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1.
Plant Physiol ; 151(4): 1823-30, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19854856

RESUMO

Widely conserved among eukaryotes, the microtubule-associated protein 215 (MAP215) family enhances microtubule dynamic instability. The family member studied most extensively, Xenopus laevis XMAP215, has been reported to enhance both assembly and disassembly parameters, although the mechanism whereby one protein can exert these apparently contradictory effects has not been clarified. Here, we analyze the activity of a plant MAP215 homolog, tobacco (Nicotiana tabacum) MAP200 on microtubule behavior in vitro. We show that, like XMAP215, MAP200 promotes both assembly and disassembly parameters, including microtubule growth rate and catastrophe frequency. When MAP200 is added to tubulin and taxol, strikingly long-coiled structures form. When GDP partially replaces GTP, the increase of catastrophe frequency by MAP200 is strongly diminished, even though this replacement stimulates catastrophe in the absence of MAP200. This implies that MAP200 induces catastrophes by a specific, GTP-requiring pathway. We hypothesize that, in the presence of MAP200, a catastrophe-prone microtubule lattice forms occasionally when elongated but nonadjacent protofilaments make lateral contacts.


Assuntos
Guanosina Trifosfato/metabolismo , Proteínas Associadas aos Microtúbulos/química , Microtúbulos/metabolismo , Nicotiana/efeitos dos fármacos , Nicotiana/metabolismo , Proteínas de Plantas/farmacologia , Homologia de Sequência de Aminoácidos , Proteínas de Xenopus/química , Animais , Guanosina Difosfato/farmacologia , Guanosina Trifosfato/farmacologia , Microtúbulos/efeitos dos fármacos , Modelos Biológicos , Paclitaxel/farmacologia , Proteínas de Plantas/ultraestrutura , Tubulina (Proteína)/metabolismo , Tubulina (Proteína)/ultraestrutura , Xenopus
2.
J Mol Biol ; 391(5): 849-57, 2009 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-19580814

RESUMO

The process of microtubule elongation is thought to consist of two stages-formation of a tubulin sheet structure and its closure into a tube. However, real-time observation of this process has been difficult. Here, by utilizing phospho-tau binding protein Gas7 (growth-arrest-specific protein 7), we visualized the polymer transformation process by dark-field microscopy. Upon elongation, thin and flexible structures, often similar to a curved hook, appeared at the end of microtubules. Electron microscopic observations supported the idea that these flexible structures are tubulin sheets. They maintained their length until they gradually became thick and rigid beginning in the central portion, resulting in straight microtubules. In the absence of Gas7, the sheet-like structure was rarely observed; moreover, when observed, it was fragile and engaged in typical dynamic instability. With Gas7, no catastrophe was observed. These results suggest that Gas7 enhances microtubule polymerization by stabilizing sheet intermediates and is a useful tool for analyzing microtubule transformation.


Assuntos
Microtúbulos , Proteínas do Tecido Nervoso/metabolismo , Tubulina (Proteína) , Animais , Bovinos , Camundongos , Microscopia/métodos , Microtúbulos/metabolismo , Microtúbulos/ultraestrutura , Proteínas do Tecido Nervoso/genética , Suínos , Resistência à Tração , Tubulina (Proteína)/metabolismo , Tubulina (Proteína)/ultraestrutura
3.
Blood ; 113(2): 458-61, 2009 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-18849486

RESUMO

Congenital macrothrombocytopenia is a genetically heterogeneous group of rare disorders. We identified the first TUBB1 mutation, R318W, in a patient with congenital macrothrombocytopenia. The patient was heterozygous for Q43P, but this single-nucleotide polymorphism (SNP) did not relate to macrothrombocytopenia. Although no abnormal platelet beta1-tubulin localization/marginal band organization was observed, the level of beta1-tubulin was decreased by approximately 50% compared with healthy controls. Large and irregular bleb protrusions observed in megakaryocytes derived from the patient's peripheral blood CD34(+) cells suggested impaired megakaryocyte fragmentation and release of large platelets. In vitro transfection experiments in Chinese hamster ovary (CHO) cells demonstrated no incorporation of mutant beta1-tubulin into microtubules, but the formation of punctuated insoluble aggregates. These results suggested that mutant protein is prone to aggregation but is unstable within megakaryocytes/platelets. Alternatively, mutant beta1-tubulin may not be transported from the megakaryocytes into platelets. W318 beta1-tubulin may interfere with normal platelet production, resulting in macrothrombocytopenia.


