[Ultrastructural organization of carcinoma Lewis (3LL) cells during cisplatin resistance formation].

The electron-microscopic analysis of the morphological status of 3LL (Lewis) carcinoma tumour cells in the process of cisplatin resistant phenotype formation has been performed. It was shown that selection of tumour cells forming cell clones characterized by more complicated nuclear and cytoplasm organization took place. The tumour cells had the diffused nuclear chromatin; nuclear envelope had the numerous pores with expanded diaphragms. The prominent nucleoli consisted of the active centres surrounded by considerable areas of the condensed nucleolar chromatin. Cell cytoplasm contained the well-developed Goldgi complex and the numerous well-structured myelinoid formations in the form of dense-wrapped concentric membrane structures. The obtained data can morphologically confirm the hypothesis of Gately D.P. and Howel S.B., 1993, thain the process of resistant phenotype formation the tumour cells can create the cellular mechanisms to remove the drug from the cell and to correct the damages of the cellular nucleus and cytoplasm.

[Use of WOBE-MUGOS E for prevention and correction of experimental doxorubicin side-effects]. / Ispol'zovanie VOBE-MUGOS E dlia profilaktiki i korrektsii pobochnykh reaktsii doksorubitsina gidrokhlorida v éksperimente.

Examined in treating rats with Guerin's carcinoma with doxorubicin was the possibility that the polyenzymic drug preparation wobe-mugos E had hepatoprotective, cardioprotective and myeloprotective effects. In intramuscular administration wobe-mugos E has not been found to stimulate the tumour growth, it exhibited a manifest hepatoprotective and myeloprotective actions with respect to adverse reactions of doxorubicin but failed to diminish cardiotoxicity of the latter. The protective effect of the above polyenzymic drug was of a dose-dependent character.

[The enterosorbents belosorb and panzysorb in the treatment of acute experimental pancreatitis in rats]. / Enterosorbenty belosorb i panzisorb u likuvanni eksperymental'noho hostroho pankreatytu u shchuriv.

Enterosorption with highly dispersed fibrous carbon adsorbent Belosorb or enzyme-containing fibrous carbon adsorbent Panzysorb was applied for the treatment of experimental acute pancreatitis (EAP) in the rats. Panzysorb was obtained by immobilization of digestive hydrolases (proteases and amylase) on Belosorb as matrix. It was shown that oral administration of Panzysorb (100 mg/kg body weight) significantly decreased amylolytic and lipolytic activity of blood serum by the third hour after EAP induction. The course of enterosorption (ten days) with the Panzysorb led to the appearance of regeneration focuses in injured organ.

[Aggressive fibromatosis of the small pelvis]. / Agressivnyi fibromatoz polosti malogo taza.

Experience in the treatment of 25 patients with aggressive fibromatosis of the true pelvis is discussed. To establish a precise diagnosis obligatory and special methods of examination were applied. Secondary changes in the adjacent organs and structures were found in 24 patients. Radical operations were conducted on 19 (76%) patients, in 13 of them the interventions were combined. The mean duration of the operations was 5.3 hours. Complications developed in 17 out of 19 patients. Hemorrhage was the most frequent (in 12 out of 19 patients) and menacing complication. The blood loss was 3036.8 ml on the average. Recurrences were registered in 8 out of 19 (42.1%) patients.

[Current theories on the origin of Reed-Sternberg cells in lymphogranulomatosis]. / Sovremennye predstavleniia o proiskhozhdenii kletok Berezovskogo- Shternberga pri limfogranulematoze.

The literature data and the results of the authors' own investigations on the enzymatocytochemical signs, surface membrane receptors and antigens of Hodgkin's and Reed-Sternberg cells have been presented. Possible genesis of giant multinuclear cells from activated T- or B-lymphocytes, histiocytes,+-macrophages or interdigitirring reticular cells in lymphogranulomatosis have been discussed.

[Monoclonal antibodies of the IPO series in studying and diagnosing malignant lymphoproliferative diseases]. / Monoklonal'nye antitela serii IPO v izuchenii i diagnostike zlokachestvennykh limfoproliferativnykh zabolevanii.

The authors have developed a panel of monoclonal antibodies (MCA) of IPO series (Institute of Problems of Oncology, Kiev). RPMI-1788 cell line, Daudi, as well as splenocytes of a patient with hairy-cell leukemia were used for immunization of mice. It has been shown that IPO-3, IPO-10 and IPO-24 MCA are directed against differentiation antigens of human B-lymphocytes. IPO-4 MCA reveal the antigen of activated T- and B-cells. IPO-5 and IPO-20 MCA recognize HLA-ABC, while IPO-37 a common leucocytic antigen. IPO-38 MCA are directed against the nuclear antigen. The possibilities of using IPO MCA in hematology have been discussed.

