RESUMO
PURPOSE: To investigate the effects of Arg-Gly-Asp (RGD) peptides on cells derived from the human eye. METHODS: SV40-transformed human corneal epithelial cells (SCE), SV40-transformed human lens epithelial cells (SLE), normal human keratocytes (NK), and human retinal pigment epithelial cells stably expressing human telomerase reverse transcriptase (TRPE) were used. A human corneal cDNA library was screened to isolate the human homologue of p130 Crk-associated substrate (Cas). Next, after these cells were attached to the substratum, RGD-containing soluble tetrapeptides Arg-Gly-Asp-Ser (RGDS) were added to the culture medium and morphological changes were observed. RESULTS: A clone, H-2, consisting of 3228 nucleotides, with a long open reading frame (870 amino acid residues) was isolated, and determined to be the human homologue of Cas. After addition of the RGDS peptides, both SLE and TRPE detached from the plastic culture plate, but SCE and NK did not. Detached SLE and TRPE showed decreased levels of tyrosine phosphorylation in Cas. CONCLUSIONS: These results indicated that the signaling pathway through Cas played an important role in epithelial cell adhesion in the eye.
Assuntos
Córnea/efeitos dos fármacos , Epitélio Corneano/efeitos dos fármacos , Cristalino/efeitos dos fármacos , Oligopeptídeos/farmacologia , Epitélio Pigmentado Ocular/efeitos dos fármacos , Sequência de Aminoácidos/genética , Sequência de Bases/genética , Adesão Celular/efeitos dos fármacos , Linhagem Celular Transformada , Proteína de Suscetibilidade a Apoptose Celular/genética , Proteína de Suscetibilidade a Apoptose Celular/metabolismo , Córnea/citologia , Proteínas de Ligação a DNA , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/fisiologia , Epitélio Corneano/citologia , Epitélio Corneano/fisiologia , Humanos , Integrinas/metabolismo , Cristalino/citologia , Cristalino/fisiologia , Dados de Sequência Molecular , Oligopeptídeos/química , Fragmentos de Peptídeos/farmacologia , Fosforilação/efeitos dos fármacos , Epitélio Pigmentado Ocular/citologia , Epitélio Pigmentado Ocular/enzimologia , Epitélio Pigmentado Ocular/fisiologia , Telomerase/metabolismo , Tirosina/metabolismoRESUMO
PURPOSE: To evaluate the rapid effects of pulse corticosteroid therapy on the serous retinal detachment found at the acute phase of Vogt-Koyanagi-Harada disease. DESIGN: Interventional case series. METHODS: Nine Japanese patients determined to be at the acute phase of Vogt-Koyanagi-Harada disease with serous retinal detachment were treated with pulse corticosteroid therapy. Optical coherence tomography was used to follow the resolution of the retinal detachment. RESULTS: Optical coherence tomography images showed a marked decrease in the retinal detachment immediately after the first intravenous injection of corticosteroid and subsequent resolution. CONCLUSION: The rapid improvement of serous retinal detachment associated with Vogt-Koyanagi-Harada disease following pulse corticosteroid therapy supports an early therapeutic mechanism related to improved permeability of capillaries and permeability of the blood-retinal barrier rather than an anti-inflammatory or immunosuppressive action.
