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1.
Sci Rep ; 9(1): 11353, 2019 08 06.
Artigo em Inglês | MEDLINE | ID: mdl-31388019

RESUMO

Determining the Bovine Viral Diarrhoea (BVD) infection status of cattle herds is a challenge for control and eradication schemes. Given the changing dynamics of BVD  virus (BVDV) antibody responses in cattle, classifying herds based on longitudinal changes in the results of BVDV antibody tests could offer a novel, complementary approach to categorising herds that is less likely than the present system to result in a herd's status changing from year to year, as it is more likely to capture the true exposure dynamics of the farms. This paper describes the dynamics of BVDV antibody test values (measured as percentage positivity (PP)) obtained from 15,500 bovines between 2007 and 2010 from thirty nine cattle herds located in Scotland and Northern England. It explores approaches of classifying herds based on trend, magnitude and shape of their antibody PP trajectories and investigates the epidemiological similarities between farms within the same cluster. Gaussian mixture models were used for the magnitude and shape clustering. Epidemiologically meaningful clusters were obtained. Farm cluster membership depends on clustering approach used. Moderate concordance was found between the shape and magnitude clusters. These methods hold potential for application to enhance control efforts for BVD and other infectious livestock diseases.


Assuntos
Anticorpos Antivirais/sangue , Doença das Mucosas por Vírus da Diarreia Viral Bovina/imunologia , Vírus da Diarreia Viral Bovina/imunologia , Modelos Imunológicos , Animais , Bovinos , Inglaterra , Fazendas , Escócia , Fatores de Tempo
2.
Prev Vet Med ; 150: 143-150, 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-29153784

RESUMO

Antimicrobial resistance is primarily a problem in human medicine but there are unquantified links of transmission in both directions between animal and human populations. Quantitative assessment of the costs and benefits of reduced antimicrobial usage in livestock requires robust quantification of transmission of resistance between animals, the environment and the human population. This in turn requires appropriate measurement of resistance. To tackle this we selected two different methods for determining whether a sample is resistant - one based on screening a sample, the other on testing individual isolates. Our overall objective was to explore the differences arising from choice of measurement. A literature search demonstrated the widespread use of testing of individual isolates. The first aim of this study was to compare, quantitatively, sample level and isolate level screening. Cattle or sheep faecal samples (n=41) submitted for routine parasitology were tested for antimicrobial resistance in two ways: (1) "streak" direct culture onto plates containing the antimicrobial of interest; (2) determination of minimum inhibitory concentration (MIC) of 8-10 isolates per sample compared to published MIC thresholds. Two antibiotics (ampicillin and nalidixic acid) were tested. With ampicillin, direct culture resulted in more than double the number of resistant samples than the MIC method based on eight individual isolates. The second aim of this study was to demonstrate the utility of the observed relationship between these two measures of antimicrobial resistance to re-estimate the prevalence of antimicrobial resistance from a previous study, in which we had used "streak" cultures. Boot-strap methods were used to estimate the proportion of samples that would have tested resistant in the historic study, had we used the isolate-based MIC method instead. Our boot-strap results indicate that our estimates of prevalence of antimicrobial resistance would have been considerably lower in the historic study had the MIC method been used. Finally we conclude that there is no single way of defining a sample as resistant to an antimicrobial agent. The method used greatly affects the estimated prevalence of antimicrobial resistance in a sampled population of animals, thus potentially resulting in misleading results. Comparing methods on the same samples allows us to re-estimate the prevalence from other studies, had other methods for determining resistance been used. The results of this study highlight the importance of establishing what the most appropriate measure of antimicrobial resistance is, for the proposed purpose of the results.


Assuntos
Antibacterianos/farmacologia , Doenças dos Bovinos/epidemiologia , Farmacorresistência Bacteriana , Infecções por Escherichia coli/veterinária , Escherichia coli/efeitos dos fármacos , Doenças dos Ovinos/epidemiologia , Ampicilina/farmacologia , Animais , Bovinos , Doenças dos Bovinos/microbiologia , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Ácido Nalidíxico/farmacologia , Prevalência , Escócia/epidemiologia , Ovinos , Doenças dos Ovinos/microbiologia
3.
Epidemiol Infect ; 141(6): 1267-75, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22989449

RESUMO

Human campylobacteriosis exhibits a distinctive seasonality in temperate regions. This paper aims to identify the origins of this seasonality. Clinical isolates [typed by multi-locus sequence typing (MLST)] and epidemiological data were collected from Scotland. Young rural children were found to have an increased burden of disease in the late spring due to strains of non-chicken origin (e.g. ruminant and wild bird strains from environmental sources). In contrast the adult population had an extended summer peak associated with chicken strains. Travel abroad and UK mainland travel were associated with up to 17% and 18% of cases, respectively. International strains were associated with chicken, had a higher diversity than indigenous strains and a different spectrum of MLST types representative of these countries. Integrating empirical epidemiology and molecular subtyping can successfully elucidate the seasonal components of human campylobacteriosis. The findings will enable public health officials to focus strategies to reduce the disease burden.


