Recent advances in gold nanoparticles-based biosensors for tuberculosis determination.

Tuberculosis (TB) is one of the major killer diseases affecting lung parenchymal tissues. Mycobacterium tuberculosis (Mtb) is the bacterium that causes it. It most commonly affects the lungs, although it can affect any part of the body, including the stomach, glands, bones, and nervous system. Although anti-mycobacterial drugs are available, it remains a major threat to public health due to the rise of drug-resistant strains, and early and accurate diagnosis is very important. Currently, research science and medical communities are focusing on the use of cost-effective biosensors to manage human biological processes and assess accurate health diagnostics. Due to their high sensitivity in chemical and biological assays, nanomaterials have been considered in the field of biosensors for better diagnosis, and among them, gold nanoparticles (AuNPs) can play an important role in accelerating the diagnosis of TB. Superior biocompatibility, conductivity, catalytic properties, high surface-to-volume ratio, and high density enable their widespread use in the fabrication of biosensors. This review evaluates the diagnostic accuracy of AuNP-based biosensors for the detection of Mtb. According to different transducers of biosensors, their structure, performance, advantages and limitations are summarized and compared. Moreover, the upcoming challenges in their analytical performance have been highlighted and the strategies to overcome those challenges have been briefly discussed.

In vitro and in vivo studies of Dragon's blood plant (D. cinnabari)-loaded electrospun chitosan/PCL nanofibers: Cytotoxicity, antibacterial, and wound healing activities.

The D. cinnabari plant was loaded into the chitosan (Chn)/polycaprolactone (PCL) nanofibers in two forms: resin (D. cinnabari) and its ethyl acetate fraction. The Chn/PCL, Chn/PCL/D. cinnabari (CPD, 1, 3, and 5 %), and Chn/PCL/ethyl acetate extract D. cinnabari (CPED, 1, 3, and 5 %) showed no toxicity against human dermal fibroblast cells. The lactate dehydrogenase assay results indicated that the toxicity of pour, coated D. cinnabari, and CPED nanofibers were lower than 10 and 15 % after 1 and 3 days, respectively. The antibacterial results showed the inhibition zone for ethyl acetate extract D. cinnabari (ED-3 %), the Chn/PCL-2, and CPED3% nanofibers was 8.1, 7.4, 4.2, 5.1 mm, 12.8, 12.4, 21.7, 17.2 mm, and 24.7, 22.9, 37.1, 30.2 mm against S. aureus, B. subtilis, E. coli, and P. aeruginosa, respectively. The antibacterial activity results showed synergistic effect between the Chn/PCL and ethyl acetate extract D. cinnabari occurred. The diameter of wounds (1.50 × 1.50 cm diameter) made on the dorsal surface of rabbits reduced to 1.50 × 0.70, 0.50 × 0.30, 1.00 × 1.00, 0.60 × 0.50, 0.20 × 0.05, and 0.00 × 0.00 cm in the presence of ordinary gauze dressing, silver sulfadiazine, ED-3 %, Chn/PCL-2, CPD3%, and CPED3%nanofibers, respectively, after 14 days.

Incorporation of doxorubicin and CoFe2O4 nanoparticles into the cellulose acetate phthalate / polyvinyl alcohol (core)/ polyurethane (shell) nanofibers against A549 human lung cancer during chemotherapy/hyperthermia combined method.

Cellulose acetate phthalate (CAP)/polyvinyl alcohol (PVA)/polyurethane (PU) nanofibers were synthesized by simple and coaxial electrospinning (ES) processes. Doxorubicin (DOX) and the CoFe2O4 nanoparticles were loaded into the nanofibers. The performance of the prepared nanofibers was investigated for the sustained release of DOX against A541 lung cancer cells under chemotherapy/external magnetic field (EMF) and alternating magnetic field (AMF, hyperthermia treatment) combined methods in both the in vitro and in vivo conditions. The sustained release of DOX from core-shell nanofibers containing 5 wt% cobalt ferrite was obtained within 300, 600 h, at pH of 5.5 and 7.4 without AMF and 168, 360 h, under an alternating magnetic field (AMF). More than 98.3 ± 0.2 % of A549 cancer cells were killed in the presence of core-shell nanofibers containing 100 µg DOX and 5 % cobalt ferrite nanoparticles in the presence of AMF. The flowcytometric results indicated that only 19.1 and 8.85 % cancer cells remained alive under EMF and AMF, respectively. The in vivo results revealed in stopping the growth of tumor volume and decrease in the relative tumor volume up to 0.5 were obtained using magnetic core-shell nanofibers containing 100 µg DOX and 5 % cobalt ferrite nanoparticles in the presence of EMF and AMF, respectively.

