The 6-week Effects of HIIT on Biomarkers of Tissue and Oxidative Damage in Wistar Rats Previously Supplemented with Pyridoxine.

We aimed to analyze the effects of long high-intensity interval training (HIIT) associated with pyridoxin supplementation on tissue and oxidative injury markers in animals. Male Wistar rats were divided into three groups (n = 8): sedentary (GS), HIIT (GH), and HIIT + pyridoxine (GHP). The HIIT comprised 18 sessions of 7 repetitions of 2min × 2min rest, 3 times per week. Pyridoxine was administered to the GHP group 1h before the exercise. The Thiobarbituric acid reactive substances (TBARS) and sulfhydryl group (SH) were analyzed as markers of oxidative stress and CK, LDH, ALT and AST as tissue lesions. There was an increase in the correlation between CK and LDH of 172.86% and 268.83% in the GH group compared with the GS group, respectively. There was a reduction in CK (34.37%) and LDH (34.74%) compared with the GH group, which had an increase of 229.03% in ALT. Pyridoxine supplementation reduced ALT by 80.62% in the GHP group compared with no-supplementation GH group. In addition, there was a reduction in plasma MDA (52.92%), liver (20.30%) and cardiac (22.06%) tissues in GHP compared to GH. It was possible to conclude that administration of pyridoxine attenuated oxidative stress, and tissue injuries induced by HIIT.

The 6-week Effects of HIIT on Biomarkers of Tissue and Oxidative Damage in Wistar Rats Previously Supplemented with Pyridoxine.

We aimed to analyze the effects of long high-intensity interval training (HIIT) associated with pyridoxin supplementation on tissue and oxidative injury markers in animals. Male Wistar rats were divided into three groups (n = 8): sedentary (GS), HIIT (GH), and HIIT + pyridoxine (GHP). The HIIT comprised 18 sessions of 7 repetitions of 2min × 2min rest, 3 times per week. Pyridoxine was administered to the GHP group 1h before the exercise. The Thiobarbituric acid reactive substances (TBARS) and sulfhydryl group (SH) were analyzed as markers of oxidative stress and CK, LDH, ALT and AST as tissue lesions. There was an increase in the correlation between CK and LDH of 172.86% and 268.83% in the GH group compared with the GS group, respectively. There was a reduction in CK (34.37%) and LDH (34.74%) compared with the GH group, which had an increase of 229.03% in ALT. Pyridoxine supplementation reduced ALT by 80.62% in the GHP group compared with no-supplementation GH group. In addition, there was a reduction in plasma MDA (52.92%), liver (20.30%) and cardiac (22.06%) tissues in GHP compared to GH. It was possible to conclude that administration of pyridoxine attenuated oxidative stress, and tissue injuries induced by HIIT.

Effects of resistance training and turmeric supplementation on reactive species marker stress in diabetic rats.

BACKGROUND: Type 1 diabetes mellitus (T1DM) is a metabolic disease characterized by hyperglycemia and excessive generation of reactive oxygen species caused by autoimmune destruction of beta-cells in the pancreas. Among the antioxidant compounds, Curcuma longa (CL) has potential antioxidant effects and may improve hyperglycemia in uncontrolled T1DM/TD1, as well as prevent its complications (higher costs for the maintenance of health per patient, functional disability, cardiovascular disease, and metabolic damage). In addition to the use of compounds to attenuate the effects triggered by diabetes, physical exercise is also essential for glycemic control and the maintenance of skeletal muscles. Our objective is to evaluate the effects of CL supplementation associated with moderate- to high-intensity resistance training on the parameters of body weight recovery, glycemic control, reactive species markers, and tissue damage in rats with T1DM/TD1. METHODS: Forty male 3-month-old Wistar rats (200-250 g) with alloxan-induced T1DM were divided into 4 groups (n = 7-10): sedentary diabetics (DC); diabetic rats that underwent a 4-week resistance training protocol (TD); CL-supplemented diabetic rats (200 mg/kg body weight, 3x a week) (SD); and supplemented diabetic rats under the same conditions as above and submitted to training (TSD). Body weight, blood glucose, and the following biochemical markers were analyzed: lipid profile, aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid, creatine kinase (CK), lactate dehydrogenase (LDH), and thiobarbituric acid reactive substances (TBARS). RESULTS: Compared to the DC group, the TD group showed body weight gain (↑7.99%, p = 0.0153) and attenuated glycemia (↓23.14%, p = 0.0008) and total cholesterol (↓31.72%, p ≤ 0.0041) associated with diminished reactive species markers in pancreatic (↓45.53%, p < 0.0001) and cardiac tissues (↓51.85%, p < 0.0001). In addition, compared to DC, TSD promoted body weight recovery (↑15.44%, p ≤ 0.0001); attenuated glycemia (↓42.40%, p ≤ 0.0001), triglycerides (↓39.96%, p ≤ 0.001), and total cholesterol (↓28.61%, p ≤ 0.05); and attenuated the reactive species markers in the serum (↓26.92%, p ≤ 0.01), pancreas (↓46.22%, p ≤ 0.0001), cardiac (↓55.33%, p ≤ 0.001), and skeletal muscle (↓42.27%, p ≤ 0.001) tissues caused by T1DM. CONCLUSION: Resistance training associated (and/or not) with the use of Curcuma longa attenuated weight loss, the hypoglycemic and hypolipidemic effects, reactive species markers, and T1DM-induced tissue injury.