Assuntos
Infecções por Campylobacter/etiologia , Adolescente , Adulto , Fatores Etários , Idoso , Animais , Animais Selvagens/microbiologia , Aves/microbiologia , Infecções por Campylobacter/epidemiologia , Galinhas/microbiologia , Criança , Pré-Escolar , Humanos , Incidência , Pessoa de Meia-Idade , Epidemiologia Molecular/métodos , Tipagem de Sequências Multilocus , População Rural/estatística & dados numéricos , Escócia/epidemiologia , Estações do Ano , Viagem , População Urbana/estatística & dados numéricos , Adulto Jovem
4.
Vet Parasitol ; 188(1-2): 120-6, 2012 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-22469484

RESUMO

The faecal egg count (FEC) is the most widely used means of quantifying the nematode burden of horses, and is frequently used in clinical practice to inform treatment and prevention. The statistical process underlying the FEC is complex, comprising a Poisson counting error process for each sample, compounded with an underlying continuous distribution of means between samples. Being able to quantify the sources of variability contributing to this distribution of means is a necessary step towards providing estimates of statistical power for future FEC and FECRT studies, and may help to improve the usefulness of the FEC technique by identifying and minimising unwanted sources of variability. Obtaining such estimates require a hierarchical statistical model coupled with repeated FEC observations from a single animal over a short period of time. Here, we use this approach to provide the first comparative estimate of multiple sources of within-horse FEC variability. The results demonstrate that a substantial proportion of the observed variation in FEC between horses occurs as a result of variation in FEC within an animal, with the major sources being aggregation of eggs within faeces and variation in egg concentration between faecal piles. The McMaster procedure itself is associated with a comparatively small coefficient of variation, and is therefore highly repeatable when a sufficiently large number of eggs are observed to reduce the error associated with the counting process. We conclude that the variation between samples taken from the same animal is substantial, but can be reduced through the use of larger homogenised faecal samples. Estimates are provided for the coefficient of variation (cv) associated with each within animal source of variability in observed FEC, allowing the usefulness of individual FEC to be quantified, and providing a basis for future FEC and FECRT studies.


Assuntos
Fezes/parasitologia , Helmintíase Animal/diagnóstico , Doenças dos Cavalos/parasitologia , Contagem de Ovos de Parasitas/veterinária , Animais , Doenças dos Cavalos/diagnóstico , Cavalos , Contagem de Ovos de Parasitas/métodos , Estações do Ano
5.
Prev Vet Med ; 99(2-4): 211-24, 2011 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-21277032

RESUMO

Meticillin-resistant Staphylococcus aureus (MRSA) is an important nosocomial and community-acquired pathogen with zoonotic potential. The relationship between MRSA in humans and companion animals is poorly understood. This study presents a quantitative exposure assessment, based on expert opinion and published data, in the form of a second order stochastic simulation model with accompanying logistic regression sensitivity analysis that aims to define the most important factors for MRSA acquisition in dogs. The simulation model was parameterised using expert opinion estimates, along with published and unpublished data. The outcome of the model was biologically plausible and found to be dominated by uncertainty over variability. The sensitivity analysis, in the form of four separate logistic regression models, found that both veterinary and non-veterinary routes of acquisition of MRSA are likely to be relevant for dogs. The effects of exposure to, and probability of, transmission of MRSA from the home environment were ranked as the most influential predictors in all sensitivity analyses, although it is unlikely that this environmental source of MRSA is independent of alternative sources of MRSA (human and/or animal). Exposure to and transmission from MRSA positive family members were also found to be influential for acquisition of MRSA in pet dogs, along with veterinary clinic attendance and, while exposure to and transmission from the veterinary clinic environment was also found to be influential, it was difficult to differentiate between the importance of independent sources of MRSA within the veterinary clinic. The implementation of logistic regression analyses directly to the input/output relationship within the simulation model presented in this paper represents the application of a variance based sensitivity analysis technique in the area of veterinary medicine and is a useful means of ranking the relative importance of input variables.


