Role of Immunohistochemistry in the Diagnosis of Leptospirosis in Neotropical Primates

Background: Leptospirosis is considered the most widespread zoonosis worldwide, occurring more frequently in tropical and developing regions. The aim of the present study was to detect the presence of Leptospira spp. in different primate tissues, using immunohistochemical (IHC) assays, taking advantage of the considerable number of necropsies compatible with a diagnosis of leptospirosis in neotropical primates at the Animal Pathology Laboratory (APL) of the University of Passo Fundo (UPF) in the northern region of Rio Grande do Sul.Materials, Methods & Results: Paraffin-embedded primate tissue samples were selected from necropsy examinations and subjected to IHC. The streptavidin-biotin-peroxidase method was used with diaminobenzidine chromogen (DAB) to verify immunostaining. Of the101 primates tested for Leptospira spp., 51.48% were positive; taining was distributed between lung (76.92%), liver (44.23%), and kidney (32.69%) tissue. Analysis of the combined anatomopathological verification data of the studied organs revealed a high frequency of lesions commonly observed in the tissues of animals exposed to the pathogen. For complementary diagnosis, an anti-Leptospira spp. antibody test was performed in primates at the UPF-Zoo, from which a population of the necropsied animals originated. The microscopic agglutination test (MAT) was utilized, which demonstrated 90.47% positi

Role of Immunohistochemistry in the Diagnosis of Leptospirosis in Neotropical Primates

Background: Leptospirosis is considered the most widespread zoonosis worldwide, occurring more frequently in tropical and developing regions. The aim of the present study was to detect the presence of Leptospira spp. in different primate tissues, using immunohistochemical (IHC) assays, taking advantage of the considerable number of necropsies compatible with a diagnosis of leptospirosis in neotropical primates at the Animal Pathology Laboratory (APL) of the University of Passo Fundo (UPF) in the northern region of Rio Grande do Sul.Materials, Methods & Results: Paraffin-embedded primate tissue samples were selected from necropsy examinations and subjected to IHC. The streptavidin-biotin-peroxidase method was used with diaminobenzidine chromogen (DAB) to verify immunostaining. Of the101 primates tested for Leptospira spp., 51.48% were positive; taining was distributed between lung (76.92%), liver (44.23%), and kidney (32.69%) tissue. Analysis of the combined anatomopathological verification data of the studied organs revealed a high frequency of lesions commonly observed in the tissues of animals exposed to the pathogen. For complementary diagnosis, an anti-Leptospira spp. antibody test was performed in primates at the UPF-Zoo, from which a population of the necropsied animals originated. The microscopic agglutination test (MAT) was utilized, which demonstrated 90.47% positi

Role of Immunohistochemistry in the Diagnosis of Leptospirosis in Neotropical Primates

Background: Leptospirosis is considered the most widespread zoonosis worldwide, occurring more frequently in tropical and developing regions. The aim of the present study was to detect the presence of Leptospira spp. in different primate tissues, using immunohistochemical (IHC) assays, taking advantage of the considerable number of necropsies compatible with a diagnosis of leptospirosis in neotropical primates at the Animal Pathology Laboratory (APL) of the University of Passo Fundo (UPF) in the northern region of Rio Grande do Sul.Materials, Methods & Results: Paraffin-embedded primate tissue samples were selected from necropsy examinations and subjected to IHC. The streptavidin-biotin-peroxidase method was used with diaminobenzidine chromogen (DAB) to verify immunostaining. Of the101 primates tested for Leptospira spp., 51.48% were positive; taining was distributed between lung (76.92%), liver (44.23%), and kidney (32.69%) tissue. Analysis of the combined anatomopathological verification data of the studied organs revealed a high frequency of lesions commonly observed in the tissues of animals exposed to the pathogen. For complementary diagnosis, an anti-Leptospira spp. antibody test was performed in primates at the UPF-Zoo, from which a population of the necropsied animals originated. The microscopic agglutination test (MAT) was utilized, which demonstrated 90.47% positi

Role of Immunohistochemistry in the Diagnosis of Leptospirosis in Neotropical Primates

