[Rapid determination of antibiotics resistance in clinico-surgical practice on the basis of radioactive carbon dioxide measurements]. / Schnellbestimmung der Antibiotikum-Resistenz in der chirurgisch-klinischen Praxis auf Grund der Messung von radioaktivem Kohlendioxyd.

The radiometric method is founded on the measurement of the 14CO2 which has gone free. This method was worked out of our team. It is a prompt sensitive and quantitative procedure for the control of antibiotic effect on bacteria. The method is a support in critical clinical situations. The prompt establishment of the antibiotic sensitivity can be live-saving. The empirics descended from 312 surgical patients. The antibiotical sensitivity of secretion samples is to be obtained within 4-6 hours. The results show in comparison with the classic bacteriological examinations five per cent less deviation. At the same time the computer analysis enables a finer distinction. 22 variable findings are possible within the categories: sensible, middling sensible and resistant. In that way the tested antibiotics are classified in an order of rank. The apparatus for the examination is producible without particular expenses. The measurements can be carried out at each hospital or clinical laboratory.

Changes in tumour cell selenite (75Se) affinity due to irradiation.

The uptake of 75Se sodium selenite in the liver and tumour tissue of irradiated Wistar rats was studied by radiation measuring methods and radiochromatography. In in vitro experiments liver slices were incubated in selenite solution following irradiation of the liver. The uptake in proteins diminished by 11% as compared with the unirradiated rats. The in vivo 75Se uptake in Ehrlich ascites tumour cells was 71% of that of the controls, when the selenite was administered intravenously after irradiation. The in vivo accumulation of selenite in proteins of Guérin tumours after irradiation was 62% of that in the controls. The radiochromatogram pattern showed a distinct difference between the irradiated and unirradiated tumours. These results are in accord with the clinical 75Se-scintigraphy findings, which show a clear decrease in Se-accumulation after irradiation.