RESUMO
BACKGROUND: Volatile organic compounds (VOCs) are potential biomarkers for cancer detection in breath, but it is unclear if they reflect specific mutations. To test this, we have compared human bronchial epithelial cell (HBEC) cell lines carrying the KRAS(V12) mutation, knockdown of TP53 or both with parental HBEC cells. METHODS: VOC from headspace above cultured cells were collected by passive sampling and analysed by thermal desorption gas chromatography mass spectrometry (TD-GC-MS) or sensor array with discriminant factor analysis (DFA). RESULTS: In TD-GC-MS analysis, individual compounds had limited ability to discriminate between cell lines, but by applying DFA analysis combinations of 20 VOCs successfully discriminated between all cell types (accuracies 80-100%, with leave-one-out cross validation). Sensor array detection DFA demonstrated the ability to discriminate samples based on their cell type for all comparisons with accuracies varying between 77% and 93%. CONCLUSIONS: Our results demonstrate that minimal genetic changes in bronchial airway cells lead to detectable differences in levels of specific VOCs identified by TD-GC-MS or of patterns of VOCs identified by sensor array output. From the clinical aspect, these results suggest the possibility of breath analysis for detection of minimal genetic changes for earlier diagnosis or for genetic typing of lung cancers.
Assuntos
Células Epiteliais/metabolismo , Neoplasias Pulmonares/genética , Proteínas Proto-Oncogênicas/genética , Proteína Supressora de Tumor p53/genética , Compostos Orgânicos Voláteis/análise , Proteínas ras/genética , Ar/análise , Inteligência Artificial , Brônquios , Células Cultivadas , Análise Discriminante , Cromatografia Gasosa-Espectrometria de Massas , Técnicas de Silenciamento de Genes , Humanos , Análise em Microsséries , Mutação , Proteínas Proto-Oncogênicas p21(ras)RESUMO
Cyprinid herpesvirus 3 is a highly contagious and lethal virus that affects ornamental koi and common carp worldwide. However, it is not yet known whether other cyprinids are infected and/or harbor the virus. Here, we report that cultured cells derived from common carp, koi, silver carp and goldfish allow CyHV-3 propagation, while cyprinid cells derived from fathead minnow and non-cyprinid cells derived from the channel catfish ovary are resistant to CyHV-3 infection. Interestingly, the epithelioma papulosum Cyprini cells derived from Cyprinus carpio are restrictive to the virus. These results indicate that CyHV-3 is not restricted to common carp and koi, but other cyprinids are also vulnerable to the virus.
