RESUMO
BACKGROUND: For successful stem cell-based therapy, not only are alternative good cell sources needed but appropriate scaffolds are key factors. The main purpose of this study was to evaluate the multipotentiality of multilineage progenitor cells (MLPC) and assess the three-dimensional cultivation and chondrogenic differentiation of MLPC in atelocollagen gel for application of tissue-engineered cartilage constructs. METHODS: MLPC, human umbilical cord blood-derived clonal cell lines, from BioE Inc. were used. Immunophenotypes of MLPC were characterized using a fluorescence-activated cell sorter (FACS). In vitro differentiation potentials into osteogenic, adipogenic and chondrogenic lineages were examined. Differentiated cells were characterized by reverse transcriptase-polymerase chain reaction (RT-PCR), histologic and immunofluorescence staining. RESULTS Clonogenic MLPC maintained immunophenotypes with specific surface markers of mesenchymal stromal cells (MSC). The osteogenic and adipogenic potentials of MLPC were demonstrated by quantitative real-time PCR, alkaline phosphates activity and Oil Red O staining. Furthermore, MLPC were successfully differentiated into chondrocytes in atelocollagen gel, which was confirmed by RT-PCR and immunofluorescence staining for type II collagen protein. DISCUSSION: Whenever MSC are considered for the treatment of cartilage defects, a variety of scaffolds have been utilized as successful carriers for cell delivery. Our results suggest that MLPC can serve as an alternative source for stem cell-based therapy and transplantation. The chondrogenic potential of MLPC in atelocollagen could be suitable for cartilage tissue engineering.
Assuntos
Linhagem da Célula , Condrócitos/citologia , Condrogênese , Colágeno/metabolismo , Sangue Fetal/citologia , Células-Tronco/citologia , Adipogenia , Linhagem da Célula/efeitos dos fármacos , Células Cultivadas , Condrócitos/efeitos dos fármacos , Condrogênese/efeitos dos fármacos , Condrogênese/genética , Colágeno/farmacologia , Sangue Fetal/efeitos dos fármacos , Géis , Humanos , Imunofenotipagem , Osteogênese , Células-Tronco/efeitos dos fármacosAssuntos
Desoxiguanosina/análogos & derivados , Estresse Oxidativo , Gravidez/genética , Efeitos Tardios da Exposição Pré-Natal , Poluição por Fumaça de Tabaco/efeitos adversos , 8-Hidroxi-2'-Desoxiguanosina , Citocromo P-450 CYP1A1/genética , Citocromo P-450 CYP1A1/metabolismo , Citocromo P-450 CYP2E1/genética , Citocromo P-450 CYP2E1/metabolismo , Desoxiguanosina/urina , Feminino , Feto/efeitos dos fármacos , Feto/metabolismo , Genótipo , Glutationa Transferase/genética , Glutationa Transferase/metabolismo , Humanos , Inativação Metabólica , Recém-Nascido , Malondialdeído/urina , Hidrocarbonetos Policíclicos Aromáticos/farmacocinética , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Polimorfismo Genético , Gravidez/metabolismoAssuntos
Receptores da Transferrina/sangue , Adolescente , Adulto , Fatores Etários , Criança , Pré-Escolar , Humanos , Lactente , Recém-Nascido , Pessoa de Meia-Idade , Fatores SexuaisRESUMO
The upper normal limit of menstrual blood loss (MBL) associated with iron deficiency, and the change of MBL and length of time necessary for development of iron depletion after IUD insertion in 193 healthy Korean women were studied. The mean MBL was 33.4 +or- 23.4 (SD) ml and the variation of MBL in different marital, age, and parity groups showed no statistical significance except for the higher values in young married women. Serum ferritin concentration was markedly decreased in women showing MBL above 40 ml and the frequency of subjects with serum ferritin below anemia criteria were increased in women showing MBL above 50 ml. Other parameters for assessing the iron status showed no significant difference in a different MBL range. The mean MBL in women fitted with an Alza-T IPCS 52 was significantly decreased 3 months after insertion, but the increases in hemoglobin and serum ferritin levels were statistically significant at 12 months postinsertion. The mean MBL in other IUD groups was remarkably increased at 1 month in T-Cu 220C users and between months 1-12 in Lippes Loop D users. Significant decreases in serum ferritin levels were observed at 6 and 12 months in both groups.
