1.
Carbohydr Res
; 345(17): 2458-63, 2010 Nov 22.
Artigo
em Inglês
| MEDLINE
| ID: mdl-20934176
RESUMO
To determine the structural specificity of the glycosyl acceptor of the transglycosylation reaction using endo-ß-N-acetylglucosaminidase (ENGase) (EC 3.2.1.96) from Mucor hiemalis (Endo-M), several acceptor derivatives were designed and synthesized. The narrow regions of the 1,3-diol structure from the 4- to 6-hydroxy functions of GlcNAc were found to be essential for the transglycosylation reaction using Endo-M. Furthermore, it was determined that Endo-M strictly recognizes a 1,3-diol structure consisting of primary and secondary hydroxyl groups.