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1.
Sensors (Basel) ; 23(9)2023 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-37177702

RESUMO

Speech processing algorithms, especially sound source localization (SSL), speech enhancement, and speaker tracking are considered to be the main fields in this application. Most speech processing algorithms require knowing the number of speakers for real implementation. In this article, a novel method for estimating the number of speakers is proposed based on the hive shaped nested microphone array (HNMA) by wavelet packet transform (WPT) and 2D sub-band adaptive steered response power (SB-2DASRP) with phase transform (PHAT) and maximum likelihood (ML) filters, and, finally, the agglomerative classification and elbow criteria for obtaining the number of speakers in near-field scenarios. The proposed HNMA is presented for aliasing and imaging elimination and preparing the proper signals for the speaker counting method. In the following, the Blackman-Tukey spectral estimation method is selected for detecting the proper frequency components of the recorded signal. The WPT is considered for smart sub-band processing by focusing on the frequency bins of the speech signal. In addition, the SRP method is implemented in 2D format and adaptively by ML and PHAT filters on the sub-band signals. The SB-2DASRP peak positions are extracted on various time frames based on the standard deviation (SD) criteria, and the final number of speakers is estimated by unsupervised agglomerative clustering and elbow criteria. The proposed HNMA-SB-2DASRP method is compared with the frequency-domain magnitude squared coherence (FD-MSC), i-vector probabilistic linear discriminant analysis (i-vector PLDA), ambisonics features of the correlational recurrent neural network (AF-CRNN), and speaker counting by density-based classification and clustering decision (SC-DCCD) algorithms on noisy and reverberant environments, which represents the superiority of the proposed method for real implementation.

2.
Sensors (Basel) ; 23(3)2023 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-36772574

RESUMO

This article proposes a novel method for detecting coronavirus disease 2019 (COVID-19) in an underground channel using visible light communication (VLC) and machine learning (ML). We present mathematical models of COVID-19 Deoxyribose Nucleic Acid (DNA) gene transfer in regular square constellations using a CSK/QAM-based VLC system. ML algorithms are used to classify the bands present in each electrophoresis sample according to whether the band corresponds to a positive, negative, or ladder sample during the search for the optimal model. Complexity studies reveal that the square constellation N=22i×22i,(i=3) yields a greater profit. Performance studies indicate that, for BER = 10-3, there are gains of -10 [dB], -3 [dB], 3 [dB], and 5 [dB] for N=22i×22i,(i=0,1,2,3), respectively. Based on a total of 630 COVID-19 samples, the best model is shown to be XGBoots, which demonstrated an accuracy of 96.03%, greater than that of the other models, and a recall of 99% for positive values.


Assuntos
COVID-19 , Humanos , COVID-19/diagnóstico , Algoritmos , Comunicação , Luz , Aprendizado de Máquina
3.
Sensors (Basel) ; 24(1)2023 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-38202893

RESUMO

This paper proposes a new system based on the Industrial Internet of Things (IIoT) for the monitoring of Mobile Health (m-Health) of workers in the underground mining industry. The proposed architecture uses a hybrid model in data transmission. Visible Light Communication (VLC) is used for downlink because of its narrow coverage, which aids in worker positioning. Radio frequency (RF) communication technology is used to send data for primary vital signs in the uplink, which is more efficient in transmission and is a viable solution according to the problem raised. The results obtained in terms of coverage and transmission for the downlink and uplink links show the feasibility of implementing the proposed system.

4.
Pharmaceutics ; 14(11)2022 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-36365241

RESUMO

Synthetic double-stranded small interfering RNAs (siRNAs) mimic interference RNAs (RNAi) and can bind target mRNAs with a high degree of specificity, leading to selective knockdown of the proteins they encode. However, siRNAs are very labile and must be both protected and transported by nanoparticles to be efficiently delivered into cells. In this work, we used a Janus-type polycationic amphiphilic ß-cyclodextrin derivative to efficiently transfect siRNAs targeting mRNAs encoding mitogen-activated protein kinase (p42-MAPK) or Ras homolog enriched in brain (Rheb) into different cancer cell lines as well as astrocytes. We took advantage of this high transfection efficiency to simultaneously knock down p42-MAPK and Rheb to boost docetaxel (DTX)-mediated toxicity in two human prostate cancer cell lines (LNCaP and PC3). We found that double knockdown of p42-MAPK and Rheb increased DTX-toxicity in LNCaP but not in PC3 cells. However, we also observed the same effect when scramble siRNA was used, therefore pointing to an off-target effect. Indeed, we found that the siRNA we used in this work induced toll-like receptor 3 activation, leading to ß-interferon production and caspase activation. We believe that this mechanism could be very useful as a general strategy to elicit an immune response against prostate cancer cells.