Additive Manufacturing of an Extended-Release Tablet of Tacrolimus.

An extended-release tablet of tacrolimus as once-daily dosing was fabricated using 3D printing technology. It was developed by combining two 3D-printing methods in parallel. Indeed, an optimized mixture of PVA, sorbitol, and magnesium stearate as a shell compartment was printed through a hot-melt extrusion (HME) nozzle while an HPMC gel mixture of the drug in the core compartment was printed by a pressure-assisted micro-syringe (PAM). A 3D-printed tablet with an infill of 90% was selected as an optimized formula upon the desired dissolution profile, releasing 86% of the drug at 12 h, similar to the commercial one. The weight variation, friability, hardness, assay, and content uniformity determination met USP requirements. A microbial evaluation showed that the 3D-printed tablet does not support microbial growth. SEM analysis showed smooth surfaces with multiple deposited layers. No peak interference appeared based on FTIR analysis. No decomposition of the polymer and drug was observed in the printing temperature, and no change in tacrolimus crystallinity was detected based on TGA and DSC analyses, respectively. The novel, sTable 3D-printed tablet, fabricated using controllable additive manufacturing, can quickly provide tailored dosing with specific kinetic release for personalized medicine at the point-of-care.

Metal organic framework-loaded polyethersulfone/polyacrylonitrile photocatalytic nanofibrous membranes under visible light irradiation for the removal of Cr(vi) and phenol from water.

In this work, various amounts of the UiO-66-NH2 and UiO-66-NH2/TiO2 MOFs have been loaded into polyacrylonitrile (PAN) nanofibers supported on polyethersulfone (PES). The visible light irradiation was used to investigate the influence of pH (2-10), initial concentration (10-500 mg L-1), and time (5-240 min) on the removal efficiency of phenol and Cr(vi) in the presence of MOFs. The reaction time: 120 min, catalyst dosage: 0.5 g L-1, pH: 2 for Cr(vi) ions and pH: 3 for phenol molecules were optimum to degrade phenol and to reduce Cr(vi) ions. The characterization of the produced samples was performed using X-ray diffraction, ultraviolet-visible diffuse reflectance spectroscopy, scanning electron microscopy, and Brunauer-Emmett-Teller analysis. The capability of synthesized photocatalytic membranes was investigated for the removal of phenol and Cr(vi) ions from water. The water flux, Cr(vi) and phenol solutions fluxes and their rejection percentages were evaluated under pressure of 2 bar in the presence of visible light irradiation and in the dark. The best performance of the synthesized nanofibers was obtained for UiO-66-NH2/TiO2 MOF 5 wt% loaded-PES/PAN nanofibrous membranes at temperature of 25 °C and pH of 3. Results demonstrated the high capability of MOFs-loaded nanofibrous membranes for the removal of various contaminants such as Cr(vi) ions and phenol molecules from water.

Magnetic biosensors for identification of SARS-CoV-2, Influenza, HIV, and Ebola viruses: a review.

Infectious diseases such as novel coronavirus (SARS-CoV-2), Influenza, HIV, Ebola, etc kill many people around the world every year (SARS-CoV-2 in 2019, Ebola in 2013, HIV in 1980, Influenza in 1918). For example, SARS-CoV-2 has plagued higher than 317 000 000 people around the world from December 2019 to January 13, 2022. Some infectious diseases do not yet have not a proper vaccine, drug, therapeutic, and/or detection method, which makes rapid identification and definitive treatments the main challenges. Different device techniques have been used to detect infectious diseases. However, in recent years, magnetic materials have emerged as active sensors/biosensors for detecting viral, bacterial, and plasmids agents. In this review, the recent applications of magnetic materials in biosensors for infectious viruses detection have been discussed. Also, this work addresses the future trends and perspectives of magnetic biosensors.

Paclitaxel-loaded liposome-incorporated chitosan (core)/poly(ε-caprolactone)/chitosan (shell) nanofibers for the treatment of breast cancer.

