RESUMO
BACKGROUND: MicroRNAs (miRNAs) are non-coding RNAs known to control a broad range of biological functions such as cellular proliferation, differentiation and programmed cell death. Recent reports showed that miRNAs can act as oncogenes or tumor suppressors, thereby, playing an important role in cancer initiation and progression. Moreover, we know that Expressed sequence tags (ESTs) are random single pass sequence reads, which displays the condition/tissue specific transcripts (coding and non-coding) of an organism. METHODS: In the present study, we have applied the bioinformatics approach to identify miRNA from prostate cancer using EST resource and its expressions were analyzed by quantitative reverse transcription PCR (qRT-PCR). RESULTS: Analysis of transcriptomics resource from the LNCaP cells revealed the presence of an EST encoding hsa-miR-3654. Presence of the premature candidate of miR-3654, demonstrates its expression in LNCaP cells. We further indentified that the expression level (Fold Induction) of miR-3654 in LNCaP was higher than the normal and androgen insensitive prostate cancer cell lines (PNT1A, PC-3). CONCLUSION: we have identified the miR-3654 involved in prostate cancer progression using computational approach and hypothesized that the down regulation of miR-3654 could be responsible for a solid tumor to get cancer stem-like cell phenotype. Further studies are required to investigate the molecular mechanisms behind the STAT3 mediated miR-3654 repression and the associated metastasis.
Assuntos
Progressão da Doença , Regulação Neoplásica da Expressão Gênica/genética , MicroRNAs/genética , Células-Tronco Neoplásicas/patologia , Neoplasias da Próstata/genética , Neoplasias da Próstata/patologia , Fator de Transcrição STAT3/genética , Linhagem Celular Tumoral , Biologia Computacional , Etiquetas de Sequências Expressas , Humanos , Masculino , Células-Tronco Neoplásicas/citologia , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
The significant role of microRNAs (miRNAs) in stem cell development has been expansively discussed in recent publications and they are noticed to be involved in post transcriptional regulation of gene expression by its affinity towards the 3'UTR of target mRNA. Since the expression of miR-21 was high in cells that were derived from MSCs, recent evidence indicates that miR-21 plays a vital role in mesenchymal stem cells (MSCs) differentiation. Despite the fact that miR-21 can be considered as a powerful biomarker for MSCs differentiation, the number of studies related to the above scenario is very limited. This review highlights the recent publications related to the importance of miR-21 specifically in differentiation of MSCs.