5.
Entropy (Basel) ; 24(11)2022 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-36359600

RESUMO

Visible light communication (VLC) is considered an enabling technology for future 6G wireless systems. Among the many applications in which VLC systems are used, one of them is harsh environments such as Underground Mining (UM) tunnels. However, these environments are subject to degrading environmental and intrinsic challenges for optical links. Therefore, current research should focus on solutions to mitigate these problems and improve the performance of Underground Mining Visible Light Communication (UM-VLC) systems. In this context, this article presents a novel solution that involves an improvement to the Angle Diversity Receivers (ADRs) based on the adaptive orientation of the Photo-Diodes (PDs) in terms of the Received Signal Strength Ratio (RSSR) scheme. Specifically, this methodology is implemented in a hemidodecahedral ADR and evaluated in a simulated UM-VLC scenario. The performance of the proposed design is evaluated using metrics such as received power, user data rate, and bit error rate (BER). Furthermore, our approach is compared with state-of-the-art ADRs implemented with fixed PDs and with the Time of Arrival (ToA) reception method. An improvement of at least 60% in terms of the analyzed metrics compared to state-of-the-art solutions is obtained. Therefore, the numerical results demonstrate that the hemidodecahedral ADR, with adaptive orientation PDs, enhances the received optical signal. Furthermore, the proposed scheme improves the performance of the UM-VLC system due to its optimum adaptive angular positioning, which is completed according to the strongest optical received signal power. By improving the performance of the UM-VLC system, this novel method contributes to further consideration of VLC systems as potential and enabling technologies for future 6G deployments.

6.
Sensors (Basel) ; 22(7)2022 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-35408098

RESUMO

Underground Mining (UM) is a hostile industry that generally requires a wireless communication system as a cross-cutting axis for its optimal operation. Therefore, in the last five years, it has been shown that, in addition to radio-frequency-based communication links, wireless optical communications, such as Visible Light Communication (VLC), can be applied to UM environments. The application of VLC systems in underground mines, known as UM-VLC, must take into account the unique physical features of underground mines. Among the physical phenomena found in underground mines, the most important ones are the positioning of optical transmitters and receivers, irregular walls, shadowing, and a typical phenomenon found in tunnels known as scattering, which is caused by the atmosphere and dust particles. Consequently, it is necessary to use proper dust particle distribution models consistent with these scenarios to describe the scattering phenomenon in a coherent way in order to design realistic UM-VLC systems with better performance. Therefore, in this article, we present an in-depth study of the interaction of optical links with dust particles suspended in the UM environment and the atmosphere. In addition, we analytically derived a hemispherical 3D dust particle distribution model, along with its main statistical parameters. This analysis allows to develop a more realistic scattering channel component and presents an enhanced UM-VLC channel model. The performance of the proposed UM-VLC system is evaluated using computational numerical simulations following the IEEE 802.1.5.7 standard in terms of Channel Impulse Response (CIR), received power, Signal-to-Noise-Ratio (SNR), Root Mean Square (RMS) delay spread, and Bit Error Rate (BER). The results demonstrate that the hemispherical dust particle distribution model is more accurate and realistic in terms of the metrics evaluated compared to other models found in the literature. Furthermore, the performance of the UM-VLC system is negatively affected when the number of dust particles suspended in the environment increases.

7.
Front Oncol ; 12: 786739, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35198439

RESUMO

Metastatic tumors with moderate radiosensitivity account for most cancer-related deaths, highlighting the limitations of current radiotherapy regimens. The xCT-inhibitor sulfasalazine (SAS) sensitizes cancer cells to radiotherapy by blocking cystine uptake via the xCT membrane antiporter, and thereby glutathione (GSH) synthesis protecting against radiation-induced oxidative stress. The expression of xCT in multiple tumor types implies it as a target generic to cancer rather than confined to few subtypes. However, SAS has limited clinical potential as a radiosensitizer due to side effects and low bioavailability. Using SAS as a starting point, we previously developed synthetic xCT-inhibitors through scaffold hopping and structure optimization aided by structure-activity relationship analysis (SAR). Notably, the compound DC10 exhibited inhibition of GSH synthesis. In this study, we validated DC10 as a radiosensitizer in the xCT-expressing cancer cell lines A172, A375 and MCF7, and mice harboring melanoma xenografts. After DC10 treatment, we measured 14C-cystine uptake in the cancer cells using liquid scintillation counting, and intracellular GSH levels and reactive oxygen species (ROS) using luminescence assays. We performed immunoblotting of H2AX and ATM to assess DNA damage after treatment with DC10 and radiotherapy. We then assessed the effect of adding DC10 to radiation upon cancer cell colony formation. Blood samples from mice treated with DC10 underwent biochemical analysis to assess toxicity. Finally, mice with A375 melanomas in the flank, received DC10 and radiotherapy in combination, as monotherapies or no treatment. Notably, DC10 reduced cystine uptake and GSH synthesis and increased ROS levels in a dose-dependent manner. Furthermore, DC10 interacted synergistically with radiation to increase DNA damage and reduce tumor cell colony formation. Mice receiving DC10 were clinically unaffected, whereas blood samples analysis to assess bone marrow suppression, liver or kidney toxicity revealed no significant differences between treated mice and untreated controls. Importantly, DC10 potentiated the anti-tumor efficacy of radiation in mice with melanoma xenografts. We conclude that DC10 is well tolerated and acts as a radiosensitizer by inhibiting cystine uptake, leading to GSH depletion and increased oxidative stress. Our findings demonstrate the feasibility of using synthetic xCT-inhibitors to overcome radioresistance.

