[The topographic characteristics and changes in the ontogeny of light-chain myosin in the human myocardium]. / Izuchenie topograficheskikh osobennostei i izmenenii v ontogeneze legkikh tsepei miozina v miokarde cheloveka.

Pattern of myosin light chain in human atrium and ventricles was studied using two-dimensional electrophoresis. Minor fraction was found in ventricles and atria of adult man that coincided in molecular weight, isoelectric point and staining specificity with fetal myosin light chain. The 23 kDa and 24 kDa fractions of auricles were not detected in ventricles.

[Two-dimensional map of membrane proteins from human erythrocytes]. / Dvumernaia karta membrannykh belkov éritrotsitov cheloveka.

Human erythrocyte membrane proteins were analyzed by a modified two-dimensional electrophoresis performed according to O'Farrell. This method was used to construct a two-dimensional map of human erythrocyte membrane proteins. The map plotted in the coordinates "relative molecular mass versus relative electrophoretic mobility during IEF" was used for the characterization of 189 proteins. The position of major membrane proteins in the map was determined on the basis of their Mr, pI as well as literature data. Carboanhydrase was positioned by coelectrophoresis. A comparative analysis of erythrocyte membrane and cytosol preparations by two-dimensional protein mapping revealed that some of erythrocyte proteins have dual localization.

[Analysis of the results of two-dimensional electrophoretic fractionation of proteins using a system of digital processing of video information]. / Analiz rezul'tatov dvumernogo élektroforeticheskogo fraktsionirovaniia belkov na komplekse tsifrovoi obrabotki videoinformatsii (SVIT).

Possible solution of four main problem in analysis of two-dimensional electrophoregrams using the complex set for processing of video-information SVIT is considered: I. a. potentiality for evaluation of correlation between integral optic density of spots on gel plates and amount of protein fractionated, 2. qualitative comparison of similar spots from various electrophoregrams, 3. the problem of overlapping spots, where the plane pictures of spots were transformed into three-dimensional system of peaks, 4. use of bench-marks for geometric transformations and production of unified pictures. The complex set or its modifications were able to settle these problems.