Assuntos
Doenças do Cão/microbiologia , Staphylococcus aureus Resistente à Meticilina/patogenicidade , Infecções Estafilocócicas/veterinária , Animais , Simulação por Computador , Doenças do Cão/transmissão , Cães , Microbiologia Ambiental , Feminino , Modelos Logísticos , Masculino , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Medição de Risco , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/transmissão , Processos Estocásticos , Zoonoses
6.
Epidemiol Infect ; 138(12): 1744-7, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20587120

RESUMO

During a 15-month period in Scotland a small but important number of human Campylobacter cases (3·2%) arose from 91 putative household outbreaks. Of the 26 outbreaks with known strain composition, 89% were composed of the same MLST which supports the potential use of MLST in public health epidemiology. The number of cases associated with household outbreaks is much larger than general outbreaks and there is some evidence to indicate that there may be secondary transmission, although this is relatively rare.


Assuntos
Técnicas de Tipagem Bacteriana , Infecções por Campylobacter/epidemiologia , Campylobacter/classificação , Campylobacter/isolamento & purificação , Impressões Digitais de DNA , Surtos de Doenças , Saúde da Família , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Campylobacter/genética , Criança , Pré-Escolar , Análise por Conglomerados , Características da Família , Feminino , Genótipo , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Escócia/epidemiologia , Análise de Sequência de DNA , Adulto Jovem
7.
Vet Parasitol ; 169(3-4): 335-9, 2010 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-20138432

RESUMO

A cross-sectional coprological survey in the tropical regions of Ada, Akaki, Bereh and Boset, and a retrospective post-mortem investigation were conducted to study the epidemiology of fasciolosis in working donkeys in Ethiopia. Faecal samples from 803 donkeys were collected, and the number of liver flukes recovered from 112 donkeys at post-mortem between 1995 and 2004 were analysed. There was a high prevalence of fasciolosis irrespective of the age of the donkeys. The overall prevalence of the infection was 44.4% in coprologically examined donkeys, and the prevalence in the donkeys examined post-mortem was 41.9%. The infection prevalence was significantly higher in Bereh and Ada regions than in Akaki and Boset regions. Bereh with 72.6% and Boset with 21.5% showed a significantly higher and lower infection prevalence, respectively, than the rest of the regions (P<0.001). There was no significant difference between different age groups of donkeys in the infection prevalence (P>0.05) but infection intensity was significantly higher in donkeys 8 years old and above (P<0.0001). Both Fasciola hepatica and Fasciola gigantica were identified.


Assuntos
Equidae/parasitologia , Fasciolíase/veterinária , Animais , Etiópia/epidemiologia , Fasciola hepatica/isolamento & purificação , Fasciolíase/epidemiologia , Fezes/parasitologia , Feminino , Masculino , Prevalência
8.
Prev Vet Med ; 93(4): 316-23, 2010 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-19962203

RESUMO

The Faecal Egg Count Reduction Test (FECRT) is the most widely used method of assessing the efficacy of anthelmintics, and is the only in vivo technique currently approved for use with horses. Equine Faecal Egg Count (FEC) data are frequently characterised by a low mean, high variability, small sample size and frequent zero count observations. Accurate analysis of the data therefore depends on the use of an appropriate statistical technique. Analyses of simulated FECRT data by methods based on calculation of the empirical mean and variance, non-parametric bootstrapping, and Markov chain Monte Carlo (MCMC) are compared. The MCMC method consistently outperformed the other methods, independently of the distribution from which the data were generated. Bootstrapping produced notional 95% confidence intervals containing the true parameter as little as 40% of the time with sample sizes of less than 50. Analysis of equine FECRT data yielded inconclusive results in 53 of 63 (84%) datasets, suggesting that the routine use of prior sample size calculations should be adopted to ensure sufficient data are collected. The authors conclude that computationally intensive parametric methods such as MCMC be used for analysis of FECRT data with sample sizes of less than 50, in order to avoid erroneous inference about the true efficacy of anthelmintics in the field.