8.
Sensors (Basel) ; 22(3)2022 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-35161757

RESUMO

Multiple simultaneous sound source localization (SSL) is one of the most important applications in the speech signal processing. The one-step algorithms with the advantage of low computational complexity (and low accuracy), and the two-step methods with high accuracy (and high computational complexity) are proposed for multiple SSL. In this article, a combination of one-step-based method based on the generalized eigenvalue decomposition (GEVD), and a two-step-based method based on the adaptive generalized cross-correlation (GCC) by using the phase transform/maximum likelihood (PHAT/ML) filters along with a novel T-shaped circular distributed microphone array (TCDMA) is proposed for 3D multiple simultaneous SSL. In addition, the low computational complexity advantage of the GCC algorithm is considered in combination with the high accuracy of the GEVD method by using the distributed microphone array to eliminate spatial aliasing and thus obtain more appropriate information. The proposed T-shaped circular distributed microphone array-based adaptive GEVD and GCC-PHAT/ML algorithms (TCDMA-AGGPM) is compared with hierarchical grid refinement (HiGRID), temporal extension of multiple response model of sparse Bayesian learning with spherical harmonic (SH) extension (SH-TMSBL), sound field morphological component analysis (SF-MCA), and time-frequency mixture weight Bayesian nonparametric acoustical holography beamforming (TF-MW-BNP-AHB) methods based on the mean absolute estimation error (MAEE) criteria in noisy and reverberant environments on simulated and real data. The superiority of the proposed method is presented by showing the high accuracy and low computational complexity for 3D multiple simultaneous SSL.


Assuntos
Localização de Som , Algoritmos , Teorema de Bayes , Ruído , Som
9.
Sensors (Basel) ; 20(3)2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32023989

RESUMO

In this manuscript we propose a hybrid Visible Light Communication and Radio Frequency (VLC-RF) scheme for the implementation of a portable Phaser Measurement Unit (PMU) for deep underground tunnels. Through computer simulations and laboratory measurements we are capable of providing Coordinated Universal Time (UTC) to the PMUs, as well as high accuracy positioning in a Global Positioning System (GPS) denied environment. The estimated PMU position, time stamp, and electrical power system measurements are sent to a central monitoring station using a radio frequency uplink with a data rate of hundreds of Kbps. Simulations and experimental measurements show that the proposed scheme can be used to control a large number of VLC-RF PMU devices inside a tunnel. The tests demonstrate the viability of the hybrid prototype, which will improve performance compared to commercial PMUs that lack these features.

10.
Sensors (Basel) ; 20(2)2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31936434

RESUMO

This paper proposes two solutions based on angle diversity receivers (ADRs) to mitigate inter-cell interference (ICI) in underground mining visible light communication (VLC) systems, one of them is a novel approach. A realistic VLC system based on two underground mining scenarios, termed as mining roadway and mine working face, is developed and modeled. A channel model based on the direct component in line-of-sight (LoS) and reflections of non-line-of-sight (NLoS) links is considered, as well as thermal and shot noises. The design and mathematical models of a pyramid distribution and a new hemi-dodecahedral distribution are addressed in detail. The performances of these approaches, accompanied by signal combining schemes, are evaluated with the baseline of a single photo-diode in reception. Results show that the minimum lighting standards established in both scenarios are met. As expected, the root-mean-square delay spread decreases as the distance between the transmitters and receivers increases. Furthermore, the hemi-dodecahedron ADR in conjunction with the maximum ratio combining (MRC) scheme, presents the best performance in the evaluated VLC system, with a maximum user data rate of 250 Mbps in mining roadway and 120 Mbps in mine working face, received energy per bit/noise power of 32 dB and 23 dB, respectively, when the bit error rate corresponds to 10 - 4 , and finally, values of 120 dB in mining roadway and 118 dB in mine working face for signal-to-interference-plus-noise ratio are observed in a cumulative distribution function.