Assuntos
Doenças Genéticas Inatas/genética , Doenças Genéticas Inatas/metabolismo , Microtúbulos/genética , Microtúbulos/metabolismo , Mutação de Sentido Incorreto , Púrpura Trombocitopênica Idiopática/genética , Púrpura Trombocitopênica Idiopática/metabolismo , Tubulina (Proteína)/genética , Tubulina (Proteína)/metabolismo , Substituição de Aminoácidos , Animais , Plaquetas/metabolismo , Células CHO , Criança , Cricetinae , Cricetulus , Heterozigoto , Humanos , Masculino , Megacariócitos/metabolismo , Polimorfismo de Nucleotídeo Único
4.
J Cell Sci ; 121(Pt 14): 2372-81, 2008 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-18577573

RESUMO

SPIRAL2 (SPR2) of Arabidopsis thaliana is a microtubule-associated protein containing multiple HEAT repeats that are found only in the plant lineage. We show that SPR2 and SP2L, their closest Arabidopsis homolog, are expressed in various tissues with partially overlapping patterns, and spr2-sp2l double mutants exhibit enhanced right-handed helical growth. Fusion to green fluorescent protein (GFP) expressed under the control of the native regulatory elements showed that both SPR2 and SP2L were localized to cortical microtubules, mainly in particles of various sizes. Along the microtubule, the GFP-fused forms also distributed partly at the plus ends. In the spr2-mutant background, cortical microtubules were less dynamic, and the pause state - in which microtubules undergo neither growth nor shrinkage - increased at the plus ends. The continuous plus-end tracking of GFP-EB1 was occasionally interrupted in the mutant cells. Recombinant SPR2 protein promoted microtubule polymerization, and bound to microtubules with an N-terminal segment that contained two HEAT repeats as well as to those with a C-terminal region. In vitro analyses of microtubule dynamics revealed that SPR2 and SP2L suppressed the pause state at microtubule ends, thereby leading to enhanced microtubule growth. We propose that the SPR2-family proteins act on the pause state to facilitate a transition to microtubule growth.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/crescimento & desenvolvimento , Proteínas Associadas aos Microtúbulos/metabolismo , Microtúbulos/metabolismo , Arabidopsis/citologia , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Regulação da Expressão Gênica de Plantas , Proteínas de Fluorescência Verde/metabolismo , Hipocótilo/metabolismo , Proteínas Associadas aos Microtúbulos/genética , Ligação Proteica , Transporte Proteico , Proteínas Recombinantes de Fusão/metabolismo , Plântula/metabolismo
5.
J Neurosci ; 27(48): 13098-107, 2007 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-18045904

RESUMO

Neurons are highly polarized cells that possess two morphologically and functionally different types of protrusions, axons and dendrites, that function in the transmission and reception of neural signals, respectively. A great deal of attention has been paid to the specification and guidance of axons, but the mechanism of dendrite development remains mostly unknown. We report here that a polarity-regulating kinase, partitioning-defective 1 (Par1b)/microtubule affinity-regulating kinase 2 (MARK2), specifically regulates development of dendrites in hippocampal neurons. Ectopic expression of Par1b/MARK2 shortens the length and decreases branching of dendrites without significant effects on axons. Knockdown of endogenous Par1b/MARK2 by RNA interference stimulates dendrite development. Wnt stimulation and Dishevelled expression, both of which are known to induce dendrite development, induced recruitment of Par1b/MARK2 to the membrane fraction. Expression of a Par1b/MARK2 mutant, that contains a myristoylation signal and accumulates exclusively in membranes, does not affect dendrite development. In addition, Par1b/MARK2 efficiently phosphorylated MAP2, which is localized mainly in dendrites. These results indicate that Par1b/MARK2 negatively regulates dendrite development through phosphorylation of MAP2.


