[Influence of alimentary microelementosis on superoxide dismutase and glutathione peroxidase activities].

Studies of K(m) for glutathione peroxidase (GPx) and activities of superoxide dismutase and GPx were carried out in liver and erythrocytes of rats kept on either the normal semisynthetic diet or a high-fat diet with increased content of Cu, Zn, Mn, and Se. The diet containing microelement additions caused the increase in TBH affinity of liver and erythrocite GPx, as well as the decrease of liver SOD observed on the 14th day of the treatment of rats with the high-fat diet with additional increase of Cu, Zn, Mn, and Se.

[Estimation of organic and inorganic selenium bioavailability in experiments with growing rats].

There was conducted a comparative study of organic and inorganic selenium forms bioavailability and of an influence of said trace elements safety on growth parameters and glutathione peroxidase activity in male wister rats. The results obtained confirmed an advance of organic selenium use (as an enzymatic yeast hydrolysate) on comparison to it's inorganic form (sodium selenite).

[Specificity of activity antioxidative enzymes at protein deficiency and excessive content Cu, Zn, Mn, Se in the food].

The research of kinetic properties (Km u Vmax) of two enzymes: Superoxide Dismutase and Glutathione Peroxidase from rats liver and blood and lipid peroxidation induced by both a low protein diet (8%) and 2-fold concentration Cu, Zn, Mn, Se in diet. There was a change of Km and Vmax: the reduction of Km(GPH) was in liver at 28 d and the increase of Km(SOD) was in liver in group with 2-fold concentration Cu, Zn, Mn, Se in diet. The analysis of Km and Vmax of Superoxide Dismutase and Glutathione Peroxidase in different alimentary influence may be as one of methods for assessment individualization of diet therapy.

[Changes in kinetic properties of superoxide dismutase and glutathione peroxidase in the liver and erythrocytes from rats during deficiency of protein and additional dietary administration Cu, Zn, Mn and Se].

The kinetic parameters of liver and erythrocyte superoxide dismutase (SOD) and glutatione peroxidase (GP) have been investigated in rats kept under conditions of low protein diet (8%) and during addition of organic compounds containing Cu, Zn, Mn, Se. There were differences in kinetic parameters of hepatic and erythrocyte enzymes. After 2 and 4 weeks of maintenance of rats at a semi-synthetic diet there was an increase of Km value of liver and erythrocyte GP for tert-butyl hydroperoxide. After two weeks there was a decrease in Km of SOD for NADH-O2*- generating system. The low protein diet and consumption of Cu, Zn, Mn, Se caused a decrease of Km value of liver and erythrocytes GP and development of oxidative stress in liver as a result dietary administration Cu and Mn.