Implications of the Mn:ligand ratio for Mn uptake by Glycine max L. plants fertilized with heptagluconate and gluconate complexes.

BACKGROUND: The environmental risk of the application of synthetic chelates has furthered the implementation of biodegradable complexes to correct manganese (Mn)-deficient plants. This study used the biodegradable ligands of heptagluconate (G7) and gluconate (G6) to test the influence of the Mn2+ :ligand ratio on their fertilizers' capacity to provide Mn to plants. The efficacy of these complexes to correct Mn-deficient soybean was evaluated in hydroponics and calcareous soil conditions and compared with the synthetic chelate EDTA (ethylenediaminetetraacetic acid). RESULTS: This study demonstrated that G7 was a biodegradable alternative to EDTA for supplying Mn, maintaining an adequate nutritional balance compared with G6, which reduced iron (Fe) uptake by the plants. The efficacy of the Mn complexes depended on both the ligand and the Mn:ligand ratio, with the 1:1 and 1:2 molar ratios of Mn2+ :G7 being the most effective complexes in the short term on the basis of their chemical structure and stability. CONCLUSION: The Mn2+ :G7 (1:1 and 1:2) complexes were found to be effective Mn sources for plant nutrition due to their chemical structures providing adequate stability in alkaline solution and their fast-action effect. © 2021 Society of Chemical Industry.

Assessing metal-lignosulfonates as fertilizers using gel filtration chromatography and high-performance size exclusion chromatography.

Lignosulfonates (LSs) are by-products from the paper industry used as biodegradable fertilizers. However, metal-LS ability to provide micronutrients to crops is related to the stability of the complex and the amount of metal complexed. This work evaluated these parameters using ultraviolet-visible (UV-Vis), Fourier-transform infrared (FTIR), and 13C-nuclear magnetic resonance (NMR), along with gel filtration chromatography (GFC) and high-performance size exclusion chromatography (HPSEC), for different spruce, eucalyptus, and pine LSs. GFC and HPSEC pointed out that the amount and type of complexed metal in the LS depends on the molecular weight, pH, and sulphite pulping processes. Both techniques indicated that the low molecular weight LS enriched with phenolic groups has the highest Fe(III) complexing capacity. Also, Fe(III)/LS showed the formation of high molecular weight compounds, whereas Zn(II)/LS and Mn(II)/LS complexes did not form aggregates. Metal-LS fractionation provided considerable information to identify LSs with potential fertilizer capacity and to assess the effectiveness of their complexes.

Effect of Fe:ligand ratios on hydroponic conditions and calcareous soil in Solanum lycopersicum L. and Glycine max L. fertilized with heptagluconate and gluconate.

BACKGROUND: The environmental risk from the application of synthetic chelates has led to the use of biodegradable complexes to correct Fe deficiency in plants. In this article, the Fe oxidation state, the Fe:ligand ratio, and the molecular weight distribution for heptagluconate (G7) and gluconate (G6) are considered as key factors for the efficacy of complexes as fertilizers. Complexes with different Fe:ligand ratios were prepared and analyzed by gel filtration chromatography (GFC). The ability of Fe:ligand ratios to provide Fe to tomato in hydroponics and soybean in calcareous soil was tested and compared with synthetic chelates (Fe3+ :HBED and Fe3+ :EDTA). RESULTS: G7 presented greater capacity to complex both Fe(II) and Fe(III) than G6, but the Fe(II) complexes exhibited poor stability at pH 9 and oxidation in solution. Gel filtration chromatography demonstrated the polynuclear nature of the Fe3+ :G7 at various ratios. The effectiveness of the Fe fertilizers depend on the Fe3+ :ligand ratio and the ligand type, the Fe3+ :G7 (1:1 and 1:2) being the most effective. Fe3+ :G7 (1:1) also presented a better response for the uptake of other micronutrients. CONCLUSION: Fe3+ :G7 molar ratios have been shown to be critical for plant Fe uptake under hydroponic conditions and with calcareous soil. Thus, the Fe3+ :G7 at equimolar ratio and 1:2 molar ratio can be an environmentally friendly alternative to less degradable synthetic chelates to correct Fe chlorosis in strategy I plants. © 2019 Society of Chemical Industry.

Enhancement of the biogas and biofertilizer production from Opuntia heliabravoana Scheinvar.

Waste Opuntia is an abundant source of biomass to produce biogas and biofertilizer in a small and commercial scale. This crop has a high biomass yield, wide adaptation to diverse climatic zones, rapid growth, and low input requirements. This study aimed to evaluate the combined effect of adjusting C/N ratio and an alkaline pretreatment (AP) of waste Opuntia heliabravoana Scheinvar in the production of biogas and biofertilizer in anaerobic reactors. AP bioreactors produced more biogas than the control (C, without the combined effect of AP); besides, in this process, it was not necessary to use additional water due to the high content of water that is present in the tissue of this crop. On the other hand, both biofertilizers (C and AP) had enssential microbial groups that help to enhance plant nutrition as S-reducers, S-oxidizers, amylolytic, cellulolytic bacteria, anaerobic S-mineralizers, cellulolytic fungi, and P-solubilizers. Also, the AP treatment to help to increase 1.5:1 total nitrogen (TN) concentration decreased the pathogenic microorganisms in the biofertilizer compared to the C treatment. For this reason, Opuntia spp. is a good substrate for production of biogas and biofertilizer with essential nutrients for many crops in area with water scarcity.

Effectiveness of rabbit manure biofertilizer in barley crop yield.

The quality of biofertilizers is usually assessed only in terms of the amount of nutrients that they supply to the crops and their lack of viable pathogens and phytotoxicity. The goal of this study was to determine the effectiveness of a liquid biofertilizer obtained from rabbit manure in terms of presence of pathogens, phytotoxicity, and its effect on the grain yield and other agronomic traits of barley (Hordeum vulgare L.). Environmental effects of the biofertilizer were also evaluated by following its influence on selected soil parameters. We applied the biofertilizer at five combinations of doses and timings each and in two application modes (foliar or direct soil application) within a randomized complete block design with three replicates and using a chemical fertilizer as control. The agronomic traits evaluated were plant height, root length, dry weight, and number of leaves and stems at three growth stages: tillering, jointing, and flowering. The effectiveness of the biofertilizer was significantly modified by the mode of application, the growth stage of the crop, and the dose of biofertilizer applied. The results showed that the foliar application of the biofertilizer at the tillering stage produced the highest increase in grain yield (59.7 %, p < 0.10). The use of the biofertilizer caused significant changes in soil, particularly concerning pH, EC, Ca, Zn, Mg, and Mn. It is our view that the production and use of biofertilizers are a reliable alternative to deal with a solid waste problem while food security is increased.