Assuntos
Dendritos/fisiologia , Hipocampo/citologia , Neurônios/citologia , Proteínas Serina-Treonina Quinases/fisiologia , Animais , Células Cultivadas , Chlorocebus aethiops , Embrião de Mamíferos , Proteínas de Fluorescência Verde/metabolismo , Humanos , Camundongos , Proteínas Associadas aos Microtúbulos/metabolismo , Mutação/fisiologia , Nocodazol/farmacologia , RNA de Cadeia Dupla/genética , RNA de Cadeia Dupla/farmacologia , Ratos , Frações Subcelulares/metabolismo , Transfecção/métodos , Tubulina (Proteína)/metabolismo , Moduladores de Tubulina/farmacologia , Proteínas Wnt/farmacologia , Proteína Wnt3
6.
Cell Signal ; 18(12): 2182-92, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16820281

RESUMO

Regulator of G-protein signaling (RGS) proteins interact with alpha subunits of heterotrimeric G-proteins via the RGS domain and attenuate their activity by accelerating GTPase activity. RGS2, a member of the RGS family, regulates synaptic development via hereto unknown mechanism. In this study, we found that RGS2 directly interacted with tubulin via a short region at the N-terminus: amino acids 41-60. RGS2 enhanced microtubule polymerization in vitro, and the tubulin binding region was necessary and sufficient for this activity. In Vero cells, polymerization of microtubule was stimulated when peptides containing the tubulin binding region were microinjected. Immunocytochemical analysis showed that endogenous RGS2 was localized at the termini of neurites in differentiated PC12 cells. Over-expression of RGS2 enhanced the nerve growth factor-induced neurite outgrowth in PC12 cells, while specific knock-down of endogenous RGS2 suppressed the neurite outgrowth. These findings demonstrate that RGS2 contributes to the neuronal cell differentiation via regulation of microtubule dynamics.


Assuntos
Microtúbulos/metabolismo , Neuritos/fisiologia , Proteínas RGS/metabolismo , Sequência de Aminoácidos , Animais , Sítios de Ligação/genética , Encéfalo/metabolismo , Células COS , Diferenciação Celular/genética , Diferenciação Celular/fisiologia , Chlorocebus aethiops , Glutationa Transferase/genética , Glutationa Transferase/metabolismo , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Microscopia Confocal , Microscopia Eletrônica , Microtúbulos/ultraestrutura , Mutação/genética , Neuritos/metabolismo , Neuritos/ultraestrutura , Células PC12 , Ligação Proteica , Proteínas RGS/química , Proteínas RGS/genética , Interferência de RNA/fisiologia , RNA Interferente Pequeno/genética , Ratos , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Transfecção/métodos , Tubulina (Proteína)/genética , Tubulina (Proteína)/metabolismo , Células Vero
7.
Plant Cell Physiol ; 47(7): 1004-9, 2006 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16699178

RESUMO

The effects of butanol on microtubules (MTs) were examined by immunofluorescence microscopy. Fragmentation of cortical MTs was induced by n-butanol, but not by s- and t-butanols, in cultured tobacco BY-2 cells. Taxol prevented n-butanol-induced MT fragmentation. Fragmented cortical MTs were still attached to the inner face of the plasma membrane when n-butanol-treated protoplasts were ruptured on the slide glass. Moreover, MTs were depolymerized in the presence of n-butanol in vitro. Therefore, n-butanol is not only an activator of phospholipase D but also an effective MT-depolymerizing agent.


