RESUMO
Various anti-inflammatory compounds were tested for their influence on the life span of the fruit fly (Drosophila melanogaster). 2,3-dihydroxybenzoic acid increased the median life span by 10.9% when fed to adults at a concentration of 1 X 10(-5)M for the entire adult life span. All other anti-inflammatory compounds including acetylsalicylic acid, acetylsalicylic acid plus cupric chloride, Bufferin, chlorpromazine HC1, dimethylsulfoxide, indomethacin, phenol, salicylic acid, and sodium salicylate either decreased or did not change the life span. Rearing flies on 0.5% (0.060M) dimethylsulfoxide caused a 33.3% reduction in life span compared with an 11.8% reduction when fed during adulthood only. At low doses all of the anti-inflammatory compounds including dimethylsulfoxide proved to be nontoxic as measured by changes in life span.
Assuntos
Envelhecimento/efeitos dos fármacos , Anti-Inflamatórios/farmacologia , Animais , Clorpromazina/farmacologia , Cobre/farmacologia , Dimetil Sulfóxido/farmacologia , Drosophila melanogaster , Hidroxibenzoatos/farmacologia , Indometacina/farmacologia , Masculino , Fenol , Fenóis/farmacologia , Salicilatos/farmacologiaRESUMO
Superoxide dismutase activity in brains of male C57BL/6J mice decreased with age by 36% on a protein base and by 32% on a DNA base between 50 and 900 days of age. Brain copper increased by 45% up to 600 days of age and appeared to increase slightly from 600 to 900 days of age. Feeding of copper gluconate (5 x 10(-3)M) failed to change the level of copper in either young or old brain, and superoxide dismutase activity was not significantly increased or inhibited by copper feeding. Cupric chloride (2.35 x 10(-6)M) was found to inhibit purified superoxide dismutase (2 x 10(-8)M) by 50%. These observations indicate that brain copper concentrations do not directly determine the activity levels of the copper containing enzyme, superoxide dismutase, under normal ageing conditions.
