Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 190
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Bull Exp Biol Med ; 167(2): 233-236, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31236886

RESUMO

We studied the effect of NO synthesis blockade on the hydrouretic function and the state of renal interstitial matrix in WAG rats with normal blood vasopressin level and Brattleboro rats lacking endogenous vasopressin. In WAG rats, impaired osmotic concentration accompanied by an increase in the content of histochemically detectable glycosaminoglycans in the inner medullary interstitium was observed under conditions of NO synthesis blockade. In Brattleboro rats, NO synthesis inhibition, on the contrary, induced an antidiuretic response without significant changes in the renal extracellular matrix. These findings attested to a significant influence of NO on the urine concentration process. Morphofunctional changes in the kidneys under conditions of NO synthesis blockade are determined by blood vasopressin level.


Assuntos
Matriz Extracelular/metabolismo , Rim/metabolismo , Óxidos de Nitrogênio/metabolismo , Vasopressinas/sangue , Animais , Feminino , Glicosaminoglicanos/metabolismo , Ácido Hialurônico/metabolismo , Masculino , Ratos
2.
Khirurgiia (Mosk) ; (7): 23-26, 2018.
Artigo em Russo | MEDLINE | ID: mdl-29992921

RESUMO

Historical development of mitral valve surgery at Petrovsky Russian Research Center for Surgery is described in the article. Evolution of artificial heart valves design and development of surgical techniques are presented.


Assuntos
Implante de Prótese de Valva Cardíaca , Valva Mitral , Próteses Valvulares Cardíacas , Humanos , Insuficiência da Valva Mitral/cirurgia , Federação Russa
3.
Dokl Biol Sci ; 473(1): 43-45, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28508198

RESUMO

In the course of adaptation of the rat kidney collecting duct cells to hypo-osmotic medium, the organic anion transporter inhibitor probenecid reduced significantly the regulatory cell volume decrease in response to a hypotonic shock. Both probenecid and hypotonic shock delayed significantly the entry into a cell of the fluorescent dye calcein, which exists as anion at neutral pH. Thus, the organic osmolyte transport plays an important role in the regulatory decrease of the principal cell volume under the hypo-osmotic conditions.


Assuntos
Túbulos Renais Coletores/fisiologia , Rim/fisiologia , Pressão Osmótica/fisiologia , Equilíbrio Hidroeletrolítico/fisiologia , Animais , Tamanho Celular/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Rim/metabolismo , Túbulos Renais Coletores/metabolismo , Transportadores de Ânions Orgânicos Sódio-Independentes/metabolismo , Probenecid/farmacologia , Ratos
4.
J Transl Med ; 15(1): 54, 2017 03 03.
Artigo em Inglês | MEDLINE | ID: mdl-28257636

RESUMO

BACKGROUND: Endothelial and smooth muscle cells are considered promising resources for regenerative medicine and cell replacement therapy. It has been shown that both types of cells are heterogeneous depending on the type of vessels and organs in which they are located. Therefore, isolation of endothelial and smooth muscle cells from tissues relevant to the area of research is necessary for the adequate study of specific pathologies. However, sources of specialized human endothelial and smooth muscle cells are limited, and the search for new sources is still relevant. The main goal of our study is to demonstrate that functional endothelial and smooth muscle cells can be obtained from an available source-post-surgically discarded cardiac tissue from the right atrial appendage and right ventricular myocardium. METHODS: Heterogeneous primary cell cultures were enzymatically isolated from cardiac explants and then grown in specific endothelial and smooth muscle growth media on collagen IV-coated surfaces. The population of endothelial cells was further enriched by immunomagnetic sorting for CD31, and the culture thus obtained was characterized by immunocytochemistry, ultrastructural analysis and in vitro functional tests. The angiogenic potency of the cells was examined by injecting them, along with Matrigel, into immunodeficient mice. Cells were also seeded on characterized polycaprolactone/chitosan membranes with subsequent analysis of cell proliferation and function. RESULTS: Endothelial cells isolated from cardiac explants expressed CD31, VE-cadherin and VEGFR2 and showed typical properties, namely, cytoplasmic Weibel-Palade bodies, metabolism of acetylated low-density lipoproteins, formation of capillary-like structures in Matrigel, and production of extracellular matrix and angiogenic cytokines. Isolated smooth muscle cells expressed extracellular matrix components as well as α-actin and myosin heavy chain. Vascular cells derived from cardiac explants demonstrated the ability to stimulate angiogenesis in vivo. Endothelial cells proliferated most effectively on membranes made of polycaprolactone and chitosan blended in a 25:75 ratio, neutralized by a mixture of alkaline and ethanol. Endothelial and smooth muscle cells retained their functional properties when seeded on the blended membranes. CONCLUSIONS: We established endothelial and smooth muscle cell cultures from human right atrial appendage and right ventricle post-operative explants. The isolated cells revealed angiogenic potential and may be a promising source of patient-specific cells for regenerative medicine.


