1.
Biosci Biotechnol Biochem
; 88(1): 74-78, 2023 Dec 19.
Artigo
em Inglês
| MEDLINE
| ID: mdl-37766390
RESUMO
Recombinant 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase (ErtC) derived from Burkholderia sp. HME13 was purified to homogeneity. Here, ErtC's kinetic parameters, optimum reaction temperature and pH, and stability at varying temperatures and pH and the effects of various additives on ErtC activity were determined. Real-time polymerase chain reaction and enzyme assays suggested that ergothioneine induced the expression of ertC.