Background: Leptospirosis is considered the most widespread zoonosis worldwide, occurring more frequently in tropical and developing regions. The aim of the present study was to detect the presence of Leptospira spp. in different primate tissues, using immunohistochemical (IHC) assays, taking advantage of the considerable number of necropsies compatible with a diagnosis of leptospirosis in neotropical primates at the Animal Pathology Laboratory (APL) of the University of Passo Fundo (UPF) in the northern region of Rio Grande do Sul.Materials, Methods & Results: Paraffin-embedded primate tissue samples were selected from necropsy examinations and subjected to IHC. The streptavidin-biotin-peroxidase method was used with diaminobenzidine chromogen (DAB) to verify immunostaining. Of the101 primates tested for Leptospira spp., 51.48% were positive; taining was distributed between lung (76.92%), liver (44.23%), and kidney (32.69%) tissue. Analysis of the combined anatomopathological verification data of the studied organs revealed a high frequency of lesions commonly observed in the tissues of animals exposed to the pathogen. For complementary diagnosis, an anti-Leptospira spp. antibody test was performed in primates at the UPF-Zoo, from which a population of the necropsied animals originated. The microscopic agglutination test (MAT) was utilized, which demonstrated 90.47% positi

Role of Immunohistochemistry in the Diagnosis of Leptospirosis in Neotropical Primates

Background: Leptospirosis is considered the most widespread zoonosis worldwide, occurring more frequently in tropical and developing regions. The aim of the present study was to detect the presence of Leptospira spp. in different primate tissues, using immunohistochemical (IHC) assays, taking advantage of the considerable number of necropsies compatible with a diagnosis of leptospirosis in neotropical primates at the Animal Pathology Laboratory (APL) of the University of Passo Fundo (UPF) in the northern region of Rio Grande do Sul.Materials, Methods & Results: Paraffin-embedded primate tissue samples were selected from necropsy examinations and subjected to IHC. The streptavidin-biotin-peroxidase method was used with diaminobenzidine chromogen (DAB) to verify immunostaining. Of the101 primates tested for Leptospira spp., 51.48% were positive; taining was distributed between lung (76.92%), liver (44.23%), and kidney (32.69%) tissue. Analysis of the combined anatomopathological verification data of the studied organs revealed a high frequency of lesions commonly observed in the tissues of animals exposed to the pathogen. For complementary diagnosis, an anti-Leptospira spp. antibody test was performed in primates at the UPF-Zoo, from which a population of the necropsied animals originated. The microscopic agglutination test (MAT) was utilized, which demonstrated 90.47% positi

Role of Immunohistochemistry in the Diagnosis of Leptospirosis in Neotropical Primates

Background: Leptospirosis is considered the most widespread zoonosis worldwide, occurring more frequently in tropical and developing regions. The aim of the present study was to detect the presence of Leptospira spp. in different primate tissues, using immunohistochemical (IHC) assays, taking advantage of the considerable number of necropsies compatible with a diagnosis of leptospirosis in neotropical primates at the Animal Pathology Laboratory (APL) of the University of Passo Fundo (UPF) in the northern region of Rio Grande do Sul.Materials, Methods & Results: Paraffin-embedded primate tissue samples were selected from necropsy examinations and subjected to IHC. The streptavidin-biotin-peroxidase method was used with diaminobenzidine chromogen (DAB) to verify immunostaining. Of the101 primates tested for Leptospira spp., 51.48% were positive; taining was distributed between lung (76.92%), liver (44.23%), and kidney (32.69%) tissue. Analysis of the combined anatomopathological verification data of the studied organs revealed a high frequency of lesions commonly observed in the tissues of animals exposed to the pathogen. For complementary diagnosis, an anti-Leptospira spp. antibody test was performed in primates at the UPF-Zoo, from which a population of the necropsied animals originated. The microscopic agglutination test (MAT) was utilized, which demonstrated 90.47% positi

Production of meat and characteristics of lamb carcass non-castrated, castrated and induced to cryptorchidism / Produção de carne e caracteristicas da carcaça de cordeiros não castrados, castrados e induzidos ao criptorquidismo

The characteristics of lamb meat are influenced by a series of factors, among which the testicular hormone production. In order to estimate the influence on meat characteristics, 24 Suffolk, Ile de France, Santa Inês and Texel crossbred lambs male, in a completely casualised delineation, with three treatments and eight repetitions. All animals were managed in the same way, pasture-raised and fed on native grass, in a Voisin system. The experiment treatments were constituted of non-castrated, castrated and cryptorchid animals. Live weight measurements were recorded at birth, once a month to assess body development and before slaughter. The warm carcass weight at slaughter and the 24 hours post-slaughter weight of the carcass cooled in refrigeration chamber at 5ºC were recorded.  Also, the weights of the variables related to the carcass were obtained. The results were analyzed using GLM Procedure and the means compared by Tukeys test at the 5% significance level. No differences in production were found in the components and in the characteristics of the ovine meat and carcass of non-castrated, castrated and cryptorchid lambs raised on pasture land, fed on native grass and slaughtered young.   

