Stable partial nitritation for low-strength wastewater at low temperature in an aerobic granular reactor.

Partial nitritation for a low-strength wastewater at low temperature was stably achieved in an aerobic granular reactor. A bench-scale granular sludge bioreactor was operated in continuous mode treating an influent of 70 mg N-NH4(+) L(-1) to mimic pretreated municipal nitrogenous wastewater and the temperature was progressively decreased from 30 to 12.5 °C. A suitable effluent nitrite to ammonium concentrations ratio to a subsequent anammox reactor was maintained stable during 300 days at 12.5 °C. The average applied nitrogen loading rate at 12.5 °C was 0.7 ± 0.3 g N L(-1) d(-1), with an effluent nitrate concentration of only 2.5 ± 0.7 mg N-NO3(-) L(-1). The biomass fraction of nitrite-oxidizing bacteria (NOB) in the granular sludge decreased from 19% to only 1% in 6 months of reactor operation at 12.5 °C. Nitrobacter spp. where found as the dominant NOB population, whereas Nitrospira spp. were not detected. Simulations indicated that: (i) NOB would only be effectively repressed when their oxygen half-saturation coefficient was higher than that of ammonia-oxidizing bacteria; and (ii) a lower specific growth rate of NOB was maintained at any point in the biofilm (even at 12.5 °C) due to the bulk ammonium concentration imposed through the control strategy.

Microbial community shifts on an anammox reactor after a temperature shock using 454-pyrosequencing analysis.

To explore the changes in the microbial community structure during the recovery process of an anammox reactor after a temperature shock, the 454-pyrosequencing technique was used. The temperature shock reduced the nitrogen removal rate up to 92% compared to that just before the temperature shock, and it took 70 days to recover a similar nitrogen removal rate to that before the temperature shock (ca. 0.30 g N L(-1) d(-1)). Pyrosequencing results indicated that microbial diversity in the reactor decreased as the reactor progressively recovered from the temperature shock. Anammox bacteria were accounted as 6%, 35% and 46% of total sequence reads in samples taken 13, 45 and 166 days after the temperature shock. These results were in agreement with N-removal performance results and anammox activity measured in the reactor during the recovery process. An anammox specific primer was used to precisely determine the anammox species in the biomass samples.

Closed-loop control of ammonium concentration in nitritation: convenient for reactor operation but also for modeling.

A mathematical biofilm model was developed to describe nitritation in aerobic granular reactor operating in continuous mode. The model includes the automatic closed-loop control of ammonium concentration in the effluent. This is integrated in a ratio control strategy to maintain the proportion between the dissolved oxygen (DO) and the total ammonia nitrogen (TAN) concentrations in the reactor effluent at a desired value. The model was validated with a large set of experimental results previously reported in the literature. The model was used to study the effect of DO and TAN setpoints on the achievement of full nitritation, as well as to establish the appropriate required range of the DO/TAN concentration ratio to be applied. Nitritation at 20 °C was tested experimentally and simulated with the model. Additionally, the importance of controlling the TAN concentration was highlighted with different scenarios, in which periodic disturbances were applied mimicking a poor control situation.