[Monoclonal antibodies IPO-4 recognizing antigen-activated human T and B lymphocytes]. / Monoklonal'nye antitela IPO-4, raspoznaiushchie antigen-aktirovannye T- i B-limfotsity cheloveka.

Spleen cells from BALB/c mice previously immunized with B-lymphoblastoid cell line RPMI-1788 were fused with P3-X-63-Ag8.653 myeloma cells. Monoclonal antibodies (Ab) IPO-4 were screened on 18 cell lines by the indirect immuno-fluorescence method. Cryostat sections of tissues were stained according to the PAP technique. The Mab IPO-4 were tested for reactivity with blood cells of 17 healthy persons and 102 patients with chronic lymphocytic leukemia, acute lymphoblastic and myeloblastic leukemias, hairy cell leukemia, multiple myeloma, non-Hodgkin's lymphomas and Hodgkin's disease and mitogen stimulated lymphocytes. MAb IPO-4 were found to be directed against antigen expressed on activated T and B cells.

[Morphocytochemical and electron microscopic research on murine hybridoma cells producing and not producing monoclonal antibodies]. / Morfotsitokhimicheskoe i élektronno-mikroskopicheskoe issledovanie kletok myshinykh gibridom, produtsiruiushchikh i neprodutsiruiushchikh monoklonal'nye antitela.

Cytochemical and ultrastructural features of mouse hybridomas and also of the parental cells--myeloma P3-X63-Ag8.653 and spleen cells of the Balb/c mice immunized with cell line RPMI-1788 have been studied. Differences in cytomorphological signs and activity of acid phosphatase, acid nonspecific esterase, nonspecific-alpha-naphthyl acetate esterase were shown in hybrid cell lines secreting and not secreting monoclonal antibodies.

[Immunological and electron microscope characteristics of peripheral blood lymphocytes in myeloma disease]. / Immunologicheskaia i élektronno-mikroskopicheskaia kharakteristika limfotsitov perifericheskoi krovi pri mielomnoi bolezni.

B-lymphocytes of peripheral blood were examined with cytochemical, immunological and electron-microscopic methods in 18 patients with myeloma. The results made it possible to divide all patients into 3 groups distinguishing by cytomorphology of peripheral blood lymphocytes, expression of surface and cytoplasmic immunoglobulins and determination on the surface membrane of receptors to Fc-fragments of IgG and IgM and C3-component of the complement. Data revealed clonal character of B-lymphocytes.

[Evaluation of the distribution of lectin receptors on the surface of tumor and transformed cells using methods of variation statistics]. / Otsenka raspredeleniia lektinovykh retseptorov na poverkhnosti opukholevykh i transformirovannykh kletok s pomoshch'iu metodov variatsionnoi statistiki.

Statistical procedures were used to estimate lectin receptor distribution on the surface of ascite lymphoma cells, neuroblastoma C-1300 cells and of transformed human T- and B-derived lymphoid cell lines. Relationships between the arithmetic means and mean square variances for sample populations from each cell and ferritin- or colloidal gold-lectin combination were used to define four types of topographical distributions: uniform-ordered, uniform-random, random and clustered.

[Ultrastructural organization of the blood lymphoid cells in hairy cell leukemia]. / Ul'trastrukturnaia organizatsiia limfoidnykh kletok krovi pri volosatokletochnom leikoze.

The cytochemical, immunological, and ultrastructural peculiarities of the hairy-cell leukemia cells are studied. The hairy cells can be identified by characteristic of ultrastructure of organellae: numerous thin processes on the plasma membrane, large cytoplasmic area, well developed granular endoplasmic reticulum, numerous vesicules and electron dense granules, a well differentiated lobulated nucleus with a large nucleolus in which the granular component predominates.

[Cell surface receptors in lymphoid lines studied using lectins]. / Issledovanie retseptorov na poverkhnosti kletok limfoidnykh linii s pomoshch'iu lektinov.

The nature and distribution of some carbohydrate receptors on the cell membrane of transformed human B- and T-derived lymphocyte cell lines (RPMI-1788, Daudi, Jurkat, Molt-4) are studied by Ricinus communis agglutinin, soybean agglutinin, Helix pomatia lectin, peanut agglutinin and wheat germ agglutinin conjugated with ferritin and colloidal gold. The quality of receptors and the manner of their distribution detected by different lectins are considerably different.