As características da carne de cordeiro são influenciadas por uma série de fatores, entre os quais a produção hormonal a nível testicular. Para estimar a influência sobre a produção e as características da carne, foram utilizados 24 cordeiros machos cruzas entre as raças Suffolk, Ile de France, Santa Inês e Texel em um delineamento completamente casualizado com três tratamentos e oito repetições. Todos os animais foram submetidos ao mesmo manejo e criados sobre pastagens de campo nativo em um sistema Voisin. Os tratamentos foram constituídos de animais não castrados, castrados e induzidos ao criptorquidismo. O peso vivo foi obtido ao nascimento e posteriormente, uma vez por mês para o controle do desenvolvimento corporal, e antes do abate. Após o abate foi tomado o peso da carcaça quente e 24 horas após, o peso da carcaça resfriada em câmara fria a 5ºC. Também foram obtidos os pesos das variáveis relacionadas à carcaça. Os resultados foram analisados através do GLM Procedure e as médias comparadas através do Teste de Tukey a 5% de significância. Não houve diferenças na produção, nos componentes e nas características da carne e da carcaça ovina produzidas por cordeiros não castrados, castrados e induzidos ao criptorquidismo criados em condições de pastagem nativa e abatidos jovens.

Nefrectomia videolaparoscópica para tratamento da dioctofimose em um cão

A dioctofimose é uma afecção pouco comum em cães, causada pelo  Dioctophyma renale, que ocorre com maior frequência em animais errantes. Quando um único rim é parasitado, os pacientes podem não apresentar sinais clínicos devido à compensação pelo rim contralateral. Não havendo terapia clínica efetiva para a dioctofimose, o tratamento indicado é a nefrotomia ou nefrectomia, na dependência da gravidade da lesão. Foi atendido no Hospital Veterinário da Universidade de Passo Fundo (UPF) um canino, fêmea, castrado, sem raça definida, com aproximadamente um ano e pesando 12 kg, proveniente de um abrigo de animais de rua da cidade de Passo Fundo (RS), apresentando hematúria e emagrecimento progressivo há 30 dias. Através dos exames complementares realizados, foi constatado parasitismo do rim direito por D. renale. O presente relato descreve o emprego alternativo da nefrectomia videolaparoscópica como um método seguro e efetivo para o tratamento de dioctofimose em um cão e os meios utilizados para o diagnóstico.

Nefrectomia videolaparoscópica para tratamento da dioctofimose em um cão

A dioctofimose é uma afecção pouco comum em cães, causada pelo  Dioctophyma renale, que ocorre com maior frequência em animais errantes. Quando um único rim é parasitado, os pacientes podem não apresentar sinais clínicos devido à compensação pelo rim contralateral. Não havendo terapia clínica efetiva para a dioctofimose, o tratamento indicado é a nefrotomia ou nefrectomia, na dependência da gravidade da lesão. Foi atendido no Hospital Veterinário da Universidade de Passo Fundo (UPF) um canino, fêmea, castrado, sem raça definida, com aproximadamente um ano e pesando 12 kg, proveniente de um abrigo de animais de rua da cidade de Passo Fundo (RS), apresentando hematúria e emagrecimento progressivo há 30 dias. Através dos exames complementares realizados, foi constatado parasitismo do rim direito por D. renale. O presente relato descreve o emprego alternativo da nefrectomia videolaparoscópica como um método seguro e efetivo para o tratamento de dioctofimose em um cão e os meios utilizados para o diagnóstico.

Comparison of two methods for the control of gastrointestinal nematodes in lambs and ewes / Comparação de dois métodos de controle de nematódeos gastrintestinais em borregas e ovelhas de corte

The objective of this work was to evaluate the control of nematode gastrintestinal on ovine meat breeds in Voisin pasturing system during one grazing year. The experiment was carried out at the Research Center Farm of the Passo Fundo University during the period of June 2005 to July 2006. The animals were kept in 85 pasture blocks with 810 square meters each. In the experiment 42 ewe hoggets and 42 Suffolk, Ile de France purebreed and crosses ewes were subdivided in four groups composed of 21 animals each. The groups had been assigned as G1 (traditional sheep), G2 (traditional young ewes) both treated every 60 days with Levamisole; the groups G3 (Famacha® sheep) and G4 (Famacha® young ewes) were evaluated by the Famacha® method. The following variables were evaluated: live weight and worm egg counts (EPG). In relation to the live weights it was proven that it did not have statistics difference (P>0,05) between ewe hoggets and adult sheep of the traditional groups when compared with the ones of the Famacha® method. Differences in EPG counts during of the year in the ewe hoggets were found it had a significant reduction in the frequency of treatments for the Famacha® method when compared with the traditional method, of 90,39% for ewe hoggets and 90.93% for the sheep, becoming this method a viable alternative.

