[Liver-induced fibrosis in young and old animals is accompanied by age-dependent changes in bone marrow cells.]

The hypothesis of dependence of the functional characterizations of bone marrow cells (BMC) - proliferation rate, direction proliferation, etc. - not only by the age of animals, but also on features of BMC microenvironment was verified. Two methods of changing the microenvironment were used. There were in vivo (induction of liver fibrosis in young and old animals) and in vitro (transfer of young and old animals BMC obtained in intact animals and animals with fibrosis into the same standard culture system). CuSO4-induced liver fibrosis and CCl4-induced liver fibrosis had a different effect on the ratio of cell types in the bone marrow in young and old animals. Thus, in young animals, regardless of the type of liver fibrosis inducer, the relative number of morphologically identifiable cell types decreased. This was accompanied by an increase in the number of identified cell types against the background of CuSO4-induced liver fibrosis and did not change against the background of CCl4-induced liver fibrosis in old animals. The proliferative activity of BMC isolated from old animals and transferred to an in vitro culture was superior to that of young animals. This is due to the large number of lymphocytes in the bone marrow of old animals by 167% and the specific composition and characteristics of the BMC microenvironment in old animals.

[Glycosaminoglycanes of cartilage tissue of rats after the mechanical trauma: the influence of low-molecular fraction from the cord blood and blood of dairy calves].

Influence of low-molecular fraction (to 5 kDa) from cord blood (FCB) compared with "Actovegin" derived from the blood of veal calves on the content of the matrix polysaccharide components from and proteins of the cartilage connecting tissue after the mechanical trauma was investigated. It is established, that intramuscular administration of FCB essentially stimulates the accumulation of the basic components of the matrix: hexosamine, uronic acid, hyaluronic acid, chondroitinsulfate and heparin, and also the most important components of structural proteins in the cartilage regenerate of the rat knee joint.