Liposomes and nanofibers have been introduced as effective drug delivery systems of anticancer drugs. The performance of chitosan (core)/poly(ε-caprolactone) (PCL)/paclitaxel simple nanofibers, chitosan/paclitaxel (core)/PCL/chitosan (shell) nanofibers and paclitaxel-loaded liposome-incorporated chitosan (core)/PCL-chitosan (shell) nanofibers was investigated for the controlled release of paclitaxel and the treatment of breast cancer. The synthesized formulations were characterized using polydispersity index, dynamic light scattering, zeta potential, scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared analysis. The sustained release of paclitaxel from liposome-loaded nanofibers was achieved within 30 days. The release data was best described using Korsmeyer-Peppas pharmacokinetic model. The cell viabilities of synthesized nanofibrous samples were higher than 98 % ± 1 % toward L929 normal cells after 168 h. The maximum cytotoxicity against MCF-7 breast cancer cells was 85 % ± 2.5 % using liposome-loaded core-shell nanofibers. The in vivo results indicated the reduction of tumor weight from 1.35 ± 0.15 g to 0.65 ± 0.05 g using liposome-loaded core-shell nanofibers and its increasing from 1.35 ± 0.15 g to 3.2 ± 0.2 g using pure core-shell nanofibers. The three-stage drug release behavior of paclitaxel-loaded liposome-incorporated core-shell nanofibers and the high in vivo tumor efficiency suggested the development of these formulations for cancer treatment in the future.

PVA/κ-carrageenan/Au/camptothecin/pegylated-polyurethane/paclitaxel nanofibers against lung cancer treatment.

Gold nanoparticles, paclitaxel (PTX), and camptothecin (CMPT) were loaded into the PVA/κ-carrageenan/pegylated-PU composite and core-shell nanofibers prepared by two-nozzle and coaxial electrospinning methods. The capability of composite and core-shell nanofibers was investigated for the targeted delivery of anticancer drugs in lung cancer treatment. In vitro and in vivo release of PTX and CMPT were investigated to find the release mechanism from nanofibers compared to direct administration of pristine PTX and CMPT. The mean fiber diameter for composite and core-shell nanofibers with shell feeding rates of 0.3, 0.5, and 0.7 mL h-1 was about 225, 330, 520, and 640 nm, respectively. In vivo release studies indicated that the blood concentration of CMPT and PTX for rats fed with core-shell nanofibers reached the highest values of 26.8 ± 0.04 µg mL-1, and 26.5 ± 0.05 µg mL-1 in 36 h, and 24 h and reduced slowly within 84 h, and 48 h, respectively. The maximum cytotoxicity was 75% in the presence PVA/κ-carrageenan/CMPT/Au/pegylated-PU/PTX core-shell nanofibers. In vivo antitumor activity results confirmed the synergic effect of Au, CMPT and PTX anticancer drugs on the reduction of tumor volume without change in mouse weight by the PVA/κ-carrageenan/CMPT/Au/pegylated PU/PTX core-shell nanofibers. The obtained results indicated that the simultaneous loading of CMPT and PTX anticancer drugs and Au nanoparticles is more beneficial for lung cancer treatment.

Preparation of colloidal nanoparticles PVA-PHEMA from hydrolysis of copolymers of PVAc-PHEMA as anticancer drug carriers.

The novel pH-responsive polymer nanoparticles have been widely used for drug delivery and cancer therapy. The pH-sensitive nanoparticles include chemical structures that can accept or donate protons in response to an environmental pH change. Polybases which mostly contain alkaline groups such as amines and hydroxy, accept protons at low pH and are neutral at higher pH values. This study aimed to prepare pH-sensitive colloidal amphiphilic poly(vinyl alcohol-2-hydroxyethyl methacrylate) (PVA-PHEMA) copolymers in cancer therapy applications. For this purpose, poly(vinyl acetate-2-hydroxyethyl methacrylate) (PVAc-PHEMA) copolymer nanoparticles were synthesized in different polymerization medium fractions from water and methanol and different monomer feed concentration. Then acetate groups were hydrolyzed, and the PHEMA-PVA nanoparticles were synthesized. The nanoparticles were further characterized using dynamic light scattering, Fourier transform infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis to identify the structural and morphological changes. The Methotrexate (MTX) was loaded into the nanoparticles, and drug release kinetics were evaluated. The results confirmed that PHEMA-PVA copolymeric nanoparticles could be favorably used in cancer therapy.

Adsorption, and controlled release of doxorubicin from cellulose acetate/polyurethane/multi-walled carbon nanotubes composite nanofibers.

