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Eur J Pharmacol ; 954: 175879, 2023 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-37406847

RESUMO

Multiple Sclerosis (MS) is a chronic autoimmune disease of the central nervous system caused by the excessive activation of T cells. Procyanidins are polyphenols that exhibit anti-inflammatory activity. Procyanidin B2 (PCB2) gallate [specifically, PCB2 3,3″-di-O-gallate (PCB2DG)] inhibits cytokine production in T cells by suppressing the acceleration of glycolysis. In this study, we determined the effect of PCB2DG on T cell-mediated autoimmune disease in vivo. We examined the immunosuppressive effects of PCB2DG using an experimental autoimmune encephalomyelitis (EAE) model, which is a classic animal model for MS. Our results indicated that the clinical score for EAE symptoms improved significantly following the oral administration of PCB2DG. This effect was associated with the suppression of T cell-mediated cytokines (e.g., IFN-γ, TNF-α, and IL-17) and infiltrating T cells into the spinal cord, which ameliorated spinal cord injury. In addition, spleen cell culture experiments revealed that the increase of T cell-mediated pro-inflammatory cytokines in EAE mice was significantly decreased following PCB2DG treatment. We further analyzed the glycolytic activity of spleen cells to identify the mechanism of the immunosuppressive effects of PCB2DG. The production of lactate and the expression of glycolytic enzymes and transporters were increased following EAE induction, but not in PCB2DG-treated EAE mice. Collectively, our results indicate that a dietary polyphenol, which has a unique structure, improves the onset of EAE symptoms and inhibits the excessive activation of T cells by influencing glycolysis.


Assuntos
Encefalomielite Autoimune Experimental , Esclerose Múltipla , Proantocianidinas , Animais , Camundongos , Encefalomielite Autoimune Experimental/tratamento farmacológico , Linfócitos T , Proantocianidinas/farmacologia , Proantocianidinas/uso terapêutico , Esclerose Múltipla/tratamento farmacológico , Citocinas/metabolismo , Linfócitos T CD4-Positivos , Glicólise , Administração Oral , Camundongos Endogâmicos C57BL
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