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1.
Cell Stress Chaperones ; 15(6): 1003-11, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20617406

RESUMO

Co-chaperone HOP (also called stress-inducible protein 1) is a co-chaperone that interacts with the cytosolic 70-kDa heat shock protein (HSP70) and 90-kDa heat shock protein (HSP90) families using different tetratricopeptide repeat domains. HOP plays crucial roles in the productive folding of substrate proteins by controlling the chaperone activities of HSP70 and HSP90. Here, we examined the levels of HOP, HSC70 (cognate of HSP70, also called HSP73), and HSP90 in the tumor tissues from colon cancer patients, in comparison with the non-tumor tissues from the same patients. Expression level of HOP was significantly increased in the tumor tissues (68% of patients, n = 19). Levels of HSC70 and HSP90 were also increased in the tumor tissues (95% and 74% of patients, respectively), and the HOP level was highly correlated with those of HSP90 (r = 0.77, p < 0.001) and HSC70 (r = 0.68, p < 0.01). Immunoprecipitation experiments indicated that HOP complexes with HSC70 or HSP90 in the tumor tissues. These data are consistent with increased formation of co-chaperone complexes in colon tumor specimens compared to adjacent normal tissue and could reflect a role for HOP in this process.


Assuntos
Carcinoma/metabolismo , Neoplasias do Colo/metabolismo , Proteínas de Choque Térmico HSP70/metabolismo , Proteínas de Choque Térmico HSP90/metabolismo , Proteínas de Choque Térmico/metabolismo , Benzoquinonas/farmacologia , Carcinoma/patologia , Neoplasias do Colo/patologia , Humanos , Imunoprecipitação , Lactamas Macrocíclicas/farmacologia , Ligação Proteica , Dobramento de Proteína
2.
Chemistry ; 12(20): 5328-33, 2006 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-16622884

RESUMO

Thermal Z to E isomerization reactions of azobenzene and 4-dimethylamino-4'-nitroazobenzene were examined in three ionic liquids of general formula 1-R-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (R = butyl, pentyl, and hexyl). The first-order rate constants and activation energies for the reactions of azobenzene measured in these ionic liquids were consistent with those measured in ordinary organic solvents, which indicated that the slow isomerization through the inversion mechanism with a nonpolar transition state was little influenced by the solvent properties, such as the viscosity and dielectric constant, of ionic liquids. On the other hand, the rate constants and the corresponding frequency factors of the Arrhenius plot were significantly reduced for the isomerization of 4-dimethylamino-4'-nitroazobenzene in ionic liquids compared with those for the isomerization in ordinary organic molecular solvents with similar dielectric properties. Although these ionic liquids are viscous, the apparent viscosity dependence of the rate constant could not be explained either by the Kramers-Grote-Hynes model or by the Agmon-Hopfield model for solution reactions. It is proposed that the positive and the negative charge centers of a highly polar rotational transition state are stabilized by the surrounding anions and cations, respectively, and that the ions must be rearranged so as to form highly ordered solvation shells around the charge centers of the reactant in the transition state. This requirement for the orderly solvation in the transition state results in unusually small frequency factors of 10(4)-10(7) s(-1).

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