Assuntos
Anti-Helmínticos/uso terapêutico , Fezes/parasitologia , Helmintíase Animal/tratamento farmacológico , Doenças dos Cavalos/tratamento farmacológico , Contagem de Ovos de Parasitas/veterinária , Animais , Helmintíase Animal/epidemiologia , Cavalos , Países Baixos/epidemiologia , Contagem de Ovos de Parasitas/métodos
9.
Zoonoses Public Health ; 57(7-8): 487-92, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19638164

RESUMO

The spatial and temporal epidemiology of human cryptosporidiosis was described by analysing sporadic cases reported in Scotland from 2005 to 2007. Measures of livestock density and human population density were explored as indicators of the geographical variation in prevalence. Cryptosporidium parvum was more common in areas with lower human population densities, with a higher ratio of the number of farms to human inhabitants and with a higher ratio of the number of private water supplies to human inhabitants. Cryptosporidium parvum caused disease in humans in rural areas and in areas with high ruminant livestock density, whereas Cryptosporidium hominis was more common in the more densely human populated areas of Scotland. The association of private water supplies and increased Cryptosporidium reports merits further public health efforts.


Assuntos
Criptosporidiose/epidemiologia , Cryptosporidium/isolamento & purificação , Surtos de Doenças/estatística & dados numéricos , Abastecimento de Água , Adolescente , Adulto , Distribuição por Idade , Idoso , Idoso de 80 Anos ou mais , Criação de Animais Domésticos , Animais , Criança , Pré-Escolar , Criptosporidiose/parasitologia , Cryptosporidium/classificação , Feminino , Humanos , Incidência , Lactente , Recém-Nascido , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Vigilância da População , Saúde Pública , População Rural , Escócia/epidemiologia , Estações do Ano , População Urbana , Adulto Jovem
10.
Equine Vet J ; 41(5): 428-32, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19642401

RESUMO

REASONS FOR PERFORMING STUDY: Time delays between collection of blood samples and biochemical analysis of equine blood are unavoidably common in equine practice. The effect that delays may have on the accuracy of results of blood biochemical analyses is not well established. HYPOTHESIS: Delays in processing of blood of up to 72 h results in alterations in measured levels of common biochemical analytes that are of potential clinical relevance. Separation of serum prior to storage is protective against the effects of time delays. METHODS: Samples of clotted blood, separated serum and oxalate fluoride plasma from 20 horses were stored and analysed at 0, 24, 48 and 72 h. Graphical exploration of each analyte was undertaken. General linear models with fixed effects were fitted for the whole blood data. The mean bias and 95% limits of agreement were calculated, using bootstrapped data, to assess agreement between pairs of samples analysed at 0 h and other time points. Bland-Altman plots were used to explore general trends in the data. Paired t tests were used to compare the results from whole blood and separated serum. RESULTS: Delays in processing equine blood resulted in significant increases in measured concentrations of aspartate aminotransferase, creatine kinase, lactate dehydrogenase, total bile acids and magnesium. A significant decrease in concentration was identified for glucose (serum and oxalate fluoride preserved plasma). Separation of serum immediately following clot formation resulted in nonsignificant increases in accuracy for some analytes. CONCLUSIONS AND PRACTICAL SIGNIFICANCE: Delays in processing of blood samples may result in biochemical changes of clinical relevance in individual cases; however, in the majority of cases, where delays are only a few days and a number of analytes are assessed concurrently, delays are unlikely to have an effect on the interpretation of results. Separation of serum following clot formation is of limited benefit. Clinical samples in which a delay in processing has occurred may be interpreted with reference to the data presented.


Assuntos
Análise Química do Sangue/veterinária , Cavalos/sangue , Manejo de Espécimes/veterinária , Temperatura , Animais , Preservação de Sangue
11.
Parasitology ; 135(10): 1225-35, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18620622

RESUMO

Understanding the frequency distribution of parasites and parasite stages among hosts is essential for efficient experimental design and statistical analysis, and is also required for the development of sustainable methods of controlling infection. Nematodirus battus is one of the most important organisms that infect sheep but the distribution of parasites among hosts is unknown. An initial analysis indicated a high frequency of animals without N. battus and with zero egg counts, suggesting the possibility of a zero-inflated distribution. We developed a Bayesian analysis using Markov chain Monte Carlo methods to estimate the parameters of the zero-inflated negative binomial distribution. The analysis of 3000 simulated data sets indicated that this method out-performed the maximum likelihood procedure. Application of this technique to faecal egg counts from lambs in a commercial upland flock indicated that N. battus counts were indeed zero-inflated. Estimating the extent of zero-inflation is important for effective statistical analysis and for the accurate identification of genetically resistant animals.


