RESUMO
Bone morphogenetic protein (BMP), a member of the transforming growth factor superfamily, is one of the most potent growth factors that stimulate osteoblast differentiation and bone formation. We investigated the effects of recombinant human BMP-2 (rhBMP-2) on osteoblast differentiation and matrix metalloproteinase-1 (MMP-1) production in human bone cells (HBC) isolated from mandibulae of 3 adult patients. rhBMP-2 at concentrations over 50 ng/ml significantly stimulated alkaline phosphatase activity and parathyroid hormone (PTH)-dependent 3', 5'-cyclic adenosine monophosphate accumulation, which are early markers of osteoblast differentiation, in HBCs. rhBMP-2 (500 ng/ml) also enhanced the level of PTH/PTH related-peptide receptor mRNA expression in HBCs. Although neither HBCs untreated nor treated with rhBMP-2 produced measurable amounts of osteocalcin, which is a marker of more mature osteoblasts, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] induced ostocalcin mRNA expression and its protein synthesis in these cells. rhBMP-2 inhibited 1,25(OH)2D3-induced osteocalcin synthesis in HBCs at both the mRNA and protein level. rhBMP-2 also significantly suppressed MMP-1 production and MMP-1 mRNA expression at concentrations over 500 ng/ml. These results suggest that rhBMP-2 exerts anabolic effects on human osteoblastic cells derived from mandibulae by stimulation of osteoblast differentiation and down-regulation of MMP-1 synthesis.
Assuntos
Proteínas Morfogenéticas Ósseas/farmacologia , Inibidores de Metaloproteinases de Matriz , Osteoblastos/efeitos dos fármacos , Osteoblastos/enzimologia , Fator de Crescimento Transformador beta , Adulto , Fosfatase Alcalina/antagonistas & inibidores , Proteína Morfogenética Óssea 2 , Diferenciação Celular/efeitos dos fármacos , Colágeno/metabolismo , Colagenases/biossíntese , AMP Cíclico/biossíntese , Indução Enzimática/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Fibroblastos/enzimologia , Humanos , Mandíbula/citologia , Metaloproteinase 1 da Matriz , Osteocalcina/antagonistas & inibidores , Osteocalcina/biossíntese , Proteínas Recombinantes/farmacologiaRESUMO
The purpose of this study was to determine the effects of a fibrin tissue adhesive material (FAM) on periodontal tissue regeneration. In an in vitro study comparing osteogenic cells with gingival fibroblasts, it was shown that the degradation of FAM adjacent to the osteogenic cells was faster than that adjacent to the gingival fibroblasts. In two in vivo studies in dogs where surgical bony defects were created, it was shown through histometric measurements that in the sites where FAM was applied, more new bone was found than in the control sites. It was concluded that FAM may enhance periodontal tissue regeneration.