Assuntos
Prótese Vascular , Células Endoteliais/citologia , Miocárdio/citologia , Miócitos de Músculo Liso/citologia , Neovascularização Fisiológica , Desenho de Prótese , Animais , Diferenciação Celular , Proliferação de Células/efeitos dos fármacos , Separação Celular , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Quitosana/farmacologia , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/metabolismo , Células Endoteliais/ultraestrutura , Humanos , Camundongos SCID , Miócitos de Músculo Liso/efeitos dos fármacos , Miócitos de Músculo Liso/metabolismo , Miócitos de Músculo Liso/ultraestrutura , Neovascularização Fisiológica/efeitos dos fármacos , Molécula-1 de Adesão Celular Endotelial a Plaquetas/metabolismo , Poliésteres/farmacologia , Corpos de Weibel-Palade/metabolismo
5.
Bull Exp Biol Med ; 160(5): 691-4, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27021081

RESUMO

The effects of amiloride, epithelial sodium pump inhibitor, on the fast nongenomic effect of aldosterone in principal cells of an isolated segment of the distal portion of renal collecting tubes were studied in 10-day-old and adult rats. Fluorescent staining with Calcein AM showed various effects of amiloride (10(-5) M) on the stabilizing effect of aldosterone (10 nM) in hypotonic shock (280/140 mOsm/kg). Amiloride attenuated by 30% the effect of aldosterone on the amplitude of principal cell swelling in adult animals and almost completely abolished this effect in 10-day rats (p<0.05). These age-specific differences in the contribution of the distal portion of the collecting tube to the nongenomic effect of aldosterone did not depend on genetic heterogeneity of its α-subunit.


Assuntos
Aldosterona/farmacologia , Amilorida/farmacologia , Bloqueadores do Canal de Sódio Epitelial/farmacologia , Canais Epiteliais de Sódio/efeitos dos fármacos , Túbulos Renais Coletores/metabolismo , Animais , Canais Epiteliais de Sódio/genética , Transporte de Íons/efeitos dos fármacos , Masculino , Ratos , Ratos Wistar
6.
Ross Fiziol Zh Im I M Sechenova ; 102(2): 146-53, 2016 Feb.
Artigo em Russo | MEDLINE | ID: mdl-29671482

RESUMO

The involvement of wortmannin (10 -5 M), phosphatidyl inositol 3-kinase (PI3K) blocker, in the implementation of the rapid nongenomic aldosterone (10 nM) effects on the intracellular sodium (Na i +) and the principal cell volume of cortical collecting duct (CCD) of 10-day and adult rat kidney CCD was studied. Using fluorescence microscopy with intracellular dye Na Green and Calcein we found that wortmannin weakened the effect of aldosterone on the Na i + at low sodium in the extracellular medium (14 mM NaCl), and slowed the rate of reduction of the principal cell volume in the presence of aldosterone at the hypoosmotic shock (240/140 mOsm) since 10 days of age. The findings suggest the participation of phosphatidylinositol pathway in the fast nongenomic aldosterone effects (seconds and minutes) at the early stage of postnatal ontogenesis.


Assuntos
Aldosterona/metabolismo , Córtex Renal/metabolismo , Túbulos Renais Coletores/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Fatores Etários , Aldosterona/farmacologia , Animais , Tamanho Celular , Líquido Intracelular/metabolismo , Córtex Renal/efeitos dos fármacos , Córtex Renal/crescimento & desenvolvimento , Túbulos Renais Coletores/efeitos dos fármacos , Túbulos Renais Coletores/crescimento & desenvolvimento , Masculino , Pressão Osmótica , Inibidores de Fosfoinositídeo-3 Quinase , Ratos Wistar , Sódio/metabolismo
7.
Dokl Biochem Biophys ; 463: 247-50, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26335823

RESUMO

This paper presents the new priority data on the effect of genetically determined deficiency of endogenous vasopressin on the functioning of V2 receptor in the plasma membrane of the principal cells of the collection ducts of Brattleboro rats. It was found that 2-day desmopressin administration results in the activation of V2 receptor gene expression in Brattleboro rat, whereas in Wistar rats the content of mRNA did not change. In addition, short-term exposure of the collection ducts of the kidneys to the hormone led to V2 receptor internalization from the plasma membrane in Wistar rats, but had no effect on the protein content in Brattleboro rats.


