[The application of the immunohistological method for the detection of ABO antigens in cellular elements].

This paper was designed to illustrate the advantages of immunohistological methods over those traditionally employed in this country for the study of material evidence. A method for the identification of ABO antigens in cellular elements based on the use of Diagnostic BioSystems-Universal HPP kits is described. The reaction is specific and as sensitive as the immunofluorescent techniques. The stained preparations can be stored during a long period without the loss of their properties. The authors recommend to use the method being considered in forensic medical (specifically, in forensic cytological) practice.

[Possibility of the species identification using blood stains located on the material evidences and bone fragments with the method of solid phase enzyme immunoassay with "IgG general-EIA-BEST" kit and human immunoglobulin G].

The authors give the comparative analysis of Russian and foreign forensic medical methods of species character identification of the blood from the stains on the material evidences and bone fragments. It is shown that for this purpose it is feasible to apply human immunoglobulin G (IgG) and solid phase enzyme immunoassay (EIA) with the kit "IgG general-EIA-BEST". In comparison with the methods used in Russia this method is more sensitive, convenient for objective registration and computer processing. The results of experiments shown that it is possible to use the kit "IgG general-EIA-BEST" in forensic medicine for the species character identification of the blood from the stains on the material evidences and bone fragments.

[Identification of sperm on the spots located on material evidences using solid phase enzyme immunoassay with human prostate-specific antigen].

It is shown that for verification of human sperm presence on the spots on material evidence it is feasible to apply solid phase enzyme immunoassay (EIA) with the kit "PSA-EIA-BEST T-8458" which is used in clinical practice for diagnosis of prostatic cancer and some other diseases. The method is specific and highly sensitive, convenient for objective registration and computer processing. It is also cost-effective and provides not only significant identification of sperm on the exhibits but also its human origin in complete absence of spermatozoa on the spot.

[The detection of ABO system antigens in bone, nail and tooth fragments]. / Vyiavlenie antigenov sistemy ABO v fragmentakh kostei, nogtei i zubov.

Fragments of the bones, nails, and teeth were examined in 12 forensic biological departments of bureaus for forensic medical expert evaluation of the Russian Federation. A total of 790 experiments were carried out with 145 bones, 5 teeth, and nails from 12 cadavers. In the overwhelming majority of cases the experiments were carried out with control investigations of blood, hair, salivary, and sweat samples. Modifications of the absorption-elution method were used to detect the AB0 antigens, these modifications involving all stages of the test (fixation, fat extraction, medium for elution, reagents, time factors in absorption and elution, etc.). Analysis of the data permitted the authors to make some conclusions and offer recommendations for practical biological experts.