[Laurine fatty acids, medium fatty acids and triglycerides, hyperlipidemia, resistance to insulin, prevention of atherosclerosis and ateromatosis.]

Although the biochemistry of the positive effects of medium-chain fatty acids (FA) and triglycerides (TG) of the same name in vivo is not fully understood, food enriched with medium-chain LC and the same TG is effective in patients with type I diabetes, insulin resistance syndrome and in neurodegenerative pathology. Lauric C12 LC is half the FA in coconut oil. Residents of southeast Asia with constant use of coconut oil, have a low level of diseases of the cardiovascular system in the population. With a regulatory intake with food C12:0 laurin FA formed moderate ketosis and neuroprotective effect. Unlike long-chain LC, medium-chain TG cells are not deposited either in visceral fat cells, or in insulin-dependent adipocytes. Medium-chain fatty acids rapidly oxidize mitochondria; the formation of acetyl-CoA cells is used to form ketone bodies, activating thermogenesis in orange and brown adipocytes. Experiments with animals and observations in the clinic showed that taking medium-chain TG with food is more physiological than long-chain oils. This significantly increases the level of cholesterol in high-density lipoproteins. Food enriched with medium chain TG is optimal for increasing the ketone content in blood plasma, cerebrospinal fluid without limiting the carbohydrate content in food. The formation of excess ketone bodies by cells can be achieved by activating the metabolic transformations of medium-chain FAs, without fasting and preserving carbohydrates in food. Coconut oil has a positive effect on the cardiovascular system, preventing the formation of atherosclerosis and atheromatosis. Effective in the prevention of the pathology of the cardiovascular system is a decrease in food amounts of palmitic acid, an increase in oleic acid, polyene FA with a simultaneous increase in the proportion of medium-chain FA.

[The person in philogenesis is not (omnivores), but the herbivores with the carnivores past and the fuzzy future. Biological function of trophology (nutrition) in ontogenesis.]

According to the phylogenetic theory of general pathology, seven biological functions have been formed over billions of years. 1. biological function of trophology, nutrition; 2. homeostasis function; 3. biological function of endoecology; 4. function of adaptation; 5. function of the continuation of the species; 6. function of locomotion and 7. cognitive biological function, including intelligence. Millions of years in life consistently in the waters of several oceans, all the ancestors of man were carnivorous (Carnivores), fish-eating mammals. When the ocean retreated and the carnivorous (fish-eating) were on land, each individual privatized a "piece" of the ocean. Animals transformed it ito a pool of intercellular medium in vivo. The biological role of the late in the phylogeny of insulin is the formation of new biological functions in vivo. The action of insulin has transformed the carnivorous (fish-eating) ocean into herbivorous (Herbivores) species on land. There was it by synthesis in vivo from exogenous glucose of fatty acids (FA). Regulatory action of insulin was the directed conversion of exogenous glucose into ω-6 C18: 1 cis-oleic FA. Insulin late in phylogeny expressed the synthesis of new, conjugated enzymes: it is palmitoyl-CoA-elongase and stearyl-CoAdesaturase. Two enzymes synthesized FAs along the way: synthesized in situ de novo, from exogenous glucose, C16: 0 palmitic acid → C18: 0 stearic acid → ω-6 C18: 1 cis-oleic acid without accumulation of stearic FA. Insulin is not converted into an oleic FA exogenous palmitic acid from carnivorous food. On land, the action of insulin transformed the species Homo sapiens, into a herbivore, but with carnivorous, fish-eating, past. The idea of a person as omnivorous (Omnivor) - nonsense; such forms of nature did not form. Violation of the function of nutrition, the biological reaction of exotrophy (external nutrition), is the etiological and pathogenetic basis of the seven metabolic pandemics, the diseases of civilization. 1. Atherosclerosis and atheromatosis; 2. metabolic arterial hypertension; 3. metabolic syndrome; 4. obesity; 5. syndrome of insulin resistance; 6. non-alcoholic fatty liver disease and 7. endogenous hyperuricemia. The primary prevention of metabolic pandemics in the biological function of nutrition, in the biological reactions of exo-and endotrophy, will allow us to understand the theoretical bases and implementation of preventive actions that will determine the characteristics of nutrition in the future.

[The analytic quality in laboratory medicine: problems and perspectives (a lecture)].