Assuntos
Infecções por Nematoides/veterinária , Doenças dos Ovinos/parasitologia , Animais , Teorema de Bayes , Feminino , Masculino , Cadeias de Markov , Método de Monte Carlo , Infecções por Nematoides/epidemiologia , Contagem de Ovos de Parasitas/veterinária , Escócia , Ovinos/parasitologia , Doenças dos Ovinos/epidemiologia
12.
Vet Rec ; 162(6): 177-80, 2008 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-18263917

RESUMO

A cross-sectional coprological survey in the regions of Ada, Akaki, Bereh and Boset, and a retrospective postmortem investigation were conducted to study the epidemiology of Parascaris equorum in donkeys and horses in Ethiopia. Faecal samples from 803 working donkeys and 402 horses were collected, and the numbers of worms recovered from 112 donkeys examined postmortem between 1995 and 2004 were analysed. There was a high prevalence of infection and faecal egg output of P equorum in both donkeys and horses, and the severity of the infection in donkeys was increased irrespective of their age. The prevalence of the infection in the donkeys was 51.1 per cent and in the horses 16.2 per cent, and the prevalence in the donkeys examined postmortem was 55 per cent. There was no significant difference between different age groups of donkeys in either the prevalence or the intensity of the infection. The prevalence of the infection was significantly higher in the Ada and Akaki regions than in the Bereh and Boset regions.


Assuntos
Infecções por Ascaridida/veterinária , Ascaridoidea/isolamento & purificação , Equidae/parasitologia , Fezes/parasitologia , Doenças dos Cavalos/epidemiologia , Fatores Etários , Animais , Infecções por Ascaridida/epidemiologia , Cadáver , Estudos Transversais , Etiópia/epidemiologia , Feminino , Cavalos , Masculino , Contagem de Ovos de Parasitas/veterinária , Prevalência , Estudos Retrospectivos
13.
Vet Immunol Immunopathol ; 120(1-2): 3-9, 2007 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-17765323

RESUMO

Detecting some of the genes that influence disease resistance would improve our understanding of the processes that cause disease and also simplify disease control. Genes within the major histocompatibility complex (mhc) are strong candidates for disease resistance and they have been intensely studied for the last 30 years. Recently, several groups working independently have reported the existence of alleles within the mhc that are associated with enhanced resistance to nematode infection. This article uses hindsight to describe some of the potential pitfalls that hinder the search for valid disease resistance genes. The search requires a good understanding of disease biology, molecular genetics, statistical genetics and especially, the design and analysis of experiments. The power to detect mhc effects is quite low and is quite sensitive to the frequency of the putative resistance alleles.


Assuntos
Predisposição Genética para Doença , Complexo Principal de Histocompatibilidade/genética , Infecções por Nematoides/veterinária , Doenças dos Ovinos/genética , Alelos , Animais , Perfilação da Expressão Gênica , Infecções por Nematoides/genética , Infecções por Nematoides/imunologia , Ovinos , Doenças dos Ovinos/imunologia , Doenças dos Ovinos/parasitologia
14.
Prev Vet Med ; 80(4): 257-70, 2007 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-17485125

RESUMO

In the slaughter processing of cattle, contaminated hides have been identified as one of the major sources of Escherichia coli O157 carcase contamination. Logistic regression analysis was applied to data collected in a large scale study in Scotland involving 222 cattle forming 34 groups sent for slaughter from 30 farms to 10 slaughterhouses. Aspects of individual animal characteristics, farm management practices and slaughterhouse features were examined to identify potential risk factors for hide contamination at harvest. Two models were developed, the first in which slaughterhouse was modelled as a fixed effect, and a second model where slaughterhouse and farm groups were modelled as random effects. In the first model, there was a significantly increased risk of a carcase testing positive for E. coli O157 on the hide if either the hide of the carcase immediately before or after it on the line was contaminated (OR 3.6; 95% CI: 1.4-9.9). If both adjacent carcases had contaminated hides, the odds ratio for the study carcase having a contaminated hide rose to 11.5 (95% CI: 4.4-32.5). If animals were held in lairage, receiving hay as feed appeared to have a protective effect on hide contamination. Transportation to the slaughterhouse by haulier, as opposed to transport by the farmer, was associated with a 5.4 increase in the odds of E. coli O157 contamination. The use of a crush in the lairage, often employed when reading ear tags, was also found to significantly increase the odds of hide contamination with E. coli O157. In the second model, the inclusion of slaughterhouse and farm group as random effects resulted in two of the previously identified factors being associated with hide contamination. If at least one of the adjacent carcases on the line had a contaminated hide, the associated odds ratio was 6.6 (95% CI: 2.8-15.9), which rose to 22.7 (95% CI: 9.3-55.5) if both adjacent hides were contaminated. Receiving hay in lairage was found to be important to the model, although not significant in itself (OR 0.005; 95% CI: 1.2e(-6)-20.7). These results suggest that modifiable risk factors for hide contamination exist. However, in order best to reduce the prevalence of hide contamination at slaughter, individual slaughterhouse risk assessment and intervention strategies are appropriate.


