Assuntos
COVID-19 , Hospital Dia , Hospitais Psiquiátricos , Transtornos Mentais/reabilitação , Adulto , Atenção à Saúde , Exercício Físico/psicologia , Feminino , Humanos , Japão , Masculino , Adesão à Medicação , Transtornos Mentais/psicologia , Pessoa de Meia-Idade , Motivação , Transtornos Psicóticos/psicologia , Transtornos Psicóticos/reabilitação , Esquizofrenia/reabilitação , Sono , Estresse Psicológico/psicologia , Exacerbação dos SintomasRESUMO
BACKGROUND: The common marmoset (Callithrix jacchus) is one of the most studied primate model organisms. However, the marmoset genomes available in the public databases are highly fragmented and filled with sequence gaps, hindering research advances related to marmoset genomics and transcriptomics. RESULTS: Here we utilize single-molecule, long-read sequence data to improve and update the existing genome assembly and report a near-complete genome of the common marmoset. The assembly is of 2.79 Gb size, with a contig N50 length of 6.37 Mb and a chromosomal scaffold N50 length of 143.91 Mb, representing the most contiguous and high-quality marmoset genome up to date. Approximately 90% of the assembled genome was represented in contigs longer than 1 Mb, with approximately 104-fold improvement in contiguity over the previously published marmoset genome. More than 98% of the gaps from the previously published genomes were filled successfully, which improved the mapping rates of genomic and transcriptomic data on to the assembled genome. CONCLUSIONS: Altogether the updated, high-quality common marmoset genome assembly provide improvements at various levels over the previous versions of the marmoset genome assemblies. This will allow researchers working on primate genomics to apply the genome more efficiently for their genomic and transcriptomic sequence data.
Assuntos
Callithrix/genética , Mapeamento Cromossômico/métodos , Genoma/genética , Animais , Biologia Computacional/métodos , Mapeamento de Sequências Contíguas/métodos , Genômica/métodos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Alinhamento de SequênciaAssuntos
Púrpura Trombocitopênica Idiopática/tratamento farmacológico , Receptores Fc/uso terapêutico , Receptores de Trombopoetina/agonistas , Proteínas Recombinantes de Fusão/uso terapêutico , Trombopoetina/uso terapêutico , Animais , Doença Crônica , Ensaios Clínicos como Assunto , Humanos , Injeções Subcutâneas , Megacariócitos/efeitos dos fármacos , Camundongos , Ensaios Clínicos Controlados Aleatórios como Assunto , Receptores Fc/administração & dosagem , Receptores Fc/metabolismo , Receptores de Trombopoetina/metabolismo , Proteínas Recombinantes de Fusão/administração & dosagem , Proteínas Recombinantes de Fusão/metabolismo , Proteínas Recombinantes de Fusão/farmacologia , Transdução de Sinais , Células-Tronco/efeitos dos fármacos , Trombopoetina/administração & dosagem , Trombopoetina/metabolismo , Trombopoetina/farmacologiaRESUMO
Molasses of tangerine orange (Citrus unshiu Markovich) is obtained as a waste product in the course of tangerine orange juice production. This molasses is expected to be a useful source of organic compounds such as flavonoids and limonoids. To elucidate a use for this molasses waste, we isolated and identified its organic constituents. Two new flavanonol glycosides were isolated from tangerine orange molasses, along with several flavonoids such as hesperidine, narirutin, eriodictyol, 3',4',5,6,7,8-hexamethoxy-3-O-beta-D-glucopyranosyloxyflavone, and 3',4',5,6,7,8-hexamethoxy- 3-beta-D-[4-O-(3-hydroxy-3-methylglutaloyl)]-glucopyranosyloxyflavone, and limonoids such as limonin, nomilin, and cyclic peptide, citrusin III. The structures of the new flavanonol glycosides were determined as (2R,3R)-7-O-(6-O-alpha-L-rahmnopyranosyl-beta-D-glucopyranosyl)-aromadendrin and 7-O-(6-O-alpha-L-rahmnopyranosyl-beta-D-glucopyranosyl)-3,3',5,7-tetrahydroxy-4'-methoxyflavanone by means of spectral analyses using (1)H-NMR, (13)C-NMR, and 2D-NMR. Of these compounds, flavanone glycoside, hesperidin and narirutin were isolated as the main constituents. Thus, molasses is a promising source of flavonoid glycosides.
