RESUMO
The correlation between changes in activities of glutathione peroxidase and glutathione reductase in heart of rats during development of adrenaline myocarditis and intensity of free radical processes estimated by biochemiluminesce parameters and the content of lipoperoxidation products was demonstrated. The maximal increase of glutathione peroxidase and glutathione reductase activities (in 1.8 and 1.4 times accordingly) was observed t 24 h after the development of the pathological process; this coincided with the maximum intensity of prosesses of free radical oxidation. Using combination of reverse transcriptions with real-time polymerase chain reaction the cardiac mRNA levels of glutathione peroxidase and glutathione reductase genes were determined during the development of adrenaline myocarditis in rats. Analysis of expression of glutathione peroxidase and glutathione reductase genes showed, that the level of this transcripts demonstrated 2,8- and 7,3- increase in rats with adrenaline myocarditis, respectively. Obviously, overexpression of these enzymes can increase the resistance of cardiomyocites to oxidative stress.
Assuntos
Agonistas alfa-Adrenérgicos/efeitos adversos , Epinefrina/efeitos adversos , Radicais Livres/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Glutationa Peroxidase/biossíntese , Glutationa Redutase/biossíntese , Proteínas Musculares/biossíntese , Miocardite/metabolismo , Miocárdio/metabolismo , Agonistas alfa-Adrenérgicos/farmacologia , Animais , Epinefrina/farmacologia , Miocardite/induzido quimicamente , Miocardite/patologia , Miocárdio/patologia , Miócitos Cardíacos/metabolismo , Miócitos Cardíacos/patologia , Estresse Oxidativo/efeitos dos fármacos , RNA Mensageiro/biossíntese , RatosRESUMO
The influence of some guanidine derivatives on the level of brain citrate, brain activities of aconitase and citrate synthase has been investigated in rats subjected to ischemia-reperfusion. Administration of N-[imino(1-piperidinyl)methyl]guanidine and N-[imino(4-morpholinyl)methyl]guanidine resulted in changes of specific activities of aconitase and citrate synthase towards control values. Under these conditions the citrate level considerably decreased versus rats with untreated ishemia-reperfusion. Treatment with these compounds also decreased the degree of DNA fragmentation markedly increased in rats with ischemia-reperfusion.
Assuntos
Aconitato Hidratase/metabolismo , Antioxidantes/farmacologia , Isquemia Encefálica/metabolismo , Encéfalo/efeitos dos fármacos , Citrato (si)-Sintase/metabolismo , Metilguanidina/análogos & derivados , Morfolinas/farmacologia , Piperidinas/farmacologia , Traumatismo por Reperfusão/metabolismo , Animais , Encéfalo/irrigação sanguínea , Encéfalo/metabolismo , Ácido Cítrico/análise , Ácido Cítrico/sangue , Ácido Cítrico/metabolismo , Fragmentação do DNA/efeitos dos fármacos , Relação Dose-Resposta a Droga , Radicais Livres/metabolismo , Masculino , Metilguanidina/farmacologia , Fármacos Neuroprotetores/metabolismo , Fármacos Neuroprotetores/farmacologia , Oxirredução/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , RatosRESUMO
Literature data on magnitosomes, the nanocrystals formed during natural biomineralization have been summarized. Special attention is paid to magnitosome effect on physiological and biochemical processes, impairments of cell homeostasis and development of various pathologies. It is suggested that the increase in quantity and sizes of magnetosomes, spatial rearrangement, and modification of their crystalline substance exert substantial effect on development of pathological processes.
Assuntos
Óxido Ferroso-Férrico/metabolismo , Homeostase , Magnetismo , Nanopartículas , Animais , Bactérias , Óxido Ferroso-Férrico/química , HumanosRESUMO
Development of apoptosis is accompanied by a decrease in transcripts of NADP-isocitrate dehydrogenase (NADP-IDH, E.C. 1.1.1.42.), and also alterations of catalytic properties from the rat liver enzyme in comparison with control. Administration of thioctic acid is increased the level of expression towards normal values. Enzyme preparations NADP-IDH were obtained from rat liver at norm conditions, after introduction of tumor necrosis factor and thioctic acid action under apoptosis. Molecular weight of homogenous preparations of NADP-IDH purified from livers of control and experimental rats was 112 +/- 5.8 kDa, however Km values and pH-optimum changed in apoptosis. Regulation of NADP-IDH activity under effect of some intermediates of the tricarboxylic acid cycle also differed in these groups.
Assuntos
Isocitrato Desidrogenase/metabolismo , Fígado/efeitos dos fármacos , Ácido Tióctico/farmacologia , Fator de Necrose Tumoral alfa/farmacologia , Animais , Apoptose , Fígado/enzimologia , Fígado/patologia , Masculino , RatosRESUMO
Changes of a structural condition of DNA, expression level of the membrane Fas-receptor and of functional activity of the human lymphocytes caspase-3 in dynamics of apoptosis development, induced by influence of UV-light (240-390 nm) in doses 151, 1510 and 3020 J/m2 are studied. It is established, that the UV-irradiation of lymphocytes in doses of 151 and 1510 J/m2 causes realisation of receptor caspase-dependent apoptosis way. The assumption about switching possibility of receptor caspase-dependent way apoptosis on receptor caspase-independent way at increase of a dose of UV-light to 3020 J/m2 is made.