Physiological and electroencephalogram responses in goats subjected to pre-and during slaughter stress.

A comprehensive stress assessment is vital in understanding the impact of the pre-slaughter procedure on animal welfare. The transportation and handling process was commonly reported to cause stress in animals. This research utilises electroencephalography (EEG) as an alternative stress indicator to non-painful acute stress measurement. EEG has been proved to be instantaneous and sensitive with specific results. Therefore, this study was aimed to determine the stress level of goats subjected to two different transportation duration and the effect of lairage based on their EEG activities and blood parameters changes. Eighteen adult male goats were divided into two transportation stress groups based on the transport duration: the two-hour (TS2) and six-hour (TS6) groups. Then, each group was then again divided into three smaller groups according to the lairage duration, which was three-hour (L3), six-hour (L6), and overnight (L12) groups. Blood was sampled before transport, after transport, and during slaughter while EEG was recorded before transport, after transport, after lairage, and during slaughter. Results revealed that there was a significant decrease in beta wave activity compared to baseline in TS2 goats (P < 0.05) after transportation, whereas no significant difference was detected in the TS6 goats. At the same time, goats from the TS2 group showed increase in creatine kinase (CK) and lactate dehydrogenase (LDH) compared to that in TS6 goats. Together with the observed cortisol concentration, these findings showed that the TS6 goats were fully adapted to the transportation stress while the TS2 goats were still under stress. As for the lairage duration, it was observed that the TS2L3 goats showed lower EEG activities than the values obtained after two-hour transportation, while lower EEG activities were found from the TS6L6 goats after six-hour transportation. Therefore, it can be concluded that three-hour lairage was adequate to lower the impact of two hours transportation stress, whereas six-hour lairage was required to reduce the impact of six hours transportation stress. Finally, it was also found that the TS6L3, TS6L6, and TS6L12 groups took a long time to die after slaughter than the TS2L3, TS2L6, and TS2L12 goats based on the time their EEG activity reached isoelectric.

The Effects of Stocking Density and Distances on Electroencephalographic Changes and Cortisol as Welfare Indicators in Brahman Crossbred Cattle.

The objective of the current study was to investigate the effects of stocking density and distances on electroencephalographic changes and cortisol as welfare indicators in Brahman crossbred cattle. Sixty Brahman crossbred heifers were subjected to road transport from a cattle feedlot farm located in Universiti Putra Malaysia (UPM), Serdang to a commercial ruminant abattoir in Shah Alam, Selangor. Animals were assigned to long (850 km) and short (450 km) distances and high (600 sqm), medium (400 sqm), and low (200 sqm) stocking densities. Results revealed that the intensity of cortisol responses and EEG parameters (such as alpha <0.001, beta < 0.001, delta < 0.001, theta < 0.001, MF < 0.001 and Ptot < 0.001) increased significantly. Long-distance transport also resulted in significantly more intense (<0.001) responses to nociception during slaughter than animals that had been transported over a shorter distance, as indicated by EEG and cortisol.

Effects of Stocking and Transport Conditions on Physicochemical Properties of Meat and Acute-Phase Proteins in Cattle.

This study's objective was to evaluate the effects of distance and stocking density on physicochemical properties and oxidative stability of meat and acute-phase proteins in Brahman crossbred cattle transported by road under hot and humid tropical conditions. Sixty Brahman crossbred heifers were subjected to road transport from a cattle feedlot farm located in Universiti Putra Malaysia (UPM), Serdang, to a commercial ruminant abattoir in Shah Alam, Selangor. Animals were assigned to long and short distances and high, medium, and low stocking densities. The results revealed that the intensity of response significantly increased in meat samples from animals subjected to long-distance transportation and higher stocking density. Alpha-1-acid glycoprotein and serum amyloid-A values increased considerably and were different from the baseline values recorded at preload. In conclusion, the current results revealed that the color, pH, shear force values, water holding capacity (WHC), glycogen level, and malondilaldehyde assay (MDA) concentrations in meat and acute-phase proteins (APP) were affected by both distances and stocking densities, as evidenced by the significant changes recorded from the parameters above.

The Effects of Sea and Road Transport on Physiological and Electroencephalographic Responses in Brahman Crossbred Heifers.

The objective of the current study was to evaluate the effects of sea and road transport on the acute phase proteins (APP), cortisol, metabolic, haematological and electroencephalographic (EEG) responses of Brahman crossbred heifers. Sixty Brahman crossbred heifers were subjected to 14 d of transportation by sea from Darwin Port, Australia, to Pasir Gudang Port, Johor, Malaysia, and 330 km of road transportation. Results revealed that the intensity of response for most blood biochemical parameters increased significantly and were different from the baseline values taken while the animals were in Darwin Port, Australia. Haematological results obtained also revealed a significant increase and were different from the baseline values. Cortisol and APP (bovine alpha 1-acid glycoprotein and serum amyloid-A) values increased significantly and were different from the baseline values. Haematological parameters, APP, cortisol and EEG data (alpha, beta, delta and theta waves, total power and median frequency) decreased significantly following 4 and 7 days post-transport, suggesting a recovery of the animals from the stressfulness of transport. In conclusion, the current results revealed that the concentrations of biochemical and haematological parameters, cortisol, APP and EEG data were affected by both sea and road transport as evidenced by the significant changes recorded from the parameters above.

Effects of Ultrasound Assisted Extraction in Conjugation with Aid of Actinidin on the Molecular and Physicochemical Properties of Bovine Hide Gelatin.

Actinidin was used to pretreat the bovine hide and ultrasonic wave (53 kHz and 500 W) was used for the time durations of 2, 4 and 6 h at 60 °C to extract gelatin samples (UA2, UA4 and UA6, respectively). Control (UAC) gelatin was extracted using ultrasound for 6 h at 60 °C without enzyme pretreatment. There was significant (p < 0.05) increase in gelatin yield as the time duration of ultrasound treatment increased with UA6 giving the highest yield of 19.65%. Gel strength and viscosity of UAC and UA6 extracted gelatin samples were 627.53 and 502.16 g and 16.33 and 15.60 mPa.s, respectively. Longer duration of ultrasound treatment increased amino acids content of the extracted gelatin and UAC exhibited the highest content of amino acids. Progressive degradation of polypeptide chains was observed in the protein pattern of the extracted gelatin as the time duration of ultrasound extraction increased. Fourier transform infrared (FTIR) spectroscopy depicted loss of molecular order and degradation in UA6. Scanning electron microscopy (SEM) revealed protein aggregation and network formation in the gelatin samples with increasing time of ultrasound treatment. The study indicated that ultrasound assisted gelatin extraction using actinidin exhibited high yield with good quality gelatin.