Assuntos
1-Butanol/farmacologia , Microtúbulos/efeitos dos fármacos , Nicotiana/efeitos dos fármacos , Células Cultivadas , Ativação Enzimática/efeitos dos fármacos , Microscopia de Fluorescência , Microtúbulos/ultraestrutura , Fosfolipase D/efeitos dos fármacos , Fosfolipase D/fisiologia , Nicotiana/ultraestrutura
9.
Genes Dev ; 20(8): 1004-14, 2006 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-16598040

RESUMO

The tobacco mitogen-activated protein kinase (MAPK) cascade, which includes MAPK NRK1/NTF6, positively regulates expansion of the cytokinetic machinery known as the phragmoplast, which is followed by the synthesis of cell plates for completion of cell division. However, molecular events lying between the MAPK and phragmoplast expansion were not known. Here, we show that NRK1/NTF6 phosphorylates the threonine residue at position 579 in NtMAP65-1a, a microtubule-associated (MT-associated) protein. Levels of phosphorylated NtMAP65-1 increase during late M phase of the cell cycle, when NRK1/NTF6 is activated. Phosphorylated NtMAP65-1 is concentrated at the equator of phragmoplast, as is NRK1/NTF6. Overexpression of mutant forms of NtMAP65-1a that cannot be phosphorylated by NRK1 delays progression of the M phase and phragmoplast expansion, also rendering phragmoplast structures resistant to an MT-depolymerizing drug. Phosphorylation of NtMAP65-1 by NRK1/NTF6 down-regulates its MT-bundling activity in vitro. These results suggest that phosphorylation of NtMAP65-1 by NRK1/NTF6 also reduces its MT-bundling activity in vivo, which enhances destabilization and turnover of MTs at the phragmoplast equator, perhaps facilitating phragmoplast expansion.


Assuntos
Ciclo Celular , Regulação para Baixo , Proteínas Associadas aos Microtúbulos/metabolismo , Microtúbulos/metabolismo , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Nicotiana/citologia , Microscopia de Fluorescência , Proteínas Associadas aos Microtúbulos/química , Proteínas Associadas aos Microtúbulos/genética , Mutagênese Sítio-Dirigida , Fosforilação , Plasmídeos , Treonina/metabolismo
10.
Cell Struct Funct ; 29(5-6): 147-57, 2005 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15840946

RESUMO

MAP4, a ubiquitous heat-stable MAP, is composed of an asymmetric structure common to the heat-stable MAPs, consisting of an N-terminal projection (PJ) domain and a C-terminal microtubule (MT)-binding (MTB) domain. Although the MTB domain has been intensively studied, the role of the PJ domain, which protrudes from MT-wall and does not bind to MTs, remains unclear. We investigated the roles of the PJ domain on the dynamic instability of MTs by dark-field microscopy using various PJ domain deletion constructs of human MAP4 (PJ1, PJ2, Na-MTB and KDM-MTB). There was no obvious difference in the dynamic instability between the wtMAP4 and any fragments at 0.1 microM, the minimum concentration required to stabilize MTs. The individual MTs stochastically altered between polymerization and depolymerization phases with similar profiles of length change as had been observed in the presence of MAP2 or tau. We also examined the effects at the increased concentrations of 0.7 microM, and found that in some cases the dynamic instability was almost entirely attenuated. The length of both the polymerization and depolymerization phases decreased and "pause-phases" were occasionally observed, especially in the case of PJ1, PJ2 or Na-MTB. No obvious change was observed in the increased concentration of wtMAP4 and KDM-MTB. Additionally, the profiles of MT length change were quite different in 0.7 microM PJ2. Relatively rapid and long depolymerization phases were sometimes observed among quite slow length changes. Perhaps, this unusual profile could be due to the uneven distribution of PJ2 along the MT lattice. These results indicate that the PJ domain of MAP4 participates in the regulation of the dynamic instability.


Assuntos
Proteínas dos Microtúbulos/química , Proteínas dos Microtúbulos/metabolismo , Proteínas Associadas aos Microtúbulos/química , Proteínas Associadas aos Microtúbulos/metabolismo , Microtúbulos/metabolismo , Sequência de Aminoácidos , Humanos , Proteínas dos Microtúbulos/análise , Proteínas dos Microtúbulos/genética , Proteínas Associadas aos Microtúbulos/análise , Microtúbulos/química , Mutação , Estrutura Terciária de Proteína
11.
Plant Cell Physiol ; 45(9): 1233-42, 2004 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-15509846