Assuntos
Desamino Arginina Vasopressina/farmacologia , Receptores de Vasopressinas/metabolismo , Animais , Feminino , Masculino , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Ratos Brattleboro , Ratos Wistar , Receptores de Vasopressinas/genética , Especificidade da Espécie
10.
Bioorg Khim ; 40(6): 712-9, 2014.
Artigo em Russo | MEDLINE | ID: mdl-25895368

RESUMO

The expression of the total proteasome pool, immune proteasome subunits LMP2 and LMP7, TAP1 and TAP2 transporters, as well as RT1A molecule of MHC class I was investigated in the ascite Zajdela hepatoma at the 10th day after implantation into Brattleboro rats with the hereditary defect of hypothalamic arginine-vasopressin synthesis (AVP) and into WAG rats with normal AVP expression. In Zajdela hepatoma cells implanted into Brattleboro rats the 3-fold increase of the total proteasome pool and LMP2 level and 8-fold increase of the LMP7 level was detected by Western blotting as compared to those in WAG rats. Differences in the LMP2 and LMP7 expression suggest variations in their functions, namely the important role of LMP7 in anti-tumor immunity. The growth of Zajdela hepatoma in WAG rats was accompanied by the decreased level of total proteasome pool as well as immune proteasome expression as compared to those in Brattleboro rats during the regression of tumor. The analysis of TAP1 and TAP2 revealed the pronounced expression of these peptide transporters in Zajdela hepatoma cells implanted into Brattleboro and WAG rats. The expression level of RT1A molecule of MHC class I was increased 3 times in Zajdela hepatoma cells implanted into Brattleboro rats as compared to WAG rats. Moreover, flow cytometric analysis of CD4- and CD8-lymphocytes number in the spleen of Brattleboro and WAG rats was performed at the 10th day after implantation of Zajdela hepatoma. The increased number of CD4- and CD8-lymphocytes was observed in the spleen of Brattleboro as compared to WAG. The increased subpopulations of cytotoxic T-lymphocytes and T-helpers might promote the tumor regression in Brattleboro rats. The reduced populations of CD4- and CD8-lymphocytes in the spleen of WAG rats were accompanied by the splenomegaly and tumor progression. The data obtained suggest that AVP deficiency in Brattleboro rats leads to the increase of the immune proteasome and MHC class I expression in Zajdela hepatoma cells, resulting in tumor immunogenicity and its elimination by the adaptive immunity.


Assuntos
Arginina Vasopressina/genética , Cisteína Endopeptidases/biossíntese , Complexo de Endopeptidases do Proteassoma/imunologia , Membro 2 da Subfamília B de Transportadores de Cassetes de Ligação de ATP , Membro 3 da Subfamília B de Transportadores de Cassetes de Ligação de ATP , Transportadores de Cassetes de Ligação de ATP/metabolismo , Animais , Arginina Vasopressina/biossíntese , Ascite/imunologia , Ascite/metabolismo , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/imunologia , Carcinoma Hepatocelular/patologia , Cisteína Endopeptidases/imunologia , Regulação Neoplásica da Expressão Gênica/imunologia , Genes MHC Classe I/imunologia , Hipotálamo/metabolismo , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/imunologia , Neoplasias Hepáticas/patologia , Neoplasias Experimentais/genética , Neoplasias Experimentais/imunologia , Neoplasias Experimentais/patologia , Complexo de Endopeptidases do Proteassoma/biossíntese , Complexo de Endopeptidases do Proteassoma/genética , Ratos
11.
Bull Exp Biol Med ; 155(5): 615-7, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24288722

RESUMO

The mechanism of aldosterone effects (10 nM) on the volume of the principal cells of cortical collecting ducts after hypoosmotic stress (280/140 mOsm/kg) was studied using fluorescence microscopy. The experiments with intracellular fluorescent dye calcein showed that aldosterone significantly decreases the amplitude and rate of increase in principal cell volume during hypoosmotic stress. Epithelial sodium channel blocker amiloride (10(-5)M) significantly attenuated the effects of aldosterone on the amplitude and rate of changes in cell volume. The obtained data attest to the contribution of epithelial sodium channel to the realization of rapid non-genomic effects of aldosterone on the amplitude and rate of changes in volume of the principal cells of cortical collecting ducts in rat kidney after hypoosmotic stress.