The cellulose acetate (CA)/poly (ε-caprolactone diol)/poly (tetramethylene ether) glycol-polyurethane (PCL-Diol/PTMG-PU)/multi-walled carbon nanotubes (MWCNTs) composite nanofibers were prepared via two-nozzle electrospinning on both counter sides of the collector. The performance of synthesized composite nanofibers was investigated as an environmental application and anticancer delivery system for the adsorption/release of doxorubicin (DOX). The synergic effect of MWCNTs and DOX incorporated into the nanofibers was investigated against LNCaP prostate cancer cells. The status of MWCNTs and DOX in composite nanofibers was demonstrated by SEM, FTIR and UV-vis determinations. The adsorption tests using nanofibrous adsorbent toward DOX sorption was evaluated under various DOX initial concentrations (100-2000 mg l-1), adsorption times (5-120 min), and pH values (pH:2-9). Due to the fitting of isotherm and kinetic data with Redlich-Peterson and pseudo-second order models, both chemisorption and surface adsorption of DOX molecules mechanisms have been predicted. The drug release from both nanofibers and MWCNTs-loaded nanofibers was compared. The better drug sustained release profiles verified in the presence of composite nanofibers. LNCaP prostate cancer and L929 normal cells were treated to investigate the cytotoxicity and compatibility of synthesized composite nanofibers. The apoptosis/necrosis of hybrid nanofibers and MWCNTs loaded-nanofibers was investigated. The obtained results demonstrated the synergic effects of MWCNTs and DOX loaded-nanofibers on the LNCaP prostate cancer cells death.

Biosensors and nanotechnology for cancer diagnosis (lung and bronchus, breast, prostate, and colon): a systematic review.

The second cause of death in the world has been reported to be cancer, and it has been on the rise in recent years. As a result of the difficulties of cancer detection and its treatment, the survival rate of patients is unclear. The early detection of cancer is an important issue for its therapy. Cancer detection based on biomarkers may effectively enhance the early detection and subsequent treatment. Nanomaterial-based nanobiosensors for cancer biomarkers are excellent tools for the molecular detection and diagnosis of disease. This review reports the latest advancement and attainment in applying nanoparticles to the detection of cancer biomarkers. In this paper, the recent advances in the application of common nanomaterials like graphene, carbon nanotubes, Au, Ag, Pt, and Fe3O4together with newly emerged nanoparticles such as quantum dots, upconversion nanoparticles, inorganics (ZnO, MoS2), and metal-organic frameworks for the diagnosis of biomarkers related to lung, prostate, breast, and colon cancer are highlighted. Finally, the challenges, outlook, and closing remarks are given.

Electrospun gold nanorods/graphene oxide loaded-core-shell nanofibers for local delivery of paclitaxel against lung cancer during photo-chemotherapy method.

The combinations of photothermal therapy (PTT) and chemotherapy (CHT) have attracted increasing attention for cancer therapy. In the present study, paclitaxel as an anticancer drug and graphene oxide/gold nanorods (GO/Au NRs) were simultaneously loaded into the poly (tetramethylene ether) glycol based-polyurethane (PTMG-PU) (core)/chitosan (shell) nanofibers prepared by the coaxial electrospinning method. The potential of the synthesized nanofiber as a pH/temperature dual responsive carrier was investigated for the controlled release of paclitaxel against A549 lung cancer during PTT/CHT combined method. The synthesized core-shell nanofibers were characterized using SEM, TEM and XRD analysis. The drug encapsulation efficiency, drug release and kinetic studies were carried out. The compatibility of the synthesized core-shell nanofibers was also investigated. The cell viability of the synthesized nanofibers treated with A549 lung cancer cells was investigated under alone CHT, alone PTT and PTT/CHT method. The in vivo studies indicated that the PTT/CHT method demonstrated an optimal therapeutic effect on tumor inhibition without change in body weight. The obtained results demonstrated that the synthesized core-shell nanofibers would be used for lung cancer treatment under NIR irradiation in the future.

A review on the applications of electrospun chitosan nanofibers for the cancer treatment.

In recent years, the incidence of cancer is increasing every day due to poor quality of life (industrialization of life). Therefore, the treatment of cancer has received much attention from therapists. So far, many anticancer drugs have been used to treat cancer patents. However, the direct use of the anticancer drugs has the adverse side effects for patents and several limitations to treat process. Natural chitosan nanofibers prepared by electrospinning method have unique properties such as high surface area, high porosity, suitable mechanical properties, nontoxicity, biocompatibility, biodegradability, biorenewable, low immunogenicity, better clinical functionality, analogue to extracellular model, and easy production in large scale. Therefore, this bio-polymer is a very suitable case to deliver of the anti-cancer drugs to treat cancer patents. In this review summarizes the electrospinning synthesis of chitosan and its therapeutic application for the various cancer treatment.