Assuntos
Matadouros , Bovinos/microbiologia , Escherichia coli O157/isolamento & purificação , Contaminação de Alimentos , Manipulação de Alimentos/métodos , Pele/microbiologia , Criação de Animais Domésticos , Animais , Modelos Logísticos , Modelos Biológicos , Razão de Chances , Fatores de Risco , Escócia
16.
J Vet Intern Med ; 20(2): 388-94, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16594599

RESUMO

A urine sucrose test has recently been reported to be a reliable method of detecting gastric ulcers in horses; however, technical difficulties associated with urine collection have limited the practical value of the test. The objective of this pilot study was to determine whether gastric sucrose permeability, as evaluated by serum sucrose concentration, could be used to detect gastric mucosal injury in horses. Twelve adult horses with naturally acquired gastric ulceration were studied. After a 20-hour nonfeeding period, each horse was dosed with 250 g of sucrose via nasogastric intubation. Blood samples were collected at 0, 15, 30, 45, 60, and 90 minutes, and horses underwent gastroscopy 4 hours later. The severity of gastric ulceration in each horse was defined by means of a 4-point ulcer-scoring system, and the relationship with serum sucrose concentration was analyzed by means of a linear mixed-effects model. Serum sucrose concentration was measured by liquid chromatography operating in tandem with electrospray mass spectrometry. After nasogastric administration of table sugar, horses with moderate to severe gastric ulceration had significant increase in serum sucrose concentration at 30, 45, 60, and 90 minutes, relative to earlier times (P < .05). Peak sucrose concentration was observed at 45 minutes, and was correlated with ulcer severity (Spearman's rank correlation coefficient = 0.898, P < .05). These data indicate that determination of sucrose concentration in equine serum may be a useful test for identifying horses with endoscopically visible gastric ulceration and has potential use as a noninvasive method for screening and monitoring horses engaged in racing training and other performance-related disciplines.


Assuntos
Mucosa Gástrica/fisiopatologia , Doenças dos Cavalos/sangue , Doenças dos Cavalos/fisiopatologia , Cavalos/sangue , Úlcera Gástrica/veterinária , Sacarose/sangue , Animais , Feminino , Doenças dos Cavalos/diagnóstico , Masculino , Permeabilidade , Úlcera Gástrica/diagnóstico , Úlcera Gástrica/fisiopatologia , Sacarose/administração & dosagem
17.
Epidemiol Infect ; 133(6): 1033-41, 2005 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16274499

RESUMO

In Scotland, between 1995 and 2000 there were between 4 and 10 cases of illness per 100000 population per year identified as being caused by Escherichia coli O157, whereas in England and Wales there were between 1 and 2 cases per 100000 population per year. Within Scotland there is significant regional variation. A cluster of high rate areas was identified in the Northeast of Scotland and a cluster of low rate areas in central-west Scotland. Temporal trends follow a seasonal pattern whilst spatial effects appeared to be distant rather than local. The best-fit model identified a significant spatial trend with case rate increasing from West to East, and from South to North. No statistically significant spatial interaction term was found. In the models fitted, the cattle population density, the human population density, and the number of cattle per person were variously significant. The findings suggest that rural/urban exposures are important in sporadic infections.


