[Effectiveness of measles immunoprophylaxis and the criteria for assessing its possible elimination]. / Effektivnost' immunoprofilaktiki kori i kriterii otsenki vozmozhnosti ee likvidatsii.

The study of measles morbidity in Donetsk Province in 1960-1984 and the study of antimeasles immunity in different groups of the population have shown that the problem of the liquidation of measles cannot be solved by immunizing the population in a single administration of the vaccine. To enhance the effectiveness of the immunoprophylaxis of measles and its influence on the epidemic process, a number of problems must be solved with the aim to improve the quality of the vaccine, especially its thermal stability, to establish the possibility of shifting the beginning of immunization from 15-17 months to 12 months of age, to increase the coverage of children with immunization against measles by decreasing the number of groundless exemptions from immunization and by immunizing children in risk groups according to individual schedules and dosage, to carry out selectively the booster immunization of persons who have lost their postvaccinal immunity, as revealed by laboratory test, or in whom such loss may be supposed, to introduce the objective method (indirect hemagglutination test) for controlling the state of immunity among different groups of the population into laboratory practice at sanitary and epidemiological stations. As to the possibility of the liquidation of measles, the statement of this problem is correct, but for its solution a complex of additional prophylactic and epidemic-control measures should be taken.

[Effect of immunization with neurospecific proteins and tubulin on learning in the rat]. / Vliianie na obuchenie krys immunizatsii neirospetsificheskimi belkami i tubulinom.

The paper deals with the effect of anticerebral antibodies on rats learning in T-shaped maze with food reinforcement. The rats of August line were immunized by neurospecific proteins 10-40-4 from the human and rat's brain, 14-3-2 and cerebral tubuline. Rats immunized by bovine serum albumine (BSA) served as a control of the influence of antibodies to noncerebral protein. Rats injected with Freund adjuvant and saline, served as control of Freund adjuvant action. At the time of antibodies formation (from the 7-th day after the last injection) the rats were trained in a T-shaped maze during 4 days. The acquisition of conditioned reactions was inhibited in rats immunized with neurospecific proteins 10-40-4 from the rats brain, 14-3-2 and cerebral tubuline. Antibodies to neurospecific protein 10-40-4 from the rats brain produced the greatest effect on the process of learning. They elicited a significant decrease of conditioning and an increase of the number of errors. Antibodies to the neurospecific protein 10-40-4 from the human brain and to the BSA, i.e. to proteins which are not present in the nervous tissue of the rats, did not affect the learning. The obtained results indirectly confirm the permeability for the antibodies of the blood-brain barrier in immunized animals.

[Immunoglobulins of classes G, A and M in the serum of schizophrenics]. / Immunoglobuliny klassov G, A i M v syvorotke krovi bol'nykh shizofreniei.

The levels of immunoglobulins, G, A and M in the blood serum were examined in 84 schizophrenic patients and 87 normal donors. The concentration of immunoglobulins G and M was elevated in patients in the period of exacerbation as compared to normal subjects. The levels of these immunoglobulins in patients on psychotropic drugs as against normal subjects were unchanged. The concentration of immunoglobulins G and M depended on the sex, clinical state and the effect of psychotropic therapy, that of immunoglobulin A on the age and duration of the manifest period.

[Beta-endorphin concentration in the cerebrospinal fluid of schizophrenics]. / Soderzhanie beta-éndorfina v likvore bol'nykh shizofreniei.

beta-endorphin concentration in cerebrospinal fluid (CSF) in 21 schizophrenic and 15 neurological patients was determined by radioimmune assay. On the basis of the obtained data a correlation between the change of beta-endorphin level in CSF of schizophrenics and that of the clinical state of these patients was assumed as a result of a short-term discontinuance of the treatment. It was established that the reduction of beta-endorphin level in CSF of schizophrenics correlates with the intensification of hallucinatory-delusional disorders.