Magnetic bioactive glasses/Cisplatin loaded-chitosan (CS)-grafted- poly (ε-caprolactone) nanofibers against bone cancer treatment.

The bioactive glasses (BGs)/Cisplatin and magnetic bioactive glasses (MBGs)/Cisplatin were doped into the chitosan (CS)-grafted- poly (ε-caprolactone) (PCL) nanofibers for controlled release of Cisplatin under various pH values and temperatures. The simultaneous effect of chemotherapy and hyperthermia was investigated against MG-63 osteosarcoma cells by treating of cells with Cs-g-PCL/MBGs/Cisplatin under an alternating magnetic field. The synthesized nanofibers were characterized using XRD, FTIR, 1H NMR, SEM, and EDX analysis. The bioactivity, and drug loading efficiency of fibers were investigated. There was no initial burst release of Cisplatin from BGs/Cisplatin and MBGs/Cisplatin loaded Cs-g-PCL/MBGs nanofibers and the Cisplatin release rate was accelerated under pH of 5.5 and temperature of 43 °C compared with physiological condition. The apoptotic/necrotic effect indicated that 100 µg mL-1 nanofibers was optimum for killing of MG-63 cells. The future researches could be focused on the application of nanofibers as an implantable device next to a bone tumor for bone cancer therapy in vivo.

Simultaneous linear release of folic acid and doxorubicin from ethyl cellulose/chitosan/g-C3 N4 /MoS2 core-shell nanofibers and its anticancer properties.

The folic acid (FA) and doxorubicin (DOX) have been doped into the g-C3 N4 /MoS2 incorporated-chitosan/ethyl cellulose (EC) core-shell nanofibers for targeted delivery of FA and DOX against HeLa and MCF-7 cell lines. The g-C3 N4 /MoS2 nanosheets and core-shell nanofibers were characterized using Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and UV-Vis tests. The drug loading factor, the degradation rate, and the DOX and FA release behavior from core-shell nanofibers have been investigated. The pharmacokinetic results revealed the linear release with non-Fickian diffusion of the both anticancer drugs from nanofibers during 7 days. The DAPI staining and MTT assays of the nanofibers immersed in MCF-7 and HeLa cell lines were studied to determine the potential of DOX and FA doped-core-shell nanofibrous matrix for MCF-7 and HeLa cells death in vitro. The maximum MCF-7 and HeLa cells death percentages were found to be 89 and 85%, respectively, using EC/chitosan/g-C3 N4 /MoS2 /DOX/FA core-shell nanofibers after 7 days. The high activity of g-C3 N4 /MoS2 /DOX/FA loaded-core-shell nanofibers for studied cancer cells killing was achieved.

Synthesis of PLGA/chitosan/zeolites and PLGA/chitosan/metal organic frameworks nanofibers for targeted delivery of Paclitaxel toward prostate cancer cells death.

In the present study, the various zeolites including hydrophilic Y zeolite, hydrophobic ZSM-5 zeolite and metal organic frameworks (MOFs) including MIL-101 and ZIF-8 were incorporated into the PLGA/chitosan nanofibers for controlled release of Paclitaxel anticancer drug against prostate cancer in vitro and in vivo. The synthesized nanoparticles and nanofibers were characterized using FTIR, XRD, SEM, BET and water contact angle analysis. The drug loading efficiency of nanofibers containing zeolites and MOFs indicated that the MOFs were more useful compared with zeolites for higher loading of Paclitaxel molecules. The Paclitaxel release behavior from nanofibers containing zeolites and MOFs were also examined. The MTT assay and DAPI staining analysis were used to determine the cytotoxicity and apoptosis effect of nanofibers containing Paclitaxel against LNCaP prostate cancer cell lines. The tumor inhibition rate in vivo was carried out to obtain the optimum nanofibrous formulation with maximum cell death percentage and tumor inhibition rate. The obtained results revealed the better activity of MOFs compared nanozeolites for higher loading of Paclitaxel drug into the nanoparticles and a more sustained release of drug from nanofibers containing MOFs.

Far-reaching advances in the role of carbon nanotubes in cancer therapy.