RESUMO

A microtubule-associated protein composed of a 200 kDa polypeptide (MAP200) was isolated from tobacco-cultured BY-2 cells. Analysis of the partial amino acid sequence showed that MAP200 was identical to TMBP200, the tobacco MOR1/XMAP215 homolog. Although several homolog proteins in animal and yeast cells have been reported to promote MT dynamics in vitro, no such function has been reported for plant homologs. Turbidity measurements of tubulin solution suggested that MAP200 promoted tubulin polymerization, and analysis by dark-field microscopy revealed that this MAP increased both the number and length of microtubules (MTs). Electron microscopy and experiments using a chemical crosslinker demonstrated that MAP200 forms a complex with tubulin. Throughout the cell cycle, some MAP200 colocalized with MT structures, including cortical MTs, the preprophase band, spindle and phragmoplast, while some MAP200 was localized in areas lacking MTs. Based on our biochemical and immunofluorescence findings, the function of MAP200 in MT polymerization is discussed.


Assuntos
Proteínas Associadas aos Microtúbulos/metabolismo , Nicotiana/citologia , Biopolímeros , Linhagem Celular , Dimerização , Microscopia de Fluorescência , Proteínas Associadas aos Microtúbulos/química , Proteínas Associadas aos Microtúbulos/isolamento & purificação , Peso Molecular , Ligação Proteica
12.
J Biol Chem ; 279(22): 23014-21, 2004 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-15039434

RESUMO

RB3 is a neuron-specific homologue of the SCG10/stathmin family proteins, possessing a unique N-terminal membrane-associated domain and the stathmin-like domain at the C terminus, which promotes microtubule (MT) catastrophe and/or tubulin sequestering. We examined herein the contribution of the N-terminal subdomain of RB3 to the regulation of MT dynamics. To begin with, we determined the effects of full-length (RB3-f) and short truncated (RB3-s) forms of RB3 on the polymerization of MT in vitro. RB3-s had a deletion of amino acids 1-75 from the N terminus, leaving the so-called stathmin-like domain, consisting of residues 76-217. Although both RB3-f and RB3-s exhibited MT-depolymerizing activity, RB3-f was less effective. The binding affinity for tubulin was also lower in RB3-f. Direct observation of the dynamics of individual MTs using dark field microscopy revealed that RB3-s slowed MT elongation velocity, increased catastrophes, and reduced rescues. This effect is almost identical to that by stathmin/oncoprotein 18. On the other hand, the MT elongation rate increased at lower concentrations of RB3-f. In addition, RB3-f, indicated higher rescue frequency than control as well as the catastrophe in a dose-dependent manner. The functionality of RB3-f indicated that full-length RB3 has not only stathmin-like MT destabilizing activity but also MT-associated protein-like MT stabilizing activity. Possibly, the balance of these activities is altered in a concentration-dependent manner in vitro. This interesting regulatory role of the unique N-terminal domain of RB3 in MT dynamics would contribute to the physiological regulation of neuronal morphogenesis.


Assuntos
Microtúbulos/metabolismo , Fatores de Crescimento Neural/metabolismo , Animais , Sítios de Ligação , Diferenciação Celular , Microtúbulos/ultraestrutura , Neurônios/fisiologia , Ligação Proteica , Estrutura Terciária de Proteína , Ratos , Relação Estrutura-Atividade , Tubulina (Proteína)/metabolismo
13.
Biol Sci Space ; 18(3): 116-7, 2004 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-15858348

RESUMO

Individual microtubules (MTs) repeat alternating phases of polymerization and depolymerization, a process known as "dynamic instability." The dynamic instability is regulated by various protein factors according to the requirement of cellular conditions. Heat-stable MAPs regulate the dynamic instability by increasing the rescue frequency. To explore the influence of MAP2, a heat-stable MAPs abundant in neuron, on in vitro MT dynamics, the distribution of MAP2 on individual MTs was correlated with the dynamic phase changes of the same MTs by optical microscopy. MAP2 distributed inhomogeneously along the length of MTs by forming high-density regions, clusters. Stops of depolymerization were always found to occur only at the cluster sites. Every cluster did not stop depolymerization, but depolymerization did always stop at a cluster site. We suggest that mode of distribution along MT is an important factor of the function of heat-stable MAPs.