Assuntos
Surtos de Doenças/estatística & dados numéricos , Infecções por Escherichia coli/epidemiologia , Escherichia coli O157/isolamento & purificação , Animais , Bovinos , Humanos , Modelos Logísticos , Escócia/epidemiologia
18.
Vet Immunol Immunopathol ; 108(1-2): 53-7, 2005 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-16099055

RESUMO

Lambs with the G2 allele at the ovine major histocompatibility complex (mhc) class II locus DRB1 has previously been shown to have lower faecal nematode egg counts than lambs with the I allele at this locus. This association has been confirmed in separate cohorts from the same farm. Other alleles within the mhc have also shown associations with nematode resistance in other breeds of sheep. Therefore, variation in the mhc is responsible for part of the observed genetic variation in resistance to nematode infection. In addition to the specific effect of particular alleles, heterozygotes are also more resistant than homozygotes. This heterozygote advantage is capable of maintaining the high levels of polymorphism observed within the mhc.


Assuntos
Complexo Principal de Histocompatibilidade , Polimorfismo Genético , Ovinos/genética , Ovinos/imunologia , Alelos , Animais , Sequência de Bases , Evolução Biológica , DNA/genética , Heterozigoto , Homozigoto , Interações Hospedeiro-Parasita/genética , Interações Hospedeiro-Parasita/imunologia , Infecções por Nematoides/genética , Infecções por Nematoides/imunologia , Infecções por Nematoides/veterinária , Contagem de Ovos de Parasitas , Ovinos/parasitologia , Doenças dos Ovinos/genética , Doenças dos Ovinos/imunologia , Doenças dos Ovinos/parasitologia
19.
Vet Rec ; 157(9): 245-50, 2005 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-16127133

RESUMO

A survey was conducted of the prevalence of environmental pathogens, especially Streptococcus uberis, as causes of clinical mastitis in dairy cows. The response of intramammary infections with S uberis to conventional treatment was monitored by taking milk samples for bacteriology and somatic cell counting seven, 14 and 21 days after the treatment. The results showed that 51 per cent of the infections failed to respond, and the odds of cases failing to respond was significantly increased when the individual quarter somatic cell count seven days after the treatment was greater than 201,000 cells/ml. Ninety-six per cent of the suspected S uberis isolates identified by culture were confirmed as S uberis by using the api 20 Strep system. Restriction endonuclease fingerprinting was used to type the strains of S uberis isolated from 75 milk samples from 32 cows. Analysis showed that 96 per cent of the cases of S uberis that failed to respond to conventional treatment were persistent infections with one strain rather than reinfections with different strains. The persistent cases of S uberis were treated further with an extended course of intramammary preparations containing either procaine penicillin with dihydrostreptomycin or cefquinome. There was no significant difference between the cure rates achieved by the two preparations, and 55 per cent of the cases that had failed to respond to conventional treatment responded to the additional treatment.


Assuntos
Antibacterianos/uso terapêutico , Mastite Bovina/tratamento farmacológico , Leite/microbiologia , Streptococcus/isolamento & purificação , Animais , Bovinos , Feminino , Mastite Bovina/diagnóstico , Streptococcus/classificação , Streptococcus/patogenicidade , Resultado do Tratamento
20.
Parasitology ; 129(Pt 3): 363-9, 2004 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-15471011

RESUMO

The number of Teladorsagia circumcincta 4th-stage larvae in naturally infected lambs from a single farm varied among lambs and among different years. Within each year the distribution of 4th-stage larvae among lambs was similar to that expected from a negative binomial distribution. The ratio of 4th-stage larvae to adult T. circumcincta was low in two years with a low mean intensity of infection but high in two years with a higher mean intensity of infection. The negative binomial distribution is defined by the mean and by k, a parameter that measures dispersion; k was low when mean infection intensity was low but higher when mean infection intensity was high. As k is an inverse index of overdispersion this indicated that the distribution of 4th-stage larvae was more overdispersed at low levels of infection. In a combined analysis, the number of adult T. circumcincta and the plasma IgA activity against 4th-stage larvae were both associated with increased numbers of 4th-stage larvae. There was a statistical interaction between the number of adults and IgA activity that moderated their combined effect.


Assuntos
Imunoglobulina A/imunologia , Doenças dos Ovinos/imunologia , Doenças dos Ovinos/parasitologia , Trichostrongyloidea/crescimento & desenvolvimento , Tricostrongiloidíase/veterinária , Abomaso/parasitologia , Animais , Distribuição Binomial , Feminino , Imunoglobulina A/sangue , Intestino Delgado/parasitologia , Larva/crescimento & desenvolvimento , Modelos Lineares , Masculino , Análise de Regressão , Ovinos , Trichostrongyloidea/imunologia , Tricostrongiloidíase/imunologia , Tricostrongiloidíase/parasitologia
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