12.
Nanomedicine (Lond) ; 12(20): 2533-2554, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28952878

RESUMO

Glioblastoma (GBM) is a very aggressive CNS tumor with poor prognosis. Current treatment lacks efficacy indicating that new therapeutic approaches are needed. One of these new approaches is based on the use of nanoparticles (NPs) to deliver different cargos (antitumoral drugs or genetic materials) to tumoral cells. This review covers the signaling pathways altered in GBM cells to understand the rationale behind choosing new therapeutic targets and recent advances in the use of different NPs to deliver to GBM cells, both in vitro and in vivo, different therapeutic molecules. A special focus is placed on the effect of NPs on orthotopic brain tumors since this animal model represents the optimal model for translational purposes.


Assuntos
Antineoplásicos/química , Antineoplásicos/farmacologia , Neoplasias do Sistema Nervoso Central/tratamento farmacológico , Portadores de Fármacos/química , Glioblastoma/tratamento farmacológico , Nanopartículas/química , Animais , Antineoplásicos/uso terapêutico , Barreira Hematoencefálica/metabolismo , Linhagem Celular Tumoral , Liberação Controlada de Fármacos , Humanos , Terapia de Alvo Molecular , Polímeros/química , Transdução de Sinais
13.
Nanomedicine (Lond) ; 12(13): 1607-1621, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28621615

RESUMO

AIM: To study the structural requirements that a cyclooligosaccharide-based nanoparticle must fulfill to be an efficient siRNA transfection vector. MATERIALS & METHODS: siRNA protection from degradation by RNAses, transfection efficiency and the thermodynamic parameters of the nanoparticle/siRNA interactions were studied on pairs of amphiphilic molecules using biochemical techniques and molecular dynamics. RESULTS: The lower the siRNA solvent accessible surface area in the presence of the nanoparticle, higher the protection from RNAse-mediated degradation in the corresponding nanocomplex; a moderate nanoparticle/siRNA binding energy value further facilitates reversible complexation and binding to the target cellular mRNA. CONCLUSION: The use, in advance, of these parameters will provide a useful indication of the potential of a molecular nanoparticle as siRNA transfecting vector.


Assuntos
Nanopartículas/química , Oligossacarídeos/química , RNA Interferente Pequeno/genética , Transfecção/métodos , Animais , Técnicas de Cultura de Células , Linhagem Celular , Corantes Fluorescentes , Técnicas de Silenciamento de Genes , Humanos , Interações Hidrofóbicas e Hidrofílicas , Simulação de Dinâmica Molecular , Ratos
14.
PLoS One ; 11(11): e0165704, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27832093

RESUMO

Glioblastomas are the most common malignant primary brain tumours in adults and one of the most aggressive and difficult-to-treat cancers. No effective treatment exits actually for this tumour and new therapeutic approaches are needed for this disease. One possible innovative approach involves the nanoparticle-mediated specific delivery of drugs and/or genetic material to glioblastoma cells where they can provide therapeutic benefits. In the present work, we have synthesised and characterised several second generation amphiphilic polylysine dendrons to be used as siRNA carriers. We have found that, in addition to their siRNA binding properties, these new compounds inhibit the proliferation of two glioblastoma cell lines while being nontoxic for non-tumoural central nervous system cells like neurons and glia, cell types that share the anatomical space with glioblastoma cells during the course of the disease. The selective toxicity of these nanoparticles to glioblastoma cells, as compared to neurons and glial cells, involves mitochondrial depolarisation and reactive oxygen species production. This selective toxicity, together with the ability to complex and release siRNA, suggests that these new polylysine dendrons might offer a scaffold in the development of future nanoparticles designed to restrict the proliferation of glioblastoma cells.


Assuntos
Antineoplásicos/farmacologia , Neoplasias Encefálicas/tratamento farmacológico , Proliferação de Células/efeitos dos fármacos , Dendrímeros/farmacologia , Portadores de Fármacos/farmacologia , Glioblastoma/tratamento farmacológico , Polilisina/farmacologia , Animais , Antineoplásicos/química , Astrócitos/efeitos dos fármacos , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/terapia , Linhagem Celular Tumoral , Células Cultivadas , Dendrímeros/química , Portadores de Fármacos/química , Feminino , Glioblastoma/metabolismo , Glioblastoma/terapia , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Nanopartículas/química , Polilisina/química , RNA Interferente Pequeno/administração & dosagem , Ratos , Espécies Reativas de Oxigênio/metabolismo
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