Cancer includes a group of diseases involving unregulated cell growth with the potential to invade or expand to other parts of the body, resulting in an estimate of 9.6 million deaths worldwide in 2018. Manifold studies have been conducted to design more efficacious techniques for cancer therapy due to the inadequacy of conventional treatments including chemotherapy, surgery, and radiation therapy. With the advances in the biomedical applications of nanotechnology-based systems, nanomaterials have gained increasing attention as promising vehicles for targeted cancer therapy and optimizing treatment outcomes. Owing to their outstanding thermal, electrical, optical and chemical properties, carbon nanotubes (CNTs) have been profoundly studied to explore the various perspectives of their application in cancer treatment. The current study aims to review the role of CNTs whether as a carrier or mediator in cancer treatment for enhancing the efficacy as well as the specificity of therapy and reducing adverse side effects. This comprehensive review indicates that CNTs have the capability to be the next generation nanomaterials to actualize noninvasive targeted eradication of tumors. However, further studies are needed to evaluate the consequences of their biomedical application before the transition into clinical trials, since possible adverse effects of CNTs on biological systems have not been clearly understood.

Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.

In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method. The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis. The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C. The effect of hyperthermia on the release rate of TMZ and PTX from magnetic nanofibers was investigated. An alternating magnetic field could induce the mild hyperthermia (43 °C) for the cells treated with magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min. The release data were best described by the non-Fickian diffusion of Korsmeyer-Peppas equation. The cell viability, flowcytometry and Bcl-2, Bax expression levels were investigated to obtain the optimum nanofibrous carrier for apoptosis of U-87 MG cells in vitro. The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.

UiO-66 metal organic framework nanoparticles loaded carboxymethyl chitosan/poly ethylene oxide/polyurethane core-shell nanofibers for controlled release of doxorubicin and folic acid.

Doxorubicin (DOX) and folic acid (FA) were incorporated into the UiO-66 metal organic framework (MOF) and following were loaded into the carboxymethyl chitosan/poly ethylene oxide (PEO)/polyurethane core-shell nanofibers for controlled release of DOX and FA toward MCF-7 cells death. The synthesized nanocarriers were characterized using TEM, XRD, and SEM analysis. The drug loading efficiency and release profiles of DOX/MOF and FA/MOF from synthesized nanofibers have been investigated. The fitting of kinetic data by the pharmacokinetic models demonstrated the non-Fickian diffusion from nanofibers and Fickian diffusion from core-shell fibers. The cytotoxicity of synthesized nanofibers toward MCF-7 cancer cells was evaluated using DAPI staining, MTT assay and flow cytometry tests to investigate the simultaneous use of DOX and FA in the nanofibrous matrix for MCF-7 cells death in vitro. The maximum cell death using DOX-FA loaded-core-shell fibers produced by coaxial electrospinning method under 0.3, 0.5 and 0.8 mLh-1 shell flow rates were found to be 82 ± 0.7, 83 ± 0.5 and 87 ± 0.5% after 168, 240 and 240 h, respectively. The cytotoxicity results indicated that the co-delivery of DOX and FA into the core-shell fibers could be widely used for various cancers treatment.

Synthesis of magnetic gold coated poly (ε-caprolactonediol) based polyurethane/poly(N-isopropylacrylamide)-grafted-chitosan core-shell nanofibers for controlled release of paclitaxel and 5-FU.

The poly (ε-caprolactonediol) based polyurethane (PCL-Diol-b-PU)/poly(N-isopropylacrylamide)-grafted-chitosan (PNIPAAm-g-chitosan) core-shell nanofibers were synthesized via coaxial electrospinning process. Paclitaxel and 5-FU anticancer drugs were incorporated into the core of nanofibers. The nanofibers surface was coated using magnetic gold nanoparticles and the potential of synthesized nanofibers was investigated for the sustained release of paclitaxel and 5-FU toward 4T1 breast cancer cells death in vitro and in vivo. The synthesized magnetic nanoparticles were characterized using SEM, TEM, XRD and DLS analysis. The surface morphology of nanofibers was studied under various applied voltage and different shell flow rates. The paclitaxel and 5-FU release profiles from nanofibers were examined under acidic and physiological pH. The maximum 4T1 cell killing was found to be 78% using magnetic gold coated-nanofibers in the presence of external magnetic field. The SEM images after incubation of nanofibers in 4T1 breast cancer cells indicated the well adhesion of cells on the nanofibers surface. The in vivo studies showed that the tumor volume did not change during 10 days. The minimum increase in tumor volume was obtained using paclitaxel and 5-FU loaded-nanofibers coated by the magnetic gold nanoparticles. The obtained results demonstrated the high therapeutic efficiency of synthesized nanofibrous carrier toward breast cancer treatment.