Assuntos
Proteínas Associadas aos Microtúbulos/fisiologia , Microtúbulos/fisiologia , Guanosina Difosfato/fisiologia , Guanosina Trifosfato/fisiologia , Ligação Proteica , Tubulina (Proteína)/fisiologia
14.
Biochem Biophys Res Commun ; 305(1): 72-8, 2003 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-12732198

RESUMO

To see a molecular basis of the difference in the microtubule binding between MAP2 and MAP4, we compared the binding of them onto microtubule and Zinc-sheet in the presence of various concentrations of NaCl. The Zinc-sheet is the lateral association of protofilaments arranged in an antiparallel fashion with alternatively exposed opposite surfaces, so that binding requiring adjacent protofilaments is restricted. While the salt-dependence of the MAP2 desorption was not altered between these tubulin polymers, MAP4 dissociated from Zinc-sheet at lower concentrations of NaCl than from microtubule. These results suggest that single protofilament is sufficient for microtubule binding of MAP2 as observed by Al-Bassam et al. [J. Cell Biol. 157 (2002) 1187], but MAP4 appeared to interact with adjacent protofilaments during microtubule-binding. Weakened binding on Zinc-sheets was also observed in the projection domain-deletion mutants of MAP4, so that the difference in the protofilament-dependence would lie in the relatively conserved microtubule-binding domain.


Assuntos
Proteínas Associadas aos Microtúbulos/metabolismo , Microtúbulos/metabolismo , Animais , Sítios de Ligação , Proteínas Associadas aos Microtúbulos/química , Proteínas Associadas aos Microtúbulos/genética , Microtúbulos/química , Prolina/análise , Ligação Proteica/efeitos dos fármacos , Estrutura Terciária de Proteína , Deleção de Sequência , Cloreto de Sódio/farmacologia
15.
J Biol Chem ; 278(20): 18538-43, 2003 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-12626509

RESUMO

Septins are a family of conserved proteins implicated in a variety of cellular functions such as cytokinesis and vesicle trafficking, but their properties and modes of action are largely unknown. Here we now report findings of immunocytochemical and biochemical characterization of a mammalian septin, MSF-A. Using an antibody specific for MSF subfamily proteins, MSF-A was found to be expressed predominantly in mammary human mammary epithelial cells (HMEC). MSF-A was associated with microtubules in interphase HMEC cells as it localized with the mitotic spindle and the bundle of microtubule at midzone during mitosis. Biochemical analysis revealed direct binding of MSF-A with polymerized tubulin through its central region containing guanine nucleotide-interactive motifs. GTPase activity, however, was not required for the association. Conditions that disrupt the microtubule network also disrupted the MSF-A-containing filament structure, resulting in a punctate cytoplasmic pattern. Depletion of MSF-A using small interfering RNAs caused incomplete cell division and resulted in the accumulation of binucleated cells. Unlike Nedd5, an MSF mutant deficient in GTPase activity forms filament indistinguishable from that of the wild type in COS cells. These results strongly suggest that septin filaments may interact not only with actin filaments but also with microtubule networks and that GTPase activity of MSF-A is not indispensable to incorporation of MSF-A into septin filaments.


Assuntos
Mama/metabolismo , Proteínas do Citoesqueleto/química , Células Epiteliais/metabolismo , Proteínas de Ligação ao GTP/química , Motivos de Aminoácidos , Animais , Células COS , Linhagem Celular , Células Cultivadas , Citocalasina B/farmacologia , Citoplasma/metabolismo , Proteínas do Citoesqueleto/metabolismo , Demecolcina/farmacologia , GTP Fosfo-Hidrolases/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Células HeLa , Humanos , Microscopia de Fluorescência , Microtúbulos/metabolismo , Mitose , Mutação , Plasmídeos/metabolismo , Ligação Proteica , Interferência de RNA , Proteínas Recombinantes/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transfecção , Tubulina (Proteína)/química
16.
Nat Cell Biol ; 4(8): 583-91, 2002 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-12134159

