Sub-acute exposure to Sudan IV-adulterated palm oil induces oxidative stress and represses the expression of Nrf2 and antioxidant genes in male albino rats.

This study investigated the effects of Sudan IV dye (S4D) on antioxidant biomarkers using palm oil adulterated with S4D. Thirty male albino rats were grouped into five (n = 6); Normal control, palm oil (PO), PO + S4D (100 mg/kg), PO + S4D (250 mg/kg), and S4D (250 mg/kg) for 21 days. Oxidative stress biomarkers were assessed in the serum, liver, and kidneys. Exposure to S4D (alone and in adulterated PO) occasioned significant depletions in the activities of SOD, CAT, and GPx, as well as GSH levels in the assessed compartments. Contrastingly, the levels of NO and MDA were significantly (p < 0.05) increased in the serum, liver, and kidney of rats exposed to PO + S4D (both doses) and S4D (250 mg/kg) when compared to control rats. Further, the expressions of the genes coding for CAT, GPx-1, GSR, and Nrf-2 were significantly (p < 0.05) down-regulated, relative to ß-actin, in groups exposed to S4D compared to the control. Interestingly, these parameters were not significantly different (p > 0.05) in the unadulterated PO-exposed rats compared to the control. These results show that S4D depleted the antioxidant capacities, while potentiating the generation of reactive species and oxidative damage. This study provides useful information on the oxidative mechanisms associated with consumption of S4D-containing consumer products.

Anti-hyperglycemic potential of Hyptis verticillata jacq in streptozotocin-induced diabetic rats.

Uncontrolled hyperglycaemia and oxidative stress have been implicated in the pathophysiology of diabetes mellitus. Hyptis verticillata is reportedly explored traditionally for its therapeutic benefits. Resulting from the paucity of information on the anti-hyperglycaemic potential of this plant, the present study assessed the anti-hyperglycaemic activity of H. verticillata leaf extract. Fifty-four albino Wistar rats were divided into two main groups consisting of diabetic and non-diabetic rats. The diabetic and non-diabetic rats were either treated with oral doses of metformin (500 mg/kg b.w.), quercetin (10 mg/kg b.w.), ethanol extract of H. verticillata leaf (low dose: 250 mg/kg b.w.) or H. verticillata (high dose: 500 mg/kg b.w.) for 28 days. Results showed significantly decreased body weight, increased fasting blood glucose (FBG) and glycated haemoglobin (HbA1c) levels, decreased pancreatic islet area and ß-cell number in the diabetic untreated group, relative to normal control. H. verticillata - treated diabetic rats showed decreased FBG and HbA1c, increased body weight, pancreatic islet area and ß-cell number, comparable to the effects of metformin. GCMS analysis of H. verticillata showed the presence of ten bioactive volatile compounds, with squalene which possess strong antioxidant, hypoglycaemic and hypotriglyceridemic effects, as the most abundant. We therefore conclude that H. verticillata has anti-hyperglycaemic properties.

Dichlorvos toxicity: A public health perspective.

Pesticides are used in agriculture and in domestic pest control. Dichlorvos, an organophosphate, is a predominant pesticide used in domestic insect control in developing countries. Acute and prolonged exposure may lead to death, genotoxic, neurological, reproductive, carcinogenic, immunological, hepatic, renal, respiratory, metabolic, dermal and other systemic effects. Its toxicity is due to the ability of the compound to inhibit acetyl cholinesterase at cholinergic junction of the nervous system. This study is a review of the toxicological effects of dichlorvos in a public health perspective.