Assuntos
Aldosterona/farmacologia , Tamanho Celular/efeitos dos fármacos , Canais Epiteliais de Sódio/metabolismo , Túbulos Renais Coletores/efeitos dos fármacos , Sódio/metabolismo , Amilorida/farmacologia , Animais , Bloqueadores do Canal de Sódio Epitelial/farmacologia , Fluoresceínas , Corantes Fluorescentes , Transporte de Íons/efeitos dos fármacos , Túbulos Renais Coletores/citologia , Túbulos Renais Coletores/fisiologia , Microscopia de Fluorescência , Concentração Osmolar , Pressão Osmótica , Ratos , Ratos Wistar , Técnicas de Cultura de Tecidos
13.
Izv Akad Nauk Ser Biol ; (1): 99-102, 2013.
Artigo em Russo | MEDLINE | ID: mdl-23662467

RESUMO

The dynamics of expression of the RT1A antigen of the class I major histocompatibility complex (MHC) in a Walker 256 tumor after its transplantation into Brattleboro rats with a genetic defect of Arginine-Vasopressin synthesis in the hypothalamus was studied. Expression of the RT1A antigen was detected by means of Western-blotting and flow cytometry in the tumor cells on the 14th-17th days after transplantation. In addition, a simultaneous increase in the portion of cells that express the RT1A antigen and in the level of its expression per cell was observed. It is presupposed that at a deficiency of Arginine-Vasopressin, a renewal of expression of the class I MHC antigens, which results in an increase of immunogenicity of this tumor and regression, occurs in the Walker 256 tumor in the Brattleboro rats.


Assuntos
Carcinoma 256 de Walker , Antígenos de Histocompatibilidade Classe I/metabolismo , Antígenos de Histocompatibilidade , Animais , Arginina/biossíntese , Arginina/genética , Arginina/imunologia , Carcinoma 256 de Walker/genética , Carcinoma 256 de Walker/imunologia , Carcinoma 256 de Walker/metabolismo , Citometria de Fluxo , Antígenos de Histocompatibilidade/genética , Antígenos de Histocompatibilidade/imunologia , Antígenos de Histocompatibilidade/metabolismo , Antígenos de Histocompatibilidade Classe I/imunologia , Ratos , Ratos Brattleboro , Vasopressinas/biossíntese , Vasopressinas/imunologia , Vasopressinas/metabolismo
15.
Ross Fiziol Zh Im I M Sechenova ; 98(6): 777-81, 2012 Jun.
Artigo em Russo | MEDLINE | ID: mdl-23013015

RESUMO

Effect of dehydration and arginine vasopressin treatment (Arg-VP Sigma, USA, 50 ng/100 g b.wt. ip.) on the blood plasma hyaluronidase activity in Wistar rats was studied. It was found that the pH optimum of the enzyme activity was in the range of 3.5-3.7 that is characteristic for the hyaluronidase type 1. Water deprivation for 1 day was followed by significant increase in the blood plasma hyaluronidase activity. The same response was observed under vasopressin treatment. The possible role of increased plasma hyaluronidase activity in the regulation of water balance is discussed.


Assuntos
Antidiuréticos/administração & dosagem , Arginina Vasopressina/administração & dosagem , Desidratação/enzimologia , Hialuronoglucosaminidase/metabolismo , Animais , Antidiuréticos/sangue , Arginina Vasopressina/sangue , Desidratação/sangue , Concentração de Íons de Hidrogênio , Isoenzimas/metabolismo , Masculino , Concentração Osmolar , Ratos , Ratos Wistar , Privação de Água/fisiologia , Equilíbrio Hidroeletrolítico/efeitos dos fármacos , Equilíbrio Hidroeletrolítico/fisiologia
16.
Bull Exp Biol Med ; 152(6): 728-30, 2012 Apr.
Artigo em Inglês, Russo | MEDLINE | ID: mdl-22803175

RESUMO

The characteristics of the osmotic concentration system under conditions of sodium diclofenac treatment were studied in Wistar rats with normally functioning vasopressin gene and homozygotic Brattleboro rats completely lacking endogenous vasopressin. Blockade of prostaglandin synthesis in rats with different neurohypophyseal status stimulated urinary osmolality to a different degree. Different contribution of sodium cations and urea to osmotic concentration was revealed.


