[Improvement in the laboratory methods of assessing the safety of the of the pertussis component of DPT vaccine adsorbed]. / Usovershenstvovanie laboratornykh metodov otsenki bezvrednosti kokliushnogo komponenta AKDS-vaktsiny.

The mouse paw edema test and the tests for the determination of leukocytosis-stimulating and histamine-sensitizing activity are reproducible if standard samples are used and the properties under test are evaluated with reference to the characteristics of standard samples. The standard character of the weight gain test on mice is also enhanced if all conditions of the test are standardized. The most accurate information on the toxicity of the pertussis component of adsorbed DPT vaccine can be obtained if a single human dose (0.5 ml) is injected to mice and the toxicity of the vaccine is estimated from the weight gain registered in the test mice and the control animals. The use of standard samples in each test makes it possible to observe the conditions for controlling the toxicity of the tested preparations.

[Comparative study of the immunoglobulins and specific antibodies in the blood of rabbits immunized with ADTP vaccine and ADT-anatoxin with normal and reduced antigen contents]. / Sravnitel'noe izuchenie immunoglobulinov i spetsificheskikh antitel v krovi krolikov, immunizirovannykh AKDS-vaktsinoi i ADS-anatoksinom obychnogo sostava i s umen'shennym soderzhaniem antigenov.

Adsorbed DPT vaccine and adsorbed DT toxoids with normal and reduced antigen content were used for the immunization of rabbits. The levels of IgM and IgG and the dynamics of antibodies to diphtheria and tetanus toxins and to Bordetella pertussis in the blood sera of the animals were studied in the postvaccinal period (on days 15 and 34). This study revealed that the reduction of the antigen content of adsorbed DT toxoid to 5 Lf of diphtheria toxoid and 5 binding units of tetanus toxoid did not decrease the capacity of the preparation for increasing the levels of IgG and IgM, antibodies to diphtheria and tetanus toxins in the sera of the rabbits. The reduced content of these toxoids in adsorbed DPT vaccine did not affect its capacity for inducing the enhanced synthesis of IgG, antibodies to diphtheria and tetanus toxins, while the production of IgM and IgA remained unchanged. At the same time an increase in the titers of antibodies to B. pertussis in the animals was less pronounced than that observed after the injection of commercial adsorbed DPT vaccine. Additional investigations are necessary in order to establish the protective potency of the pertussis component in adsorbed DPT vaccine with the reduced content of toxoids and to find out the optimum antigenic composition for this preparation.

[Biologic-economic study of processes for cultivating Clostridium perfringens. II. Comparison of the effectiveness of actual and predicted processes for cultivating microorganisms]. / Biolog-ékonomicheskoe izuchenie protsessov kul'tivirovaniia Clostridium perfringens. Soobshchenie II. Sravnenie éffektivnosti real'nykh i prognoziruemykh protssessov kul'tivirovaniia mikroorganizmov.

The authors analyzed the efficacy of actual and prognosticated processes of Cl. perfringens, type A, cultivation: periodic, semicontinuous, continuous, and combined monocyclic. Comparative study permitted to choose the economically optimal processes, without any expenditure for their realization. Monocyclic combined process which could provide a great amount of active toxin at short periods proved to be the most advantageous among the cultivation processes studied.