[Organ specific water-soluble cerebral cortex proteins; possible participation in physiological functions and pathologic manifestations]. / Organospetsificheskie vodorastvorimye belki kory golovnogo mozga; vozmozhnoe uchastie v fiziologicheskikh funktsiiakh i patologicheskikh proiavleniiakh.

The spectre of active antigens in brain is studied. The presence of 3 protein and 3 glycoprotein brain specific antigens is demonstrated. The possible participation of brain specific antigens in increased antibody formation in schizophrenics, multiple sclerosis and lateral ammiotrophic sclerosis is studied. The data obtained indicate the role of brain specific antigens to autoimmunity in those patients. The possible participation of one of the specific antigens of the brain, sialoglycoprotein GP-350, in physiological processes connected with the mechanism of memory is also studied. In experiments of inbred trained rats an activation of the synthesis of GP-350 is observed.

[Use of affinity chromatography for isolation of antibodies to human brain-specific antigens and several findings on the characteristics of these antigens]. / Ispol'zovanie metoda affinnoi khromatografii dlia vydeleniia antitel k mozgospetsificheskim antigenam cheloveka i nekotorye danny k kharakteristikeétikh antigenov.

With the aid of affinity chromatography on sepharose-4B, activized by CNBr from the blood serum of rabbits who were previously immunized by general phospate protein extract of the human brain, the author identified antibodies to brainspecific antigens. The specificity of the directness of the obtained antibodies was proved by the reaction of double diffusion in agar gel. With the aid of the same antigens by the immunoelectrophoresis method two brainspecific antigens of a glycoproteid nature with an electrophoretic mobility alpha1 and alpha2-globulins were detected. A processing of the same immunophoregrams by labeled 125i antibodies to rabbit gamma-globulins permitted to find another brainspecific antigen with a mobility beta2-gamma-globulin.

[Serum antibodies to cytoplasmic antigens of the human brain in patients with multiple sclerosis and amyotropic lateral sclerosis]. / Syvorotochnye antitela k tsitoplazmaticheskim antigenam mozga cheloveka u bol'nykh rasseiannym sklerozom i bokovym amiotroficheskim sklerozom

In order to eliminate antibodies to water soluble proteins of the human brain which were preliminary divided into 10 fractions of DEAE-cellulose, the authors studied the serum of 30 patients with disseminated sclerosis and 30 patients with lateral amyotrophical sclerosis and determined their antithymic serum activity. The antibides were detected only to protein fractions 1,2 and 10. There was a tendency towards a more frequent detection of antibodies with an increase of the severity of the disease. It was not possible to determine antibodies with an increase of the severity of the disease. It was not possible to determine any correlation between the amount of antibodies and such clinical indices as the degree of severity, duration, form of the disorder, age and sex of the patients. The cytotoxic index of the patients was significantly higher than that in normals. The authors also found a correlation between the antibodies in the serum cytoplasmatic brain proteins and antithymic activity of the same sera.

[Antigenic composition of protein fractions of the human cerebral cortex extracted by chromatography on DEAE-cellulose]. / Antigennyi sostav belkovykh fraktsii kory golovnogo mozga cheloveka, poluchennykh khromatografiei na DEAE-tselliuloze

In fractionating a phosphate protein extract from the human brain cortex on DEAE-cellulose 10 fractions of the basic acid proteins were received. Analyses of these fractions were conducted by the method of disc electrophoresis in polyacridamide gel with dodecylsulphate Na and a subsequent separate staining of the desitograms of proteins and glycoproteins, precipitation in agar gel, immunoelectrophoresis and autoimmunography with the use of labeled I125 antibodies to rabbit gamma-globulin. In the first 2 basic fractions there were brain specific antigens with electrophoretic mobility of the beta2--gamma globulin. In the first acid fraction--2 antigens: alpha1-globulin and alpha2-globulin of a glycoprotein nature. In subsequent acid fractions--one antigen with a mobility of alpha1-globulin. A study of the antigen activity of these fractions in a complement fixation test on cold with the serum of schizophrenic patients and disseminated sclerosis depicted differences in the activity of the basic and acid fractions.