Assuntos
Inibidores de Ciclo-Oxigenase/administração & dosagem , Diclofenaco/administração & dosagem , Rim/efeitos dos fármacos , Neuro-Hipófise/efeitos dos fármacos , Antagonistas de Prostaglandina/administração & dosagem , Animais , Cátions Monovalentes , Rim/metabolismo , Rim/fisiopatologia , Concentração Osmolar , Neuro-Hipófise/metabolismo , Neuro-Hipófise/fisiopatologia , Prostaglandinas/metabolismo , Ratos , Ratos Brattleboro , Ratos Wistar , Sódio/urina , Ureia/urina , Vasopressinas/deficiência , Vasopressinas/genética , Equilíbrio Hidroeletrolítico/efeitos dos fármacos
17.
Ross Fiziol Zh Im I M Sechenova ; 98(3): 373-80, 2012 Mar.
Artigo em Russo | MEDLINE | ID: mdl-22645946

RESUMO

Activation of the epidermal growth factor receptors (EGFR) plays an important role in the regulation of the kidney blood circulation and ion transport. The mRNA content of the epidermal growth factor (Egf) and its receptor (Egfr) genes were studied in the cortical and medullar parts of the kidney of rats with stress sensitive arterial hypertension (ISIAH strain) and normotensive WAG rats. There were no differences in the Egf expression between the rat strains at rest and under emotional stress in both the cortical and medullary kidney zones. As for Egfr transcription, it was significantly higher in the cortical and medullary parts of the ISIAH kidneys at rest as compared to the control WAG. Emotional stress induced no significant changes in the Egfr transcription in the cortical kidney parts of both rat strains. But prominent enhancement of Egfr transcription after emotional stress was observed in the kidney medulla of the WAG rats: it became the same as in the ISIAH rats at rest. It was suggested that the stress hormones might be involved in the regulation of Egfr transcription, and this connection between stress and Egfr gene activity might play a significant role in the kidney involvement in pathogenesis of stress sensitive arterial hypertension.


Assuntos
Córtex Suprarrenal/metabolismo , Medula Suprarrenal/metabolismo , Fator de Crescimento Epidérmico/metabolismo , Receptores ErbB/metabolismo , Rim/metabolismo , Estresse Psicológico , Animais , Pressão Sanguínea , Fator de Crescimento Epidérmico/genética , Receptores ErbB/genética , Regulação da Expressão Gênica , Hipertensão/metabolismo , Hipertensão/patologia , Masculino , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos
19.
Ross Fiziol Zh Im I M Sechenova ; 97(3): 235-62, 2011 Mar.
Artigo em Russo | MEDLINE | ID: mdl-21675200

RESUMO

Body water balance is regulated by vasopressin, and multiple studies now have revealed essential role of aquaporins in this process. This review summarizes recent advances related to molecular events underlying the short-term control of water permeability which occurs via vesicular trafficking of AQP2 to the cell apical membrane, and long-term control through changes in the gene expression and the abundance of AQP-2, -3 and -4. The action of vasopressin on water permeability is counterbalanced by several factors such as prostaglandin E2, bradykinin, dopamin, endotelin-1 and others. Changes in AQP-2 expression / trafficking are of particular importance in pathological conditions characterized by both diluting and concentration defect of renal function.


Assuntos
Diurese/fisiologia , Túbulos Renais Coletores/fisiologia , Vasopressinas/fisiologia , Animais , Aquaporinas/metabolismo , Aquaporinas/fisiologia , Água Corporal/metabolismo , Permeabilidade da Membrana Celular , Humanos , Túbulos Renais Coletores/metabolismo , Transporte Proteico , Vasopressinas/metabolismo , Vasopressinas/farmacologia , Equilíbrio Hidroeletrolítico/fisiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...