[Design of the human tumor-associated VNTR(Muc1) antigen gene, fused with streptavidin, its expression in Escherichia coli. Study of properties of the hybrid protein]. / Konstruirovanie gena opukholeassotsiirovannogo antigena VNTR(Muc1) cheloveka, slitogo so streptavidinom, ego ékspressiia v Escherichia coli. Izuchenie svoistv gibridnogo belka.

A gene of human tumor-associated antigen VNTR(MUC1) bound to streptavidin, an expression plasmid, and a highly effective hybrid protein-producing strain were constructed. It was shown that the streptavidin leader peptide ensures an effective secretion of the hybrid protein into the periplasmic space of Escherichia coli cells. The hybrid protein was isolated in a homogeneous state and its immunogenic properties were studied.

[MHC restriction of the anti-allotypic response of T-lymphocytes in vitro]. / MHC-restriktsiia antiallotipicheskogo otveta T-limfotsitov in vitro.

In vitro MHC restriction of antigen-specific T-cell proliferation to Igk-Ib allotype of Igk chain has been studied in inbred August rats, using polyclonal and monoclonal antibodies to RT-1 molecules. Igk-1b-specific proliferation of immune T cells was completely abrogated by alloantiserum specific for RT-1c (August) molecules. Monoclonal antibodies (MAb) to RT-1B ("1-A-like") molecules inhibited markedly the response (about 70-80% inhibition), while anti-RT-ID ("I-E-like") MAb caused but weak inhibition (about 25%). Thus, the data obtained demonstrate RT-1Bc molecule as a main restriction element of Igk-1b-specific T-cell response.

[Comparative analysis of the specificity of poly- and monoclonal antiallotypic antibodies and allotype-recognizing T-lymphocytes in rats]. / Sravnitel'nyi analiz spetsifichnosti poli- i monoklonal'nykh antiallotipicheskikh antitel i allotipraspoznaiushchikh T-limfotsitov krys.

Using concurrent solid-phase radioimmunoassay, it has been shown that rat immunoglobulin k chain, Igk-lb allotype, is represented by a single serologically defined determinant. Anti-Igk-lb T cells do not recognize this determinant and poly- and monoclonal anti-Igk-lb antibodies show no influence on Igk-lb-specific proliferative T-cell response in vitro to IgG (Igk-lb)-pulsed splenic antigen-presenting cells. It is suggested that T cells recognize allotypic determinant(s) of processed Ig (Igk-lb) molecule.

[Ir-gene control of rat T-lymphocyte proliferation in response to Ig allotype]. / Ir-gennyi kontrol' proliferatsii T-limfotsitov krys na allotip Ig.

Antigen-induced T-cell proliferation in vitro was adapted to the estimation of antiallotypic response to Igk-Ib immunoglobulin k chain allotype of MSU/b and Fisher rats in WAG, August and FI (WAG X August) rats. August and FI rat T lymphocytes responded to Igk-Ib alloantigen with stimulation indexes (SI) 3.8-5.0 (high responders), while WAG rat T lymphocytes showed practically no response (SI 0.9-1.8--low responders). These results correlate well with our previous findings of Ir-gene-controlled antiallotypic in vitro reactions in these rat strains. Effective antigen presentation to FI anti-Igk-Ib T cells was observed only using Igk-Ib-pulsed antigen-presenting cells (APC) of August responders, but not of WAG non-responders. FI T cells responded well to PPD-pulsed APC of both rat strains. The data confirm Ir-Igk-Ib-gene control and, therefore, MHC-restriction of antiallotypic response in inbred rats.

[Comparative immunochemical analysis of various human leukocyte interferons]. / Sravnitel'nyi immunokhimicheskii analiz nekotorykh leikotsitarnykh interferonov cheloveka.

Using radioimmunological methods based on the use of mono- and polyclonal antibodies raised against interferon alpha A, it was shown that polyclonal antibodies quantitatively reacted not only with this protein, but also with interferons alpha F and alpha N, whereas all the variants of monoclonal antibodies studied reacted only with interferons alpha A and alpha N. Monoclonal antibodies 5A6, 11E9, 19C10, 258 and 268 are directed against overlapping epitopes of the interferon alpha A molecule, which simultaneously binds not more than two molecules of antibodies with different specificity. The correlation between immunochemical and biological activities of interferon alpha A during temperature denaturation and proteolytic degradation and its ability to form oligomeric complexes were investigated.

[Classes of rat lymphocyte surface immunoglobulins detected by enzymatic radioiodination]. / Klassy immunoglobulinov poverkhnosti limfotsitov krys, vyiavliaemye metodom énzimaticheskogo radioiodirovaniia

By means of enzymatic radioiodination, immunopre-cipitation and acrylamide gel electrophoresis in sodium dodecyl sulfate it was found that on the surface of normal rat splenocytes there were two main immunoglobulin classes--monomeric IgM and Ig (H2L2) which had a heavy chain larger than they phi, yet smallerthan the micron chain, differing from them by the antigenic properties. It is possible that this Ig class corresponds to the human IgD and the mouse IgD-like cell surface molecules. Small amounts of thedetected cell surface IgG may indicate both its cytophilic properties and the immunological status of the experimental animals.