RESUMO

Regulated increase in the formation of microtubule arrays is thought to be important for axonal growth. Collapsin response mediator protein-2 (CRMP-2) is a mammalian homologue of UNC-33, mutations in which result in abnormal axon termination. We recently demonstrated that CRMP-2 is critical for axonal differentiation. Here, we identify two activities of CRMP-2: tubulin-heterodimer binding and the promotion of microtubule assembly. CRMP-2 bound tubulin dimers with higher affinity than it bound microtubules. Association of CRMP-2 with microtubules was enhanced by tubulin polymerization in the presence of CRMP-2. The binding property of CRMP-2 with tubulin was apparently distinct from that of Tau, which preferentially bound microtubules. In neurons, overexpression of CRMP-2 promoted axonal growth and branching. A mutant of CRMP-2, lacking the region responsible for microtubule assembly, inhibited axonal growth and branching in a dominant-negative manner. Taken together, our results suggest that CRMP-2 regulates axonal growth and branching as a partner of the tubulin heterodimer, in a different fashion from traditional MAPs.


Assuntos
Microtúbulos/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Tubulina (Proteína)/metabolismo , Animais , Axônios/metabolismo , Axônios/ultraestrutura , Linhagem Celular , Chlorocebus aethiops , Dimerização , Fibroblastos/metabolismo , Proteínas de Fluorescência Verde , Hipocampo/metabolismo , Técnicas In Vitro , Peptídeos e Proteínas de Sinalização Intercelular , Cinética , Proteínas Luminescentes/metabolismo , Mutação , Proteínas do Tecido Nervoso/química , Proteínas do Tecido Nervoso/genética , Neurônios/metabolismo , Ligação Proteica , Ratos , Proteínas Recombinantes de Fusão/metabolismo , Tubulina (Proteína)/química , Células Vero
17.
J Mol Biol ; 320(1): 97-106, 2002 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-12079337

RESUMO

Microtubule-associated protein 4 (MAP4), a major MAP expressed in proliferating non-neuronal cells, consists of an N-terminal projection (PJ) domain and a C-terminal microtubule-binding (MTB) domain. The PJ domain of MAP4 is divided into three regions; the N-terminal acidic region (the Na-region), the multiple KDM-repeated sequence region (the KDM-region), and the b-region followed by the MTB domain. To investigate roles of the PJ domain, we prepared three truncated forms of human MAP4 with different PJ domain lengths; PJ1, PJ2 and MTB with deletion of about one-third, two-third and all of the PJ domain, respectively, and examined their effects on bundle formation of microtubules (MTs). MTs polymerized by full length MAP4 were singly distributed as observed by both negative staining electron microscopy and dark field microscopy. MTs with PJ1 were also separated in solution but became pairs when pelleted by centrifugation. PJ2 formed planar two-dimensional bundles consisting of several MTs (the 2D-bundle). MTB induced large bundles of many MTs, tightly packed without space in between (termed the 3D-bundle). To study how the PJ domain decreases the bundle-forming activity of the MTB domain of MAP4, we made three additional deletion-mutants of MAP4, called Na-MTB, KDM-MTB and Na-PJ2. Na-MTB and KDM-MTB, in which the KDM/b-region and both of Na- and b-regions were deleted respectively, were prepared by fusing the Na-region or KDM-region to MTB. Both of Na-MTB and KDM-MTB suppressed the 3D-bundle formation as effectively as PJ2. MTs polymerized with Na-PJ2, the KDM-deletion mutant made by adding the Na-region to PJ2, were singular and did not become bundles. These results indicated that the PJ domain kept individual MTs separated by suppressing the bundle-forming ability of the MTB domain. The suppressive activity of the PJ domain was correlated with the length, but not the amino acid sequence, of the PJ.


Assuntos
Proteínas Associadas aos Microtúbulos/química , Proteínas Associadas aos Microtúbulos/metabolismo , Microtúbulos/metabolismo , Fragmentos de Peptídeos/metabolismo , Animais , Escherichia coli/fisiologia , Humanos , Proteínas Associadas aos Microtúbulos/genética , Microtúbulos/ultraestrutura , Fragmentos de Peptídeos/genética , Ligação Proteica , Estrutura Terciária de Proteína
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