RESUMO
We studied a new method of treatment of amyotrophic lateral sclerosis with autologous mesenchymal stem cells. Autologous mesenchymal stem cells were injected intravenously (intact cells) or via lumbar puncture (cells committed to neuronal differentiation). Evaluation of the results of cell therapy after 12-month follow-up revealed slowing down of the disease progression in 10 patients in comparison with the control group consisting of 15 patients. The cell therapy was safe for the patients.
Assuntos
Esclerose Lateral Amiotrófica/terapia , Transplante de Células-Tronco Mesenquimais , Adulto , Idoso , Esclerose Lateral Amiotrófica/metabolismo , Esclerose Lateral Amiotrófica/patologia , Diferenciação Celular , Células Cultivadas , Feminino , Humanos , Masculino , Células-Tronco Mesenquimais/enzimologia , Pessoa de Meia-Idade , Nestina/metabolismo , Fosfopiruvato Hidratase/metabolismo , República de Belarus , Transplante Autólogo , Resultado do TratamentoRESUMO
We studied the effect of platelet releasate on osteogenic differentiation of human mesenchymal BM stem cells. Histological staining and reverse transcription PCR analysis showed that addition of platelet releasate to osteogenic differentiation medium stimulated the formation of calcium ossificates and alkaline phosphatase and increased expression of osteopontin and alkaline phosphatase genes.
Assuntos
Plaquetas/fisiologia , Diferenciação Celular , Células-Tronco Mesenquimais/fisiologia , Osteogênese , Fosfatase Alcalina/genética , Fosfatase Alcalina/metabolismo , Extratos Celulares , Células Cultivadas , Indução Enzimática , Humanos , Osteopontina/genética , Osteopontina/metabolismoRESUMO
Studies on the immediate and long-term effects of radiation on the B-system immunity of children who were affected by radiation after the Chernobyl disaster (from 1986-1992) are summarized in this paper. Complete clinical and immunological examination of more than 6000 children have been carried out. The dynamics of the immune system, with ongoing reactions of cell proliferation and differentiation, gene amplification, transcription, translation, biosynthesis and switching production of isotypes and subclasses of immunoglobulins, as well as specific and nonspecific (natural) antibodies, make it highly susceptible to the action of radiation in addition to other ecological factors. B-system of immunity (B-cel level, concentration of immunoglobulins-M, G, A, E; subclasses of IgG (IgG1-IgG4) in the serum and saliva, and the level of nonspecific heterophilic autoantibodies (RF, antithyroglobulin) were investigated in children of differing ages and sex living in the territories of the Republic of Belarus contaminated with radionuclides. Research showed decreased levels of B-cell and IgM and IgG isotopes 40-50 days after the disaster and increased levels of IgA immunoglobulins at that time. Long-term effects of low doses of radiation showed increased concentrations of IgM and IgG, correlating changes in the B-system of immunity with the level of 137Cs contamination in the territory of residence and also with the amount of 137Cs found in the children.
Assuntos
Imunoglobulinas/biossíntese , Imunoglobulinas/efeitos da radiação , Centrais Elétricas , Liberação Nociva de Radioativos , Adolescente , Radioisótopos de Césio/metabolismo , Criança , Humanos , Sistema Imunitário/metabolismo , Imunidade/efeitos da radiação , Iodo/metabolismo , Radioisótopos do Iodo , Glândula Tireoide/metabolismo , Fatores de Tempo , UcrâniaRESUMO
Experiments on calves undergoing implantation of artificial hearts (Soviet models) under extracorporeal circulation were made to examine platelet adhesion and aggregation. It was demonstrated that the increase in platelet adhesion and aggregation set in from the 2nd hour of the artificial heart work and lasted till the end of the experiments. It was discovered that platelet aggregation correlated well with fibrinogen concentration.