The article considers the structure of analytical errors in clinical diagnostic laboratory analysis from the position of GOST R ISO 15189-2009 "Laboratories of medicine. Particular requirements to quality and competence". The key value of metrologic traceability of analyses is emphasized. The role of official standard patterns, control materials and statistical methods applied in quality analysis are discussed. The international experience and applied methodical procedures to implement requirements of ISO 15189 concerning validation and verification of analytical quality are presented. The approaches of protocols E3 23-A, ER 15-A2, N59-A in the sphere of USA laboratory medicine developed by the institute of clinical and laboratory standards are demonstrated. The review of referent patterns and methods is given. The problem of optimization of requirements to quality of production for laboratory diagnostic is discussed. The expedience of organization of the National institute of laboratory standards is substantiated.

[The practice of development and implementation of quality management systems in medical laboratories. The GOST R ISO 15189-2009 "medical laboratories. The detailed requirements to quality and competence". Particular difficulties of global nature].

The article discusses the methodological issues related to the implementation of international principles of standardization in the format of GOST R ISO 9001-2008 "Quality management systems. Requirements", GOST R ISO 15189-2009 "Medical laboratories. The detailed requirements to quality and competence" and GOST R ISO 18113.1-5 "Medical items for diagnostics in vitro. Information provided by manufacturer (marking)". This approach legibly assigns the responsibility concerning the support of metrological correctness of laboratory measurements. The lacking of both full-value public and sectorial normative documentation and coordinated positions of Rosstandard and Minzdrav of Russia on functioning of medical laboratories is noted.

[Introduction of quality systems into laboratory medicine: methodological aspects].

The paper considers the problems of introducing the quality management systems (QMS) according to GOST R ISO 9001-2001 in the health care facilities of different forms of property and departmental subordination. It gives examples of successfully putting QMS into practice in the companies that manufacture products for laboratory diagnosis in accordance with the Russian and international standardization principles. Methods are presented for training the staff of the facilities in the QMS principles and making a dialogue between laboratories and customers.

[Complex influence of chemo- and ozonotherapy on the immunity of patients with destructive pulmonary tuberculosis].

The impact of standardized antimycobacterial treatment versus a complex combination of chemo- and ozonotherapy on the T and B links and specific antituberculous immunity was studied in patients with destructive pulmonary tuberculosis. A course of ozonotherapy was performed in the early intensive phase of antimycobacterial therapy via endovascular injection of ozonized 0.9% NaCl solution. Ozonotherapy used in the early intensive phase of the standardized treatment in patients with destructive pulmonary tuberculosis results in normalization of the count of tuberculin-sensitized T cells and the number of PPD-L proliferating lymphocytes, promotes decreased tuberculin sensitivity, accelerates normalization of humoral immunity (IgM, IgE), and activates the proliferative capacity of T cells in the early periods.

[Effects of plasmapheresis with transfusion solutions on blood biochemical parameters and central hemodynamics in patients with chronic obstructive bronchitis]. / Vliianie plazmafereza s ispol'zovaniem raznykh transfuzionnykh rastvorov na biokhimicheskie pokazateli krovi i tsentral'nuiu gemodinamiku bol'nykh khronicheskim obstruktivnym bronkhitom.

The impact of plasmapheresis (P) using 0.9% physiological solutions of sodium chloride solution and 5% lactoprotein on some blood biochemical and central hemodynamic parameters was studied in 20 patients with chronic obstructive bronchitis. No matter what hemocorrector was used, P was found to promote reductions in blood medium-weight molecules of peptides, overall blood proteolytic activity, alpha 1-proteinase inhibitor, ceruloplasmin, transferrin and to increase lipid peroxidation. At the same time curative P using lactoprotein favours better central hemodynamics, which indicates its ability to correct postexfusion plasma volume when used in a dosage of 10 ml/kg body weight).

[Endotoxicosis in severe multiple trauma]. / Endotoksikoz pri tiazheloí sochetannoí travme.

The article touches upon the practical significance of methods of extracorporeal detoxication, pathogenesis of endotoxicosis, phases of its development. An analysis of data was made concerning treatment of 98 wounded and patients having injuries of three and more anatomical areas and who were in severe and critically severe states. Practical conclusions were made determining the expediency of using the methods of extracoprporeal detoxication in traumatic disease, special attention being given to optimization of functioning the detoxicating systems of organism.

[Pseudomembranous colitis and "intestinal sepsis" as a consequence of dysbacteriosis due to antibiotics]. / Psevdomebranoznyi kolit i "kishechnyi sepsis" vsledstvie disbakterioza, vyzvannogo antibiotikami.

Based upon 11 personal observations and data of literature the authors describe etiopathogenesis, clinical picture, diagnosis and treatment of pseudomembranous colitis. The recommended complex therapy resulted in the recovery of all the patients.