Este trabalho teve como objetivo avaliar o controle de helmintos gastrintestinais em ovinos de corte em um sistema de pastoreio Voisin em quatro estações do ano. Foi realizado no Centro de Pesquisa Agropecuária da Universidade Passo Fundo no período de junho de 2005 a julho de 2006. Foram utilizados 84 fêmeas das raças Suffolk, Ile de France puras e mestiças as quais foram subdivididas em quatro grupos composto de 21 animais cada. Os grupos foram designados como G1 (ovelha tradicional), G2 (borrega tradicional) tratadas a cada 60 dias com Levamisole; os grupos G3 (ovelha Famacha®), e G4 (borrega Famacha®) foram avaliadas pelo método Famacha®. As variáveis analisadas foram peso vivo e ovos por grama de fezes (OPG). Em relação ao peso vivo não houve diferença estatística (P>0,05) entre borregas e ovelhas dos grupos tradicionais quando comparadas aos do método Famacha®. Ocorreram diferenças no OPG no decorrer das estações do ano para as borregas. Houve uma significativa redução na freqüência dos tratamentos pelo método Famacha® quando comparado ao método tradicional, de 90,39% para as borregas e 90,93% para as ovelhas, tornando este método uma alternativa viável.

Comparison of two methods for the control of gastrointestinal nematodes in lambs and ewes / Comparação de dois métodos de controle de nematódeos gastrintestinais em borregas e ovelhas de corte

The objective of this work was to evaluate the control of nematode gastrintestinal on ovine meat breeds in Voisin pasturing system during one grazing year. The experiment was carried out at the Research Center Farm of the Passo Fundo University during the period of June 2005 to July 2006. The animals were kept in 85 pasture blocks with 810 square meters each. In the experiment 42 ewe hoggets and 42 Suffolk, Ile de France purebreed and crosses ewes were subdivided in four groups composed of 21 animals each. The groups had been assigned as G1 (traditional sheep), G2 (traditional young ewes) both treated every 60 days with Levamisole; the groups G3 (Famacha® sheep) and G4 (Famacha® young ewes) were evaluated by the Famacha® method. The following variables were evaluated: live weight and worm egg counts (EPG). In relation to the live weights it was proven that it did not have statistics difference (P>0,05) between ewe hoggets and adult sheep of the traditional groups when compared with the ones of the Famacha® method. Differences in EPG counts during of the year in the ewe hoggets were found it had a significant reduction in the frequency of treatments for the Famacha® method when compared with the traditional method, of 90,39% for ewe hoggets and 90.93% for the sheep, becoming this method a viable alternative.

Este trabalho teve como objetivo avaliar o controle de helmintos gastrintestinais em ovinos de corte em um sistema de pastoreio Voisin em quatro estações do ano. Foi realizado no Centro de Pesquisa Agropecuária da Universidade Passo Fundo no período de junho de 2005 a julho de 2006. Foram utilizados 84 fêmeas das raças Suffolk, Ile de France puras e mestiças as quais foram subdivididas em quatro grupos composto de 21 animais cada. Os grupos foram designados como G1 (ovelha tradicional), G2 (borrega tradicional) tratadas a cada 60 dias com Levamisole; os grupos G3 (ovelha Famacha®), e G4 (borrega Famacha®) foram avaliadas pelo método Famacha®. As variáveis analisadas foram peso vivo e ovos por grama de fezes (OPG). Em relação ao peso vivo não houve diferença estatística (P>0,05) entre borregas e ovelhas dos grupos tradicionais quando comparadas aos do método Famacha®. Ocorreram diferenças no OPG no decorrer das estações do ano para as borregas. Houve uma significativa redução na freqüência dos tratamentos pelo método Famacha® quando comparado ao método tradicional, de 90,39% para as borregas e 90,93% para as ovelhas, tornando este método uma alternativa viável.