a partir de heces bovinas. Cinco especies depredadoras se mencionan por primera vez en toda América. Conclusiones. D. flagrans y A. conoides, dos de las especies aisladas en el presente estudio, se encuentran entre las más prometedoras como agentes de control biológico de nematodos gastrointestinales de rumiantes (AU)

Nematophagous fungi from decomposing cattle faeces in Argentina.

BACKGROUND: Biological control of gastrointestinal nematodes of ruminants by use of nematophagous fungi would become part of any livestock parasite integral control system. Identifying autochthonous species that could then be selected for mass production is an important phase in the practical use of biological control. AIMS: To search for nematophagous fungi with potential use as biological control agents against gastrointestinal nematodes in Argentina. METHODS: Decomposing cattle faeces sampled in different locations were incubated in water agar 2% with Panagrellus sp. The developed nematophagous fungi were transferred to new water agar 2% plates and then to corn meal agar plates in order to carry out their identification. Fungal diversity and richness were also assessed. RESULTS: Seventeen species from nine genera of nematophagous fungi were found. Twelve species were nematode-trapping fungi and three species plus two fungi identified to genus level corresponded to endoparasitic fungi. Arthrobotrys conoides, Arthrobotrys oligospora, Duddingtonia flagrans, Monacrosporium doedycoides, Arthrobotrys robusta and Drechmeria coniospora were the most frequently isolated species overall in the whole study (6.6%, 5.7%, 5.7%, 5.7%, 4.7% and 4.7%, respectively) although other species were more frequently recorded at local levels such as Arthrobotrys pyriformis (18.8%). Only A. conoides has been previously isolated from ruminant faecal samples in Argentina. Five nematode-trapping fungal species are mentioned for the first time in the Americas CONCLUSIONS: D. flagrans and A. conoides, both identified in the present study, are among the most promising ones as biological control agents against gastrointestinal nematodes of ruminants.

Electroplating and magnetostructural characterization of multisegmented Co54Ni46/Co85Ni15 nanowires from single electrochemical bath in anodic alumina templates.

Highly hexagonally ordered hard anodic aluminum oxide membranes, which have been modified by a thin cover layer of SiO2 deposited by atomic layer deposition method, were used as templates for the synthesis of electrodeposited magnetic Co-Ni nanowire arrays having diameters of around 180 to 200 nm and made of tens of segments with alternating compositions of Co54Ni46 and Co85Ni15. Each Co-Ni single segment has a mean length of around 290 nm for the Co54Ni46 alloy, whereas the length of the Co85Ni15 segments was around 430 nm. The composition and crystalline structure of each Co-Ni nanowire segment were determined by transmission electron microscopy and selected area electron diffraction techniques. The employed single-bath electrochemical nanowire growth method allows for tuning both the composition and crystalline structure of each individual Co-Ni segment. The room temperature magnetic behavior of the multisegmented Co-Ni nanowire arrays is also studied and correlated with their structural and morphological properties.

Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media.

The large-scale production of nematophagous fungi as agents of biological control is one of the main challenges to be commercially used. In order to improve growth of microorganism in a culture medium, the addition of growth inducer is common. At the moment, the action of their addition in the mycelia growth and sporulation rate of nematophagous fungi is not known. The purpose of this trial was to evaluate the sporulation rate of Duddingtonia flagrans by adding two growth inducers, meso-inositol and Tween 80, both at 0.5 % in a traditional culture medium Sabouraud glucose agar (SGA) and also in a traditional culture medium enriched with wheat flour and milk powder. From a traditional sterile culture of D. flagrans, four groups were made: SGA; Sabouraud glucose agar-meso-inositol 0.5 %; Sabouraud glucose agar-Tween 80 0.5 %; and Sabouraud glucose agar-enriched (SGA-E). These media were placed at a constant temperature of 27 °C for 4 weeks. Following this, chlamydospores were gently rinsed off with sterile water and counted using a Neubauer haematocytometer to estimate the number of chlamydospores per millilitre of water. The addition of meso-inositol 0.5 % to SGA promoted a significant increase (p < 0.05) in chlamydospore production obtaining an average of 51,715,000 chlamydospores per Petri dish. The highest chlamydospore concentration was observed in the SGA-E in comparison with SGA (p < 0.01) obtaining an average of 208,760,000 chlamydospores. The aim of this study was to obtain basic knowledge regarding the effect of enriched culture medium and growth-inducing meso-inositol and Tween 80 on mycelial growth and production of chlamydospores.

Hongos nematófagos utilizados para el control biológico de nematodos gastrointestinales en el ganado y sus formas de administración / Nematophagous fungi used for the biological control of gastrointestinal nematodes in livestock and administration routes

En la actualidad el control de los nematodos gastrointestinales se realiza casi exclusivamente con moléculas antihelmínticas. La inadecuada utilización ha generado resistencia en algunos géneros parasitarios. Sumado a la preocupación de consumir alimentos inocuos en pro de la salud humana, se ha estimulado el desarrollo de otros métodos de control para las parasitosis gastrointestinales, tales como el control biológico, basado en el uso de enemigos naturales de las larvas en el medio ambiente. Entre los enemigos naturales de las larvas de nematodos gastrointestinales el más frecuentemente utilizado es el hongo Duddingtonia flagrans. Este tiene la capacidad de reducir el número de larvas de nematodos en materia fecal, y sus clamidosporas, así como de atravesar el tracto gastrointestinal y mantener su capacidad germinativa, facilitando así la posibilidad de desarrollar diferentes formas de administración. En esta revisión se mencionan las especies de hongos utilizadas hasta el momento y se discuten las distintas formas de administración de hongos nematófagos ensayadas hasta el momento(AU)

The control of gastrointestinal nematodes relies at present mostly on antihelmintic treatments using synthetic molecules. This approach, however, has led to the appearance of resistance to some types of antihelmintics which, together with the need to cut down on the use of chemicals, has fostered the development of other control methods, such as biological control, which is the use of living organisms that are naturally antagonistic to an unwanted species. Among the natural enemies of nematode parasitic larvae is the microfungus Duddingtonia flagrans. Research has shown the ability of this fungus to reduce the number of nematode larvae in faeces, the ability of its chlamydospores to survive the passage through the gastrointestinal tract of livestock and, moreover, to keep its germinative ability, thus facilitating the development of formulations. The present review looks at the species currently used and the different ways of administering already tested nematophagous fungi(AU)