RESUMO
Culture filtrates of Alternaria brassicicola (Schw.) Wilts, were found to be toxic to secondary embryoids of Brassica napus ssp. oleifera (Metzg.) Sinsk. The culture filtrates were partially purified and added to tissue culture media to produce a selection medium. Different secondary embryoid lines of B. napus were found to differ in their sensitivity to the selection medium, and it was possible to select lines which showed increased resistance to the selection medium. Some plants that were regenerated from secondary embryoids were found to be more resistant to the pathogen than were seed-grown plants of the same cultivar, although there was no correlation between sensitivity to the selection medium and susceptibility to the pathogen.
RESUMO
Plants carrying Texas male-sterile (Tms) cytoplasm are normally sensitive to Drechslera maydis T-toxin. Tissue cultures were initiated from immature embryos of maize carrying Tms-cytoplasm, and plants were regenerated after selection for resistance to T-toxin. Fertile, T-toxin resistant plants were obtained from the unselected control cultures as well as from the selected material. In addition, one regenerant from an unselected culture was fertile and T-toxin sensitive. The progeny of the regenerants showed the phenotype of the female parent with respect to pollen-fertility, and T-toxin resistance. The data are consistent with the heritable changes observed being the result of the expression of an altered mitochondrial genome.
