[Complex evaluation of the feto-placental system in gestosis of different degree of severity]. / Kompleksnaia otsenka feto-platsentarnoi sistemy pri gestozakh razlichnoi stepeni tiazhesti.

Comprehensive studies of the fetoplacental system in pregnant women with gestoses of varying severity (n = 260) and in those with normal pregnancy (n = 200), carried out in pregnancy terms of 29 to 41 weeks, have revealed a lowering of the levels of trophoblastic beta-globulin and pregnancy-associated alpha 2-glycoprotein, elevated concentration of pregnancy-associated protein A, a higher incidence of placental types II and III (as evidenced by ultrasonic examination), reduced rate of growth of the fetal biometric parameters and a reduction of fetal systolic output, augmenting with the gestosis severity. A comprehensive assessment of the fetoplacental system permits obtain a more complete picture of the gestosis severity.

[An immunochemical study of the interaction of human trophoblastic beta-glycoprotein with steroid and sex hormones]. / Immunokhimicheskoe izuchenie vzaimodeistviia trofoblasticheskogo beta-glikoproteina cheloveka s steroidnymi i polovymi gormonami.

Human trophoblastic beta-glycoprotein (TBG) was subjected to immunoelectrophoresis with intermediate gel containing hormones. We have observed TBG binding either with cyclopentaneperhydrophenanthrene derivatives (cholesterol, testosterone, progesterone, estradiol, estrone) or their synthetic analogs (sinestrol, diethylstilbestrol). TGB showed markedly higher affinity to estrogens than to other hormones.

[Immunochemical analysis of lectin receptors in the structure of trophoblastic beta 1-glycoprotein and pregnancy-associated alpha 2-glycoprotein]. / Immunokhimicheskii analiz "retseptorov" k lektinam v strukture trofoblasticheskogo beta 1-glikoproteina i assotsiirovannogo s beremennost'i al'fa 2-glikoproteina.

Trophoblastic beta-glycoprotein (TBG), pregnancy-associated alpha 2-glycoprotein (AGP) and alpha-fetoprotein (AFP) bind to concanavalin A, phytohemagglutinin P and pea lectin. Lentil lectin interacts with TBG only, whereas peanut lectin and castor bean lectin are characterized by affinity to AGP. Hence, TBG, AGP and AFP contain the following carbohydrate components: alpha-D-mannose, alpha-D-glucose, N-acetyl-D-glucosamine and N-acetyl-D-galactosamine. In addition, AGP contains beta-D-galactose.

[Immunochemical study of the heterogeneity of trophoblastic beta 1-glycoprotein]. / Immunokhimicheskoe izuchenie geterogennosti trofoblasticheskogo beta 1-glikoproteina.

It has been found that in mild acid (pH 6.6) and mild-alkaline media (pH 7.7) both pregnancy proteins form complete precipitates. In more alkaline buffer solutions the form of alpha 2-glycoprotein (alpha 2-GP) precipitate is preserved, while trophoblastic beta 1-glycoprotein (TBG) shows three immunochemically identical components with different electrophoretic mobility. The form with beta-globulins mobility predominates, and minor fragments are presented by alpha- and gamma-components. All TBG forms are clearly seen at pH 8.6. In more alkaline medium (pH 10.0) the clarity of the precipitates drastically decreases. It is shown that heparin introduction into the gel of first dimension electrophoresis increases anode electrophoretic mobility of both proteins at polysaccharide concentration of at least 0.1 mg/ml. Large amounts of heparin cause the increase in TBG alpha-component precipitate area and the decrease in the form with beta-globulins mobility. At the same time alpha 2-GP precipitate area and form remain unchanged.

[Competitive inhibition by carbohydrates of reactions between phytohemagglutinin II and plasma glycoproteins]. / Konkurentnoe ingibirovanie uglevodami reaktsii mezhdu fitogemaggliutininom II i glikoproteinami plazmy krovi.

Competitive reactions between free carbohydrates and the carbohydrates of blood plasma glycoproteins for receptors of phytohemagglutinin II were studied by means of modified cross affinity immunoelectrophoresis. These reactions exhibited both specific and unspecific properties. Possible mechanisms of these reactions are discussed as well as their possible use in studies of properties of carbohydrate-containing biopolymers.

[Interaction of pregnancy proteins with phytohemagglutinin P and concanavalin A (con A)]. / Vzaimodeistvie belkov beremennosti s fitohemaggliutininom P i konkanavalinom A (kon A).

It has been established that trophoblast-specific beta-glycoprotein and pregnancy-associated alpha 2-glycoprotein specifically react with non-fixed PHA and Con A. Affinity to the former protein is significantly higher than to the latter one. alpha-Fetoprotein has a low affinity to Con A alone. Affinity to this lectin is in an agreement with the content of carbohydrates contained by pregnancy proteins. A considerable part of human serum proteins bind with Con A; receptors for PHA possess only some serum proteins. As the above-mentioned lectins are often used for stimulation of lymphocyte blast transformation, in is recommended that the constituent parts of the culture medium should be preliminarily tested to specify